open access

Vol 73, No 2 (2022)
Original paper
Submitted: 2021-12-15
Accepted: 2022-01-09
Published online: 2022-04-13
Get Citation

Astragaloside IV drug-loaded exosomes (AS-IV EXOs) derived from endothelial progenitor cells improve the viability and tube formation in high-glucose impaired human endothelial cells by promoting miR-214 expression

Xiaoling Zou1, Hui Xiao1, Xue Bai1, Yixian Zou1, Wenxiao Hu1, Xiangdong Lin1, Chenhong Zhu2, Yao Liang2, Wu Xiong3
·
Pubmed: 35593682
·
Endokrynol Pol 2022;73(2):336-345.
Affiliations
  1. Department of Endocrinology, the First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan, China
  2. College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, Hunan, China
  3. Department of Burns and Plastic Surgery, the First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan, China

open access

Vol 73, No 2 (2022)
Original Paper
Submitted: 2021-12-15
Accepted: 2022-01-09
Published online: 2022-04-13

Abstract

Introduction: The high glucose changes caused by diabetes mellitus (DM) can damage the vascular system. Astragaloside IV (AS-IV) can improve diabetes and promote angiogenesis. Exosomes (EXOs) help to carry specific drugs into cells efficiently. However, whether AS-IV loaded EXOs (AS-IV EXOs) can improve damaged endothelial cells through miR-214 remains to be determined.

Material and methods: We prepared and identified AS-IV EXOs derived from endothelial progenitor cells (EPCs) and high glucose stimulated endothelial cell models to investigate whether AS-IV EXOs can improve damaged endothelial cells through miR-214. We used a transmission electron microscope (TEM) and DAPI staining to identify the morphology and characteristic expression of EPCs and EXOs, and then prepared AS-IV EXOs. Cell function tests were performed to detect the cloning, proliferation, and migration capabilities of cells. Western blot (WB) and real-time quantitative polymerase chain reaction (qRT-PCR) were used to assess the expression level of Tie-2, Ang-1, and PI3K/Akt-related protein.

Results: The DAPI staining results showed that inducing human umbilical vein endothelial cells (HUVECs) could effectively absorb AS-IV EXOs. The results of plate clone formation assay, CCK-8, cell adhesion, and transwell assay of HUVECs stimulated by high glucose showed that AS-IV EXOs had a damage relief effect. By the detection of WB and qRT-PCR, it was found that AS-IV EXOs promoted the expression of miR-214 and proteins related to blood vessel growth. After transfection of miR-214 to pre-treat HUVECs under high glucose stimulation, AS-IV EXOs promoted the tube formation of HUVECs by regulating the level of miR-214.

Conclusions: By promoting the expression of miR-214, AS-IV EXOs significantly improved the activity and tubularization of HUVECs under high glucose stimulation.

Abstract

Introduction: The high glucose changes caused by diabetes mellitus (DM) can damage the vascular system. Astragaloside IV (AS-IV) can improve diabetes and promote angiogenesis. Exosomes (EXOs) help to carry specific drugs into cells efficiently. However, whether AS-IV loaded EXOs (AS-IV EXOs) can improve damaged endothelial cells through miR-214 remains to be determined.

Material and methods: We prepared and identified AS-IV EXOs derived from endothelial progenitor cells (EPCs) and high glucose stimulated endothelial cell models to investigate whether AS-IV EXOs can improve damaged endothelial cells through miR-214. We used a transmission electron microscope (TEM) and DAPI staining to identify the morphology and characteristic expression of EPCs and EXOs, and then prepared AS-IV EXOs. Cell function tests were performed to detect the cloning, proliferation, and migration capabilities of cells. Western blot (WB) and real-time quantitative polymerase chain reaction (qRT-PCR) were used to assess the expression level of Tie-2, Ang-1, and PI3K/Akt-related protein.

Results: The DAPI staining results showed that inducing human umbilical vein endothelial cells (HUVECs) could effectively absorb AS-IV EXOs. The results of plate clone formation assay, CCK-8, cell adhesion, and transwell assay of HUVECs stimulated by high glucose showed that AS-IV EXOs had a damage relief effect. By the detection of WB and qRT-PCR, it was found that AS-IV EXOs promoted the expression of miR-214 and proteins related to blood vessel growth. After transfection of miR-214 to pre-treat HUVECs under high glucose stimulation, AS-IV EXOs promoted the tube formation of HUVECs by regulating the level of miR-214.

Conclusions: By promoting the expression of miR-214, AS-IV EXOs significantly improved the activity and tubularization of HUVECs under high glucose stimulation.

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Keywords

astragaloside IV; endothelial cells; miR-214; exosomes; tube formation; diabetes mellitus

About this article
Title

Astragaloside IV drug-loaded exosomes (AS-IV EXOs) derived from endothelial progenitor cells improve the viability and tube formation in high-glucose impaired human endothelial cells by promoting miR-214 expression

Journal

Endokrynologia Polska

Issue

Vol 73, No 2 (2022)

Article type

Original paper

Pages

336-345

Published online

2022-04-13

Page views

5654

Article views/downloads

1062

DOI

10.5603/EP.a2022.0011

Pubmed

35593682

Bibliographic record

Endokrynol Pol 2022;73(2):336-345.

Keywords

astragaloside IV
endothelial cells
miR-214
exosomes
tube formation
diabetes mellitus

Authors

Xiaoling Zou
Hui Xiao
Xue Bai
Yixian Zou
Wenxiao Hu
Xiangdong Lin
Chenhong Zhu
Yao Liang
Wu Xiong

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