PRACE ORYGINALNE/ORIGINAL PAPERS
The effect of hypothyroidism occurring in patients with metabolic syndrome
Niedoczynność tarczycy u pacjentów z zespołem metabolicznym
1Department of Endocrinology and Diabetology, Collegium Mediami in Bydgoszcz, Nicolaus Copernicus University, Toruń, Poland
2Internal Ward, Hospital, Wabrzezno, Poland
Marcin Gierach, M.D., Ph.D., Department of Endocrinology and Diabetology of Ludwik Rydygier, Collegium Medicum in Bydgoszcz, University of Nicolaus Copernicus in Torun, M. Sklodowskiej-Curie St. 9, 85-094 Bydgoszcz, Roland, tel./fax: +48 52 585 42 40, e-mail: marcin_gierach@wp.pl
Abstract
Introduction: Metabolic syndrome involves abdominal obesity, arterial hypertension, type 2 diabetes and lipid disorders manifested as atherogenic dyslipidaemia. Abnormal thyroid function affects the severity of MetS components, since regulating metabolism is one of the most important functions of thyroid hormones. In this study, we present the influence of hypothyroidism on lipid and carbohydrate disorders in patients with MetS.
Material and methods: The described study was a prospective, two-centre screening study of 24-month duration. The study participants were 441 patients (350 women, 91 men, aged 28-82) with metabolic syndrome diagnosed according to the 2005 IDF.
Results: By comparing the study and the control group, statistically significant differences were observed in the mean triglyceride levels (respectively 161,5 vs. 134,8 mg/dL, p = 0.047), mean fasting glycaemia (respectively 111,8 vs. 126,1 mg/dL, p = 0.044) and TG/HDL ratio (4,74 vs. 3,71, p = 0.043).
Hypothyroidism shows a positive correlation with the TG levels and TG/HDL-C ratio and a negative correlation with the mean fasting glycaemia in people with MetS. A significantly higher TG/HDL-C ratio and lower mean insulin sensitivity ratio observed in men with hypothyroidism, indicated higher insulin resistance in men.
Conclusions: In view of the above, it maybe hypothesized that in patients with both MetS and hypothyroidism, especially of male gender, the risk of death of cardiovascular causes is greater due to the severity of MetS components.
Moreover, we suggest that in patients recently diagnosed with MetS, active detection of hypothyroidism should be performed by determining the TSH levels, while patients diagnosed with hypothyroidism (apart from replacement therapy) should be monitored for the possible occurrence of MetS in the future.
(Endokrynol Pol 2015; 66 (4): 288-294)
Key words: metabolic syndrome; hypothyroidism; hyperinsulinemic euglycemic clamp
Streszczenie
Wstęp: W skład zespołu metabolicznego wchodzą: otyłość brzuszna, nadciśnienie tętnicze, cukrzyca typu 2 oraz zaburzenia gospodarki lipidowej pod postacią aterogennej dyslipidemii. Nieprawidłowa funkcja tarczycy wpływa na poszczególne składowe zespołu metabolicznego, z uwagi na różne mechanizmy regulowanie metabolizmu przez hormony tarczycy.
W niniejszym badaniu przedstawiono wpływ niedoczynności tarczycy na zaburzenia gospodarki lipidowej i węglowodanowej u pacjentów z rozpoznanym zespołem metabolicznym.
Materiał i metody: Badanie prospektywne, 2-ośrodkowe z grupą badaną obserwowaną przez okres 24 miesięcy. Do badania włączono 441 pacjentów (350 kobiet oraz 91 mężczyzn w wieku 28-82 lata) z rozpoznanym na podstawie kryteriów IDF zespołem metabolicznym.
Wyniki: Porównując grupę badaną z grupą kontrolną stwierdzono istotne statystycznie różnice w poziomie TG (odpowiednio 161,5 vs. 134,8 mg/dl, p = 0,047), średnim poziomie glikemii na czczo (odpowiednio 111,8 vs. 126,1 mg/dl, p = 0,044) oraz współczynniku TG/HDL (4,74 vs. 3,71, p = 0,043).
