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Ahead of print
Original paper
Published online: 2021-05-31
Submitted: 2021-02-10
Accepted: 2021-05-06
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Serum zonulin levels in type 2 diabetes patients with diabetic kidney disease

Fevziye Burcu Sirin, Hakan Korkmaz, Ismet Eroglu, Barıs Afsar, Duygu Kumbul Doguc
DOI: 10.5603/EP.a2021.0056
·
Pubmed: 34057189

open access

Ahead of print
Original Paper
Published online: 2021-05-31
Submitted: 2021-02-10
Accepted: 2021-05-06

Abstract

Introduction: Recent data has shown that diabetic kidney disease is associated with abnormal gut microbiota composition. Zonulin is a physiological tight junction modulator and an intestinal permeability marker. In this study we aimed to investigate serum levels of zonulin and interleukin 6 (IL-6) in patients with type 2 diabetes mellitus and different levels of albuminuria. Material and Methods: Ninety patients with T2DM and 30 healthy controls (HC) aged between 18 and 65 years were enrolled in the study. T2DM patients were divided into three groups as patients with normoalbuminuria (n=30), microalbuminuria (n=30), and macroalbuminuria (n=30). Serum zonulin and IL-6 levels were measured by ELISA method. Results: There was no significant difference between groups for age, gender, serum ALT, LDL-C, HDL-C and zonulin levels (p>0.05). Significant differences between groups were present for the duration of diabetes (p<0.001), body mass index (p<0.001), fasting blood glucose (p<0.001), creatinine (p<0.001), uric acid (p=0.037), triglyceride (p=0.003), total cholesterol (p<0.001), glycated hemoglobin (p<0.001) and IL-6 (p<0.001) levels. IL-6 levels were significantly increased in microalbuminuria and macroalbuminuria groups compared to the HC group but no significant difference was determined between HC and normoalbuminuria group. In patients with diabetic kidney disease, a significant positive correlation was found between zonulin with IL-6 and proteinuria (rho= 0.296, p=0.008; rho=0.190, p<0.047 respectively). Serum IL-6 level was positively correlated with microalbuminuria and proteinuria (rho=0.451, p<0.001; rho=0.425, p<0.001 respectively). Conclusions: We suggest that serum zonulin level is not a promising biomarker to assess the severity of DKD in patients with long standing T2DM.

Abstract

Introduction: Recent data has shown that diabetic kidney disease is associated with abnormal gut microbiota composition. Zonulin is a physiological tight junction modulator and an intestinal permeability marker. In this study we aimed to investigate serum levels of zonulin and interleukin 6 (IL-6) in patients with type 2 diabetes mellitus and different levels of albuminuria. Material and Methods: Ninety patients with T2DM and 30 healthy controls (HC) aged between 18 and 65 years were enrolled in the study. T2DM patients were divided into three groups as patients with normoalbuminuria (n=30), microalbuminuria (n=30), and macroalbuminuria (n=30). Serum zonulin and IL-6 levels were measured by ELISA method. Results: There was no significant difference between groups for age, gender, serum ALT, LDL-C, HDL-C and zonulin levels (p>0.05). Significant differences between groups were present for the duration of diabetes (p<0.001), body mass index (p<0.001), fasting blood glucose (p<0.001), creatinine (p<0.001), uric acid (p=0.037), triglyceride (p=0.003), total cholesterol (p<0.001), glycated hemoglobin (p<0.001) and IL-6 (p<0.001) levels. IL-6 levels were significantly increased in microalbuminuria and macroalbuminuria groups compared to the HC group but no significant difference was determined between HC and normoalbuminuria group. In patients with diabetic kidney disease, a significant positive correlation was found between zonulin with IL-6 and proteinuria (rho= 0.296, p=0.008; rho=0.190, p<0.047 respectively). Serum IL-6 level was positively correlated with microalbuminuria and proteinuria (rho=0.451, p<0.001; rho=0.425, p<0.001 respectively). Conclusions: We suggest that serum zonulin level is not a promising biomarker to assess the severity of DKD in patients with long standing T2DM.

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Keywords

type 2 diabetes mellitus; diabetic kidney disease; intestinal permeability; zonulin; inflammation

About this article
Title

Serum zonulin levels in type 2 diabetes patients with diabetic kidney disease

Journal

Endokrynologia Polska

Issue

Ahead of print

Article type

Original paper

Published online

2021-05-31

DOI

10.5603/EP.a2021.0056

Pubmed

34057189

Keywords

type 2 diabetes mellitus
diabetic kidney disease
intestinal permeability
zonulin
inflammation

Authors

Fevziye Burcu Sirin
Hakan Korkmaz
Ismet Eroglu
Barıs Afsar
Duygu Kumbul Doguc

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