open access

Vol 72, No 2 (2021)
Original paper
Published online: 2020-12-08
Submitted: 2020-09-02
Accepted: 2020-10-12
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Chromosomal and oxidative DNA damage in non-functioning pituitary adenomas

Nazmiye Bitgen, Fahri Bayram, Zuhal Hamurcu, Figen Ozturk, Yasin Simsek, Gulden Baskol, Ali Kurtsoy, Hamiyet Donmez-Altuntas
DOI: 10.5603/EP.a2020.0084
·
Pubmed: 33295635
·
Endokrynologia Polska 2021;72(2):97-103.

open access

Vol 72, No 2 (2021)
Original Paper
Published online: 2020-12-08
Submitted: 2020-09-02
Accepted: 2020-10-12

Abstract

Introduction: Clinically non-functioning pituitary adenomas (NFPA) are common tumours of the pituitary gland and are mainly considered as benign. The primary aim of this study was to research the effects of NFPA on genome instability in patients with non-functioning pituitary adenoma by using the cytokinesis-block micronucleus cytome (CBMN-cyt) assay and 8-hydroxy- 2’-deoxyguanosine (8-OHdG) assay. The second objective of this study was to assess whether there is a relationship between age, pituitary adenoma diameters, 8-OHDG levels, CBMN site assay parameters, and tumour aggressiveness.

Material and methods: The study was performed on 30 patients who had been diagnosed with NFPA and were admitted to the Department of Endocrinology and Metabolism, and 20 healthy subjects of similar age and sex.

Results: Micronucleus (MN), nucleoplasmic bridges (NPBs), nuclear bud (NBUD) frequencies, and apoptotic and necrotic cell frequencies in patients with NFPA were found to be significantly higher than in control subjects, and plasma 8-OHdG levels in patients with NFPA were statistically significantly lower than control subjects in this study.

Conclusions: It is believed that this is the first study to evaluate the aggressiveness of tumour with chromosome/oxidative DNA damage in patients with NFPA. However, further studies are needed in order to understand the cause of NFPA aggression and to evaluate these patients in terms of risk of cancer. 

Abstract

Introduction: Clinically non-functioning pituitary adenomas (NFPA) are common tumours of the pituitary gland and are mainly considered as benign. The primary aim of this study was to research the effects of NFPA on genome instability in patients with non-functioning pituitary adenoma by using the cytokinesis-block micronucleus cytome (CBMN-cyt) assay and 8-hydroxy- 2’-deoxyguanosine (8-OHdG) assay. The second objective of this study was to assess whether there is a relationship between age, pituitary adenoma diameters, 8-OHDG levels, CBMN site assay parameters, and tumour aggressiveness.

Material and methods: The study was performed on 30 patients who had been diagnosed with NFPA and were admitted to the Department of Endocrinology and Metabolism, and 20 healthy subjects of similar age and sex.

Results: Micronucleus (MN), nucleoplasmic bridges (NPBs), nuclear bud (NBUD) frequencies, and apoptotic and necrotic cell frequencies in patients with NFPA were found to be significantly higher than in control subjects, and plasma 8-OHdG levels in patients with NFPA were statistically significantly lower than control subjects in this study.

Conclusions: It is believed that this is the first study to evaluate the aggressiveness of tumour with chromosome/oxidative DNA damage in patients with NFPA. However, further studies are needed in order to understand the cause of NFPA aggression and to evaluate these patients in terms of risk of cancer. 

Get Citation

Keywords

8-OHdG levels; DNA damage; micronucleus; non-functioning pituitary adenoma

About this article
Title

Chromosomal and oxidative DNA damage in non-functioning pituitary adenomas

Journal

Endokrynologia Polska

Issue

Vol 72, No 2 (2021)

Article type

Original paper

Pages

97-103

Published online

2020-12-08

DOI

10.5603/EP.a2020.0084

Pubmed

33295635

Bibliographic record

Endokrynologia Polska 2021;72(2):97-103.

Keywords

8-OHdG levels
DNA damage
micronucleus
non-functioning pituitary adenoma

Authors

Nazmiye Bitgen
Fahri Bayram
Zuhal Hamurcu
Figen Ozturk
Yasin Simsek
Gulden Baskol
Ali Kurtsoy
Hamiyet Donmez-Altuntas

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