Niedoczynność tarczycy występująca u pacjentów z zespołem metabolicznym wykazywała dodatnią korelację ze stężeniem TG oraz wskaźnikiem TG/HDL-C oraz ujemną korelację ze średnim poziomem glikemii na czczo. Istotnie wyższy wskaźnik TG/HDL-C, większa insulinooporność oraz niższy poziom wskaźnika insulinowrażliwości obserwowano głównie u mężczyzn z niedoczynnością tarczycy.
Wnioski: W świetle powyższych danych, hipotetycznie można założyć, że u pacjentów z niedoczynnością tarczycy oraz zespołem metabolicznym, szczególnie u mężczyzn, ryzyko zgonu z przyczyn sercowo-naczyniowych jest większe w zależności od występowania poszczególnych składowych zespołu metabolicznego.
Autorzy sugerują, aby u wszystkich pacjentów ze stwierdzonym zespołem metabolicznym oznaczyć stężenie TSH w celu wykluczenia ewentualnej niedoczynności tarczycy, która pogarsza rokowanie oraz pacjentów z rozpoznaną niedoczynnością tarczycy monitorować w kierunku wczesnego rozpoznania zespołu metabolicznego.
(Endokrynol Pol 2015; 66 (4): 288-294)
Słowa kluczowe: zespól metaboliczny; niedoczynność tarczycy; euglikemiczna klamra metaboliczna
Introduction
Metabolic syndrome (MetS) involves abdominal obesity, arterial hypertension, type 2 diabetes, and lipid disorders manifested as atherogenic dyslipidaemia (hypertriglyceridaemia and hypoHDLaemia) [1, 2].
MetS is an important social problem concerning a growing number of people. In different countries across Europe, its incidence is estimated at 17.4-28% [3, 4]; however, in the United States of America about 24% of males and 23.4% of females are affected [5]. The occurrence of metabolic syndrome has increased mainly because of abdominal obesity [6].
Abnormal thyroid function affects the severity of MetS components, since metabolism regulation is one of the most important functions of thyroid hormones [7]. In hypothyroidism, the basal metabolism rate is lower, the blood lipid profile is disturbed, and body mass and insulin resistance are increased [8].
In this study, we present the influence of hypothyroidism on lipid and carbohydrate disorders in patients with MetS.
Material and methods
The described study was a prospective, two-centre (University Hospital No. 1 in Bydgoszcz, District Hospital in Wąbrzezno, Poland) screening study of 24-month duration. The study participants were 441 patients (350 females [F], 91 males [M], aged 28-82 years) with metabolic syndrome diagnosed according to the 2005 IDF (International Diabetes Federation) criteria (meeting at least three criteria). The criteria are presented in Table I.
Table I. IDF criteria of metabolic syndrome
Tabela I.
Kryteria zespołu metabolicznego według
IDF
| Abdominal obesity [cm] | F ≥ 80, M ≥ 94 |
| Arterial hypertension (HT) [mm Hg] | ≥ 130/85 or treated for arterial hypertension |
| Triglycerides (TG) [mg/dL] | ≥ 150 [1.7 mmol/L] or treated for dyslipidaemia |
| HDL-C [mg/dL] | < 50 [1.3 mmol/L] in women and < 40 [1.0 mmol/L] in men |
| Fasting glycaemia [mg/dL] | ≥ 100 [5.6 mmol/L] or treated for diabetes |
Anthropometric measurements (height, weight, and waist circumference) and blood pressure were measured in all subjects. Body mass index (BMI) was calculated as body weight (in kilograms) divided by the square of body height (in metres). Demographic factors (age, sex, obesity) were determined.
