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open access

Vol 69, No 3 (2018)
Original paper
Submitted: 2017-12-04
Accepted: 2018-02-01
Published online: 2018-04-12
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Adiponectin expression in visceral adiposity is an important determinant of insulin resistance in morbid obesity

Anca Elena Sirbu12, Laura Buburuzan3, Steliana Kevorkian3, Sorina Martin12, Carmen Barbu12, Catalin Copaescu14, Bogdan Smeu4, Simona Fica12
DOI: 10.5603/EP.a2018.0026
·
Pubmed: 29645064
·
Endokrynol Pol 2018;69(3):252-258.
Affiliations
  1. “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania
  2. Elias University Hospital, bd Marasti, 17, sector 1, Bucharest
  3. University of Bucharest, Department of Biochemistry and Molecular Biology, Bucharest, Romania
  4. Ponderas Hospital, Bucharest, Romania

open access

Vol 69, No 3 (2018)
Original Paper
Submitted: 2017-12-04
Accepted: 2018-02-01
Published online: 2018-04-12

Abstract

Introduction: Visceral adiposity is associated with decreased serum adiponectin levels, peripheral resistance to insulin and an increased risk of cardio-metabolic complications. However, the link between adiponectin expression in visceral adipose tissue (VAT), its serum levels and metabolic protection is controversial. The aim of this study was to investigate the relationship between the adiponectin gene expression in VAT and clinical and metabolic parameters in patients with severe obesity. Material and Methods: This is a cross-sectional study that included 51 severely obese patients (age 43.24±11.29 years, BMI 45.13±8.67 kg/m2), extensively evaluated clinically and biologically (metabolic tests, serum adiponectin measurements, HOMA-IR) before bariatric surgery. Omental adipose tissue was sampled during the intervention and the relative quantification of adiponectin gene expression was performed by real-time PCR, using beta-actin as reference gene. Results. Adiponectin mRNA in VAT was significantly higher in obese insulin-sensitive patients than in the rest of obese patients (p < 0.05) and negatively correlated with HOMA-IR (r =-0.354, p=0.016) and uric acid (r =-0.304, p=0.045). After adjustment for gender, TG/HDL ratio and uric acid, adiponectin expresion (β= -0.439, p=0.001), waist circumference (β=0.467, p=0.001) and serum adiponectin (β =-0.339, p=0.011) remained significantly associated with HOMA-IR, together explaining more than 50% of its variation. Conclusions. In severely obese patients, adiponectin gene expression in VAT is negatively correlated with serum levels of uric acid and is an independent determinant, together with anthropometric parameters of visceral obesity and serum adiponectin levels, of insulin resistance.

Abstract

Introduction: Visceral adiposity is associated with decreased serum adiponectin levels, peripheral resistance to insulin and an increased risk of cardio-metabolic complications. However, the link between adiponectin expression in visceral adipose tissue (VAT), its serum levels and metabolic protection is controversial. The aim of this study was to investigate the relationship between the adiponectin gene expression in VAT and clinical and metabolic parameters in patients with severe obesity. Material and Methods: This is a cross-sectional study that included 51 severely obese patients (age 43.24±11.29 years, BMI 45.13±8.67 kg/m2), extensively evaluated clinically and biologically (metabolic tests, serum adiponectin measurements, HOMA-IR) before bariatric surgery. Omental adipose tissue was sampled during the intervention and the relative quantification of adiponectin gene expression was performed by real-time PCR, using beta-actin as reference gene. Results. Adiponectin mRNA in VAT was significantly higher in obese insulin-sensitive patients than in the rest of obese patients (p < 0.05) and negatively correlated with HOMA-IR (r =-0.354, p=0.016) and uric acid (r =-0.304, p=0.045). After adjustment for gender, TG/HDL ratio and uric acid, adiponectin expresion (β= -0.439, p=0.001), waist circumference (β=0.467, p=0.001) and serum adiponectin (β =-0.339, p=0.011) remained significantly associated with HOMA-IR, together explaining more than 50% of its variation. Conclusions. In severely obese patients, adiponectin gene expression in VAT is negatively correlated with serum levels of uric acid and is an independent determinant, together with anthropometric parameters of visceral obesity and serum adiponectin levels, of insulin resistance.

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Keywords

adiponectin expression, visceral adipose tissue, HOMA-IR, insulin resistance, severe obesity

About this article
Title

Adiponectin expression in visceral adiposity is an important determinant of insulin resistance in morbid obesity

Journal

Endokrynologia Polska

Issue

Vol 69, No 3 (2018)

Article type

Original paper

Pages

252-258

Published online

2018-04-12

Page views

2197

Article views/downloads

1714

DOI

10.5603/EP.a2018.0026

Pubmed

29645064

Bibliographic record

Endokrynol Pol 2018;69(3):252-258.

Keywords

adiponectin expression
visceral adipose tissue
HOMA-IR
insulin resistance
severe obesity

Authors

Anca Elena Sirbu
Laura Buburuzan
Steliana Kevorkian
Sorina Martin
Carmen Barbu
Catalin Copaescu
Bogdan Smeu
Simona Fica

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