open access

Vol 50, No 3 (2012)
Original paper
Submitted: 2012-10-08
Accepted: 2012-10-08
Published online: 2012-10-08
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The effect of chronic alcohol intoxication and smoking on the activity of oral peroxidase

Napoleon Waszkiewicz, Anna Zalewska, Sławomir Dariusz Szajda, Agata Szulc, Alina Kępka, Alina Minarowska, Marzena Wojewódzka-Żelezniakowicz, Beata Konarzewska, Sylwia Chojnowska, Zbigniew Bronisław Supronowicz, Jerzy Robert Ładny, Krzysztof Zwierz
DOI: 10.5603/FHC.2012.0062
·
Folia Histochem Cytobiol 2012;50(3):450-455.

open access

Vol 50, No 3 (2012)
ORIGINAL PAPERS
Submitted: 2012-10-08
Accepted: 2012-10-08
Published online: 2012-10-08

Abstract

Peroxidase is the most important antioxidant enzyme in saliva. Through peroxidation of thiocyanate in
the presence of H2O2, peroxidase catalyses the formation of bacteriocidic compounds such as hypothiocyanate.
The purpose of this study was to evaluate the effect of chronic alcohol intoxication and smoking on the activity
of oral peroxidase (OPO). A total of 37 volunteers participated in the study. This cohort consisted of 17 male
alcohol-dependent smoking patients after chronic alcohol intoxication (AS group, alcohol + smoking) (mean
age: 42 years; range: 26–55) (100–700 g/day of alcohol; 10–20 cigarettes/day) and 20 control male social drinkers
(CNS group, control non-smokers) with no history of alcohol abuse or smoking (mean age: 42 years; range:
30–53). Salivary peroxidase activity was measured by the colorimetric method. The differences between groups
were evaluated using the Mann–Whitney U test. There was significantly higher activity of OPO (p = 0.00001)
and significantly lower salivary flow (SF) (p = 0.007) in alcohol-dependent smokers after chronic alcohol intoxication
compared to the control group. OPO activity significantly correlated with the number of days of alcohol
intoxication, but not with smoking. Gingival index (GI) was significantly higher in smoking alcohol-dependent
persons than in the control group, and correlated with OPO activity. The sensitivity of the OPO test was 70% in
smoking alcoholics, while specificity was 95%. The increased activity of OPO suggests chronic oxidative stress is
more likely due to ethanol action than to smoking. Smoking alcohol-dependent persons have a worse periodontal
status than controls. OPO activity as a marker of chronic alcohol abuse may help in the diagnosis of alcoholism.

Abstract

Peroxidase is the most important antioxidant enzyme in saliva. Through peroxidation of thiocyanate in
the presence of H2O2, peroxidase catalyses the formation of bacteriocidic compounds such as hypothiocyanate.
The purpose of this study was to evaluate the effect of chronic alcohol intoxication and smoking on the activity
of oral peroxidase (OPO). A total of 37 volunteers participated in the study. This cohort consisted of 17 male
alcohol-dependent smoking patients after chronic alcohol intoxication (AS group, alcohol + smoking) (mean
age: 42 years; range: 26–55) (100–700 g/day of alcohol; 10–20 cigarettes/day) and 20 control male social drinkers
(CNS group, control non-smokers) with no history of alcohol abuse or smoking (mean age: 42 years; range:
30–53). Salivary peroxidase activity was measured by the colorimetric method. The differences between groups
were evaluated using the Mann–Whitney U test. There was significantly higher activity of OPO (p = 0.00001)
and significantly lower salivary flow (SF) (p = 0.007) in alcohol-dependent smokers after chronic alcohol intoxication
compared to the control group. OPO activity significantly correlated with the number of days of alcohol
intoxication, but not with smoking. Gingival index (GI) was significantly higher in smoking alcohol-dependent
persons than in the control group, and correlated with OPO activity. The sensitivity of the OPO test was 70% in
smoking alcoholics, while specificity was 95%. The increased activity of OPO suggests chronic oxidative stress is
more likely due to ethanol action than to smoking. Smoking alcohol-dependent persons have a worse periodontal
status than controls. OPO activity as a marker of chronic alcohol abuse may help in the diagnosis of alcoholism.
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About this article
Title

The effect of chronic alcohol intoxication and smoking on the activity of oral peroxidase

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 50, No 3 (2012)

Article type

Original paper

Pages

450-455

Published online

2012-10-08

DOI

10.5603/FHC.2012.0062

Bibliographic record

Folia Histochem Cytobiol 2012;50(3):450-455.

Authors

Napoleon Waszkiewicz
Anna Zalewska
Sławomir Dariusz Szajda
Agata Szulc
Alina Kępka
Alina Minarowska
Marzena Wojewódzka-Żelezniakowicz
Beata Konarzewska
Sylwia Chojnowska
Zbigniew Bronisław Supronowicz
Jerzy Robert Ładny
Krzysztof Zwierz

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