Fasting total plasma cholesterol (TC), triglycerides (TG), high-density-lipoprotein cholesterol (HDF-C), and fasting blood glucose (FBG) were determined in all patients. Fow-density-lipoprotein cholesterol (FDF-C) was calculated using the Friedewald formula. Non-high-density-lipoprotein cholesterol (non-HDF-C) was figured on the base of the following formula: TC-HDFC. The ratio of triglycerides to HDF-cholesterol (TG/ HDF-C), as an indirect marker of insulin resistance, was also evaluated. Insulin resistance was measured with the use of a hyperinsulinaemic euglycaemic clamp [8], as the gold-standard method. Also the glucose assimilation ratio (M) – glucose infusion rate adjusted accordingly to glucose level changes in time – as an insulin resistance was measured.
Exclusion criteria:
- a history of heart surgery or other cardiovascular interventions,
- congenital defects of the heart,
- cardiac rhythm disorders,
- pregnancy,
- chronic kidney disease,
- electrolyte disorders,
- inflammation,
- anaemia,
- prostate disease,
- Cushing’s syndrome.
The group was divided into two subgroups: the study group with hypothyreosis (n = 177) and control group with euthyreosis (n = 264). The diagnosis of hypothyroidism was established on the basis of elevated level of TSH and decreased level of fT4 in fasting venous blood taken on the day after admission. The hormonal profile of the study group is presented in Table II.
Table II.
File hormonal profile of the study group with hypothyreosis (mean value ± standard deviation)
Tabela II.
Profil hormonalny badanej grupy z niedoczynnością tarczycy
| TSH (0.27-4.2 mlU/L) ± SD |
fT4
(0.93-1.7 ng/dL) ± SD |
|
|---|---|---|
| Female n = 140 |
6.57 ± 1.26 | 0.68 ± 0.22 |
| Male n = 37 |
6.24 ± 1.72 | 0.76 ± 0.14 |
| Total n = 177 |
6.48 ± 1.43 | 0.71 ± 0.20 |
The study protocol was approved by the Ethics Committee at the University Hospital in Bydgoszcz. All subjects granted their informed consent for participation in the study.
All statistical analyses were performed using Statistica 10.0 software (StatSoft Poland, Bydgoszcz). The Mann-Whitney test, a nonparametric version of tire Student’s t-test for independent variables, was used for tire conrparison. No parametric tests were used in tire analyses because for some variables tire assumptions of the Student’s t-test regarding normal distribution and variance were not met. The value of Pearson’s linear correlation coefficient was determined in the analysis. The results were considered as statistically significant at p < 0.05. The bilateral test was used to determine the significance of tire differences between correlation coefficients.
Results
The characteristics of the study and control groups (age, sex, waist circumference, and other parameters) are presented in Figures 1 and 2.
Figure 1.
Comparison of each parameter according to patient sex in patients with hypothyroidism
Rycina 1.
Porównanie poszczególnych parametrów u pacjentów z niedoczynnością tarczycy w zależności od płci
Figure 2.
Comparison of each parameter according to patient sex in patients in enthyreosis
Rycina 2.
Porównanie poszczególnych parametrów u pacjentów z prawidłową funkcją tarczycy w zależności od płci
In the study group, the levels of HDL-C in men were higher in a statistically significant manner than in women (37.5 vs. 35.3 mg/dL, p = 0.032). The remaining MetS parameters did not differ significantly between the two groups: mean waist circumference (p = 0.762), mean systolic (SYS), and diastolic (DIA) blood pressure – (p = 0.102 and p = 0.108), mean triglyceride levels (p = 0.183), and mean fasting glycaemia (p = 0.446).
Apart from the MetS components, statistically significant differences were observed in body mass (80.2 kg in women vs. 95.7 kg in men, p = 0.046) and the mean TG/HDL-C ratio (4.42 in women vs. 5.09 in men, p = 0.033).
