Vol 51, No 3 (2013)
Original paper
Submitted: 2012-11-30
Accepted: 2013-08-21
Published online: 2013-11-07
Get Citation

Immunomagnetic detection of cancer cells in pleural effusion of generalized cancer

Grzegorz Kacprzak, Katarina Kolostova, Jerzy Kolodziej, Ireneusz Pawlak, Adam Rzechonek, Vladimir Bobek
DOI: 10.5603/FHC.2013.0028
·
Folia Histochem Cytobiol 2013;51(3):201-205.
Vol 51, No 3 (2013)
ORIGINAL PAPERS
Submitted: 2012-11-30
Accepted: 2013-08-21
Published online: 2013-11-07

Abstract

Malignant pleural effusions (MPE) are a common clinical problem in patients with neoplastic disease. Pleural fluid cytology is the simplest definitive method for obtaining a diagnosis of MPE. We describe a method that may increase the cancer cell detection rate using immunomagnetic separation in MPE. In comparison to standard MPE cytodiagnostic methods, we report a more streamlined method of isolation living cells that are able to proliferate. These captured cells can then be used for additional downstream analysis e.g. chemosensitivity testing. Several case studies of MPE diagnostics using immunomagnetic separation are presented in the following report. The immunomagnetic separation of cancer cells from MPE could be used for more accurate staging of patients with routine effusions.

Abstract

Malignant pleural effusions (MPE) are a common clinical problem in patients with neoplastic disease. Pleural fluid cytology is the simplest definitive method for obtaining a diagnosis of MPE. We describe a method that may increase the cancer cell detection rate using immunomagnetic separation in MPE. In comparison to standard MPE cytodiagnostic methods, we report a more streamlined method of isolation living cells that are able to proliferate. These captured cells can then be used for additional downstream analysis e.g. chemosensitivity testing. Several case studies of MPE diagnostics using immunomagnetic separation are presented in the following report. The immunomagnetic separation of cancer cells from MPE could be used for more accurate staging of patients with routine effusions.

Get Citation

Keywords

pleural effusion; cancer cell isolation; immunomagnetic beads; cell culture

About this article
Title

Immunomagnetic detection of cancer cells in pleural effusion of generalized cancer

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 51, No 3 (2013)

Article type

Original paper

Pages

201-205

Published online

2013-11-07

DOI

10.5603/FHC.2013.0028

Bibliographic record

Folia Histochem Cytobiol 2013;51(3):201-205.

Keywords

pleural effusion
cancer cell isolation
immunomagnetic beads
cell culture

Authors

Grzegorz Kacprzak
Katarina Kolostova
Jerzy Kolodziej
Ireneusz Pawlak
Adam Rzechonek
Vladimir Bobek

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