Introduction. Left ventricular diastolic dysfunction (LVDD) is described as impaired left ventricular (LV) relaxation and reduced chamber compliance. Misleading data on the prevalence of LVDD are available in the literature due to various definitions. This study aimed to assess the frequency of LVDD in a population without severe cardiovascular disease (CVD), as well as to identify factors associated with it. Material and methods. Overall, 648 individuals without severe CVD were included. LVDD was assessed using the last 2016 guidelines (LVDD2016) together with the previous recommendations from 1998 (LVDD1998). Results. In total, 35 participants (5.4%) met the LVDD2016 criteria, and 29 people (4.5%) fulfilled only the LVDD1998 criteria. The strongest factors independently associated with LVDD2016 were body mass index (BMI), high-sensitivity C-reactive protein, high-sensitivity troponin T, ejection fraction and circumference of neck and waist. LVDD2016 presents a significant association with the anthropometric measures (BMI, neck and waist circumference), LV function and overload as well as the inflammatory parameter. Conclusions. In the population without overt CVD the frequency of LVDD as defined by the latest 2016 guidelines is 5.4%. It was associated with inflammatory, cardiac damage and anthropometric parameters.