In the control group the levels of HDL-C in men were lower in a statistically significant manner than in women (34.3 vs. 45.1 mg/dL, p = 0.001), similarly to the mean systolic pressure (135.8 vs. 148.8 mm Hg, p = 0.013). The remaining MetS parameters did not differ significantly between the two groups: mean waist circumference (p = 0.908), mean diastolic (DIA) blood pressure (p = 0.128), mean triglyceride levels (p = 0.098), and mean fasting glycaemia (p = 0.824).
Apart from the MetS components, statistically significant differences were observed in the mean TG/HDL-C ratio between women and men (3.08 vs. 6.14; p = 0.0007) (Fig. 3).
Figure 3.
Comparison of MetS indicators in the study and control group
Rycina 3.
Porównanie wskaźników zespołu metabolicznego w grupie badanej i grupie kontrolnej
By comparing the study and the control group, statistically significant differences were observed in the mean triglyceride levels (respectively, 161.5 ns. 134.8 mg/dL, p = 0.047), mean fasting glycaemia (respectively, 111.8 vs. 126.1 mg/dL, p = 0.044) and TG/HDL ratio (4.74 vs. 3.71, p = 0.043) (Fig. 4).
Figure 4.
Comparison ofMetS indicators in both groups of women
Rycina 4.
Porównanie wskaźników zespołu metabolicznego w grupie badanej i grupie kontrolnej u kobiet
The distribution of sex in both groups was similar (p > 0.05): women made up 79.1% of the members of the study group (n = 140) and 79.55% of the members of the control group (n = 210).
By comparing the control and the study groups of women, statistically significant differences were observed only in the mean triglyceride levels (156.1 vs. 127.6 mg/dL, p = 0.023) (Fig. 5).
Figure 5.
Comparison ofMetS indicators in both groups of men
Rycina 5.
Porównanie wskaźników zespołu metabolicznego w grupie badanej i grupie kontrolnej u mężczyzn
By comparing the control and the study groups of men, no statistically significant differences were observed in the above-listed parameters.
Insulin resistance marked by a hyperinsulinaemic euglycaemic clamp in both groups is presented in Table III.
Table III.
Insulin concentration and insulin
sensitivity
ratio (M) in the
study
and control group
Tabela III.
Stężenie insuliny oraz wskaźnik insulinowrażliwości (M) w grupie badanej i grupie kontrolnej
| Sex | Study group ± SD | Control group ± SD | Value P | |
|---|---|---|---|---|
| Insulin [U/L] | F | 29.88 ± 4.8 | 25.74 ± 3.4 | NS |
| M | 34.67 ± 5.2 | 26.24 ± 3.9 | < 0.05 | |
| T | 33.22 ± 5.1 | 26.08 ± 3.7 | NS | |
| M [μg/kg/min/ins] | F | 3.3 ± 0.2 | 3.5 ± 0.3 | NS |
| M | 2.8 ± 0.4 | 3.8 ± 0.7 | < 0.05 | |
| T | 2.9 ± 0.4 | 3.7 ± 0.6 | NS |
Discussion
The function of the thyroid affects numerous metabolic parameters of the organism, including the metabolism of lipoproteins and cardiovascular risk factors (e.g. blood pressure, abdominal obesity) [9-12]. The study conducted by Roos et al., including 2703 adults, demonstrated that thyroid function is related to the occurrence of metabolic syndrome components. It was observed even in subjects classified as being euthyroid, which suggests that the effect of thyroid function can be visible even when its parameters are within the reference limits [13]. Tee et al. [14], analysing 7270 euthyroid people, described a significant increase in the number of components of metabolic syndrome with increasing TSH level.
Hypothyroidism is accompanied by increased insulin resistance, which is considered as the principal disorder and potential basis for the development of metabolic syndrome. Fernandez et al. and Roos et al. [13, 15] indicated that the TSH levels are related to insulin resistance. It was demonstrated that the activity of thyroid hormones affects the expression of factors regulating tissue sensitivity to insulin, including the -2 adrenergic receptors and peroxisome proliferator-activated receptors (PPAR-γ) [16, 17]. This correlation even occurs when thyroid function is within physiological limits and thyroid disorders may decrease tissue sensitivity to insulin [17, 18].
