open access

Vol 72, No 1 (2021)
Original paper
Submitted: 2020-04-20
Accepted: 2020-07-03
Published online: 2020-10-28
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Aberrant expression of semaphorin 6B affects cell phenotypes in thyroid carcinoma by activating the Notch signalling pathway

Xiu-Juan Lv1, Xin Chen1, Yan Wang1, Shan Yu1, Lin Pang1, Chao Huang2
·
Pubmed: 33125690
·
Endokrynol Pol 2021;72(1):29-36.
Affiliations
  1. Department of Geriatric Medicine, The Fourth People Hospital of Shenyang, Huanggu District, Shenyang, China
  2. Department of Oncology, The Fourth People Hospital of Shenyang, Huanggu District, Shenyang, China

open access

Vol 72, No 1 (2021)
Original Paper
Submitted: 2020-04-20
Accepted: 2020-07-03
Published online: 2020-10-28

Abstract

Introduction: Numerous semaphorins have been widely clarified to be involved in the development of multiple cancers. However, semaphorin 6B (SEMA6B) has not yet been extensively reported in cancers, especially in thyroid carcinoma.

Material and methods: Thyroid carcinoma RNA-Seq dataset from the TCGA database was used to assess the expression of SEMA6B in tissues, as well as its clinical significance. We adopted qRT-PCR and western blot analyses to measure the mRNA and protein expression of SEMA6B in thyroid carcinoma cells. The biological roles of SEMA6B in thyroid carcinoma cells were examined through cell counting kit 8, clone formation, and Transwell assays. Also, GSEA was used to identify the gene sets modulated by SEMA6B, which is further verified by western blot.

Results: According to the public dataset from the TCGA database, we found that the expression of SEMA6B was upregulated in thyroid carcinoma tissues compared to adjacent non-tumour tissues, and a high level of SEMA6B resulted in a poorer prognosis compared to the low-level SEMA6B group. Functional experiments showed that silencing SEMA6B suppressed the B-CPAP cells viability, invasiveness, and motility, whereas up-regulating SEMA6B in FTC-133 cells led to opposite outcomes. Furthermore, knockdown of SEMA6B in B-CPAP cells could significantly elevate the protein expression of NUMB and reduce the expression of NOTCH1, HES1, and Cyclin D1. Conversely, overexpression of SEMA6B in FTC-133 cells presented opposite results on the protein expression of these Notch signalling pathway-related markers.

Conclusions: Our findings demonstrated that SEMA6B exerts a tumourigenic effect in thyroid carcinoma partly by activating Notch signalling pathway, which provides a possible biomarker for the therapeutic intervention in thyroid carcinoma.

Abstract

Introduction: Numerous semaphorins have been widely clarified to be involved in the development of multiple cancers. However, semaphorin 6B (SEMA6B) has not yet been extensively reported in cancers, especially in thyroid carcinoma.

Material and methods: Thyroid carcinoma RNA-Seq dataset from the TCGA database was used to assess the expression of SEMA6B in tissues, as well as its clinical significance. We adopted qRT-PCR and western blot analyses to measure the mRNA and protein expression of SEMA6B in thyroid carcinoma cells. The biological roles of SEMA6B in thyroid carcinoma cells were examined through cell counting kit 8, clone formation, and Transwell assays. Also, GSEA was used to identify the gene sets modulated by SEMA6B, which is further verified by western blot.

Results: According to the public dataset from the TCGA database, we found that the expression of SEMA6B was upregulated in thyroid carcinoma tissues compared to adjacent non-tumour tissues, and a high level of SEMA6B resulted in a poorer prognosis compared to the low-level SEMA6B group. Functional experiments showed that silencing SEMA6B suppressed the B-CPAP cells viability, invasiveness, and motility, whereas up-regulating SEMA6B in FTC-133 cells led to opposite outcomes. Furthermore, knockdown of SEMA6B in B-CPAP cells could significantly elevate the protein expression of NUMB and reduce the expression of NOTCH1, HES1, and Cyclin D1. Conversely, overexpression of SEMA6B in FTC-133 cells presented opposite results on the protein expression of these Notch signalling pathway-related markers.

Conclusions: Our findings demonstrated that SEMA6B exerts a tumourigenic effect in thyroid carcinoma partly by activating Notch signalling pathway, which provides a possible biomarker for the therapeutic intervention in thyroid carcinoma.

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Keywords

semaphorin 6B; thyroid cancer; cell growth; invasion and migration; Notch signalling

About this article
Title

Aberrant expression of semaphorin 6B affects cell phenotypes in thyroid carcinoma by activating the Notch signalling pathway

Journal

Endokrynologia Polska

Issue

Vol 72, No 1 (2021)

Article type

Original paper

Pages

29-36

Published online

2020-10-28

Page views

1051

Article views/downloads

685

DOI

10.5603/EP.a2020.0072

Pubmed

33125690

Bibliographic record

Endokrynol Pol 2021;72(1):29-36.

Keywords

semaphorin 6B
thyroid cancer
cell growth
invasion and migration
Notch signalling

Authors

Xiu-Juan Lv
Xin Chen
Yan Wang
Shan Yu
Lin Pang
Chao Huang

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