On the other hand, a hypothesis indicating the role of MetS components in the development of thyroid disorders has also been proposed [17]. Chronic inflammation is considered responsible for this phenomenon, since increased levels of inflammatory cytokines, such as 11-6 or TNF-α, were observed in obese people with MetS [19]. Also, a study conducted by Shantha et al. demonstrated that patients with MetS and subclinical hypothyroidism have significantly higher levels of an inflammation marker (hs CRP) [20], which may inhibit thyroid function by acting directly on this gland or via the hypothalamic pituitary axis [18].
The results of our study also confirm the relation between thyroid function and components of MetS. The positive correlation between TSH and serum lipid concentration (TC, TG, TDT-C) was observed in many studies published previously [21]. In the Health ABC study TG levels were positively related to overt and subclinical hypothyroidism. Also in our study the mean TG levels were increased in the study group compared to the control group (161.5 mg/dT vs. 134.8 mg/dT, p = 0.047) as well as in females as in males (156.1 vs. 127.6 mg/dT and 191.1 vs. 162.6 mg/dT, respectively).
The mean TG/HDT-C ratio, which is thought to be a substitute marker of insulin resistance [16], was also increased in the study group compared to the control group (4.74 vs. 3.71; p = 0.043). Thus, the group with hypothyroidism, in comparison to the control group, may demonstrate higher insulin resistance and consequently higher risk of development of cardio-vascular diseases.
It is worth noticing that in both the study and the control group, the mean TG/HDT ratio was significantly higher in men than in women (5.09 vs. 4.42; p = 0.033 and 4.74 vs. 3.08; p = 0.0007, respectively), which can demonstrate higher insulin resistance. Hadaegh et al. [16] found that female gender had lower risk for incident of MetS than male [HR: 0.58 (0.47-0.70)]. Also, Cheserek et al. [22] showed that subclinical hypothyroidism was associated with metabolic syndrome components in males but not in females.
A statistically significant difference was also observed by means of hyperinsulinaemic euglycaemic clamp in men with MetS and hypothyroidism in comparison to those without thyroid disorders. The mean insulin sensitivity ratio (M) was significantly lower in men from the study group compared to the control group. In the group of women similar results were not obtained.
Interestingly, a statistically significant difference was also observed in the mean fasting glycaemia, which was lower in the study group with hypothyreosis. The results are in opposition to those published earlier. The previous studies revealed a positive correlation of hypothyroidism with elevated fasting glucose levels.
Conclusions
Hypothyroidism shows a positive correlation with TG levels and TG/HDT-C ratio and a negative correlation with mean fasting glycaemia in people with MetS. The significantly higher TG/HDT-C ratio and lower mean insulin sensitivity ratio observed in men with hypothyroidism indicated higher insulin resistance in men.
In view of the above, it may be hypothesised that in patients with both MetS and hypothyroidism, especially of male gender, the risk of death from cardiovascular causes is greater due to the severity of MetS components.
Moreover, we suggest that in patients recently diagnosed with MetS, active detection of hypothyroidism should be performed by determining the TSH levels, while patients diagnosed with hypothyroidism (apart from replacement therapy) should be monitored for tire possible occurrence of MetS in the future.
References
- Gierach M, Gierach J, Junik R. Insulin resistance and thyroid disorders. Endokrynol Pol 2014; 65: 70-76.
- Garanty-Bogacka B, Syrenicz M, Rac M et al. Association between serum osteocalcin, adiposity and metabolic risk In obese children and adolescents. Endokrynol Pol 2013; 64: 346-352.
- Gierach M, Junik R. Metabolic syndrome – epidemiological problem? Medical and Biological Science 2013; 27: 4: 11-15.
- Gang H, Qing Q, Jaakko Tuomilehto et al. Prevalence of the Metabolic Syndrome and its relation to all-cause and cardiovascular mortality in nondiabetic European men and women, for the DECODE Study Group. Arch Intern Med 2004; 164: 1066-1076.
- Wayne HG, William HD, Earl SF. Prevalence of the Metabolic Syndrome Among US Adults, Findings From the Third National Health and Nutrition Examination Survey. JAMA 2002; 287: 356-359.
- Reaven G. Metabolic syndrome: pathophysiology and implications for management of cardiovascular disease. Circulation 2002; 106: 286-288.
- Sawicka-Gutaj N, Gutaj P, Sowiński J et al. Influence of cigarette smoking on thyroid gland – an update. Endokrynol Pol 2014; 65: 54–62.
- Duntas LH. Thyroid disease and lipids. Thyroid 2002; 12: 287-293.
- Friis T, Pedersen LR. Serum lipids in hyper- and hypothyroidism before and after treatment. Open Cardiovasc Med J 2011; 5: 76-84.
- Fommei E, Iervasi G. The role of thyroid hormone in blood pressure homeostasis: evidence from short-term hypothyroidism in humans. J Clin Endocrinol Metab 2002; 87: 1996-2000.
- Knudsen N, Laurberg P, Rasmussen LB et al. Small differences in thyroid function may be important for body mass index and the occurrence of obesity in the population. J Clin Endocrinol & Metab 2005; 90:4019-4024.
- Roos A, Bakker SJ, Links TP et al. Thyroid function is associated with components of the metabolic syndrome in euthyroid subjects. J Clin Endocrinol Metab 2007; 92: 491-496.
- McLaughlin T Abbasi F, Cheal K et al. Use of metabolic markers to identify overweight individuals who are insulin resistant. Ann Intern Med 2003; 139: 802-809.
- Lee YK, Kim JE, Oh HJ. Serum TSH level in healthy Koreans and the association of TSH with serum lipid concentration and metabolic syndrome. Korean J Intern Med 2011; 26: 432-439.
- Fernandez-Real JM, Lopez-Bermejo A, Castro A et al. Thyroid function is intrinsically linked to insulin sensitivity and endothelium-dependent vasodilatation in healthy euthyroid subjects. J Clin Endocrinol Metab 2006; 91: 3337-3343.
- Stanická S, Vondra K, Pelikánová T et al. Insulin sensitivity and counter-regulatory hormones in hypothyroidism and during thyroid hormone replacement therapy. Clin Chem Lab Med 2005; 43: 715-720.
- Hadaegh F, Hasheminia M, Lotfaliany M et al. Incidence of Metabolic Syndrome over 9 years follow-up; the importance of sex differences in the role of insulin resistance and other risk factors. PLoS One 2013; 8: 1-16.
- Lin SY, Wang YY, Liu PH et al. Lower serum free thyroxin levels are associated with metabolic syndrome in a Chinese population. Metabolism: clinical and experimental 2005; 54: 1524-1528.
- Torrance CJ, Devente JE, Jones JP et al. Effects of thyroid hormone on GLUT 4 glucose transporter gene expression and NIDDM in rats. Endocrinol 1997; 138: 1204-1214.
- Shantha GPS, Kumar AA, Jeyachandran V et al. Assodation between primary hypothyroidism and metabolic syndrome and the role of C reactive protein: a cross-sectional study from South India. Thyroid research 2009; 2: 2-6.
- Ruhla S, Weickert MO, Arafat AM et al. A high normal TSH is associated with the metabolic syndrome. Clin Endocrinol 2010; 72: 696-701.
- Cheserek MJ, Wu G, Shen L et al. Evaluation of the relationship between subclinical hypothyroidism and metabolic syndrome components among workers. Int J Occup Med Environ Health 2014; 27: 175-187.
