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Vol 69, No 2 (2018)
Original paper
Submitted: 2017-09-29
Accepted: 2017-12-05
Published online: 2018-01-12
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Markers of proliferation and invasiveness in somatotropinomas

Agata Baldys-Waligorska12, Iga Wierzbicka34, Grzegorz Sokolowski12, Dariusz Adamek56, Filip Golkowski12
DOI: 10.5603/EP.a2018.0001
·
Pubmed: 29334118
·
Endokrynol Pol 2018;69(2):182-189.
Affiliations
  1. Department of Endocrinology, Faculty of Medicine Jagiellonian University, Medical College, Mikolaja Kopernika 17, 31-501 Krakow, Poland
  2. Katedra i Klinika Endokrynologii, Uniwersytet Jagielloński, Collegium Medicum, Mikołaja Kopernika 17, 31-501 Kraków, Poland
  3. Chair of Gastroenterology, Hepatology and Infectious Diseases, Faculty of Medicine Jagiellonian University, Medical College, ul. Sniadeckich 5, 31-531 Krakow, Poland
  4. Katedra Gastroenterologii, Hepatologii i Chorób Zakaźnych, Uniwersytet Jagielloński, Collegium Medicum, ul. Śniadeckich 5, 31-531 Kraków, Poland
  5. Chair of Patomorphology, Faculty of Medicine Jagiellonian University, Medical College, Grzegórzecka 16, 31-531 Kraków, Poland
  6. Katedra Patomorfologii, Uniwersytet Jagielloński, Collegium Medicum, ul. Grzegórzecka 16, 31-531 Kraków, Poland

open access

Vol 69, No 2 (2018)
Original Paper
Submitted: 2017-09-29
Accepted: 2017-12-05
Published online: 2018-01-12

Abstract

Introduction In the search for markers of invasiveness of pituitary adenomas, we studied the expression of Ki-67 antigen, TOPO 2A (topoisomerase 2 alpha), AIP (Aryl Hydrocarbon Receptor-Interacting Protein) and VEGF (Vascular Endothelial Growth Factor) in somatotropinomas. Material and Methods We retrospectively studied a group of 31 patients who underwent pituitary tumour surgery. Expression of Ki-67, TOPO 2A, AIP and VEGF in surgical specimens was determined by immunohistochemistry. Relations between quantitatively determined markers and clinical symptoms, tumour features, and MR imaging, were analysed. Acromegaly was confirmed by hormonal tests in all patients studied. Local invasiveness (cavernous sinus penetration, optic chiasm compression or suprasellar extension) was observed in 18/31 patients (58,1%). Results Ki-67 was expressed in 77.4%, TOPO 2A in 87.1%, AIP in 83.8%, and VEGF in 87.1% of 31 cases of somatropinoma. Median values of Ki-67, TOPO 2A, AIP and cytoplasmic VEGF indices were 1.2% [IQR=2.2], 1.5% [IQR=1.6], 21.26% [IQR=20.1] and 20.4% [IQR=15.4], respectively. Ki-67, TOPO 2A, AIP and VEGF expression was not correlated with age nor with patient gender (p > 0.05). Only Ki-67 and TOPO 2A correlated with tumour size (for Ki-67: r=0.42, p=0.025; for TOPO 2A: r=0.53, p=0.003). Ki-67 and TOPO 2A levels were significantly higher in invasive compared to noninvasive somatropinomas (Ki67 mean values: 1.85±1.33% vs. 0.95±1.07%, p=0.024; TOPO 2A mean values: 2.19±1.63% vs. 1.45±1.23%, , p=0.011). Conclusions Ki-67, TOPO 2A, AIP and VEGF were expressed in over 70% of all somatotropinomas. Only Ki-67 and TOPO 2A expression correlated with tumour size and tumour invasiveness.

Abstract

Introduction In the search for markers of invasiveness of pituitary adenomas, we studied the expression of Ki-67 antigen, TOPO 2A (topoisomerase 2 alpha), AIP (Aryl Hydrocarbon Receptor-Interacting Protein) and VEGF (Vascular Endothelial Growth Factor) in somatotropinomas. Material and Methods We retrospectively studied a group of 31 patients who underwent pituitary tumour surgery. Expression of Ki-67, TOPO 2A, AIP and VEGF in surgical specimens was determined by immunohistochemistry. Relations between quantitatively determined markers and clinical symptoms, tumour features, and MR imaging, were analysed. Acromegaly was confirmed by hormonal tests in all patients studied. Local invasiveness (cavernous sinus penetration, optic chiasm compression or suprasellar extension) was observed in 18/31 patients (58,1%). Results Ki-67 was expressed in 77.4%, TOPO 2A in 87.1%, AIP in 83.8%, and VEGF in 87.1% of 31 cases of somatropinoma. Median values of Ki-67, TOPO 2A, AIP and cytoplasmic VEGF indices were 1.2% [IQR=2.2], 1.5% [IQR=1.6], 21.26% [IQR=20.1] and 20.4% [IQR=15.4], respectively. Ki-67, TOPO 2A, AIP and VEGF expression was not correlated with age nor with patient gender (p > 0.05). Only Ki-67 and TOPO 2A correlated with tumour size (for Ki-67: r=0.42, p=0.025; for TOPO 2A: r=0.53, p=0.003). Ki-67 and TOPO 2A levels were significantly higher in invasive compared to noninvasive somatropinomas (Ki67 mean values: 1.85±1.33% vs. 0.95±1.07%, p=0.024; TOPO 2A mean values: 2.19±1.63% vs. 1.45±1.23%, , p=0.011). Conclusions Ki-67, TOPO 2A, AIP and VEGF were expressed in over 70% of all somatotropinomas. Only Ki-67 and TOPO 2A expression correlated with tumour size and tumour invasiveness.

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Keywords

pituitary adenoma, markers of proliferation, Ki-67, TOPO 2A, AIP, VEGF

About this article
Title

Markers of proliferation and invasiveness in somatotropinomas

Journal

Endokrynologia Polska

Issue

Vol 69, No 2 (2018)

Article type

Original paper

Pages

182-189

Published online

2018-01-12

Page views

2920

Article views/downloads

1268

DOI

10.5603/EP.a2018.0001

Pubmed

29334118

Bibliographic record

Endokrynol Pol 2018;69(2):182-189.

Keywords

pituitary adenoma
markers of proliferation
Ki-67
TOPO 2A
AIP
VEGF

Authors

Agata Baldys-Waligorska
Iga Wierzbicka
Grzegorz Sokolowski
Dariusz Adamek
Filip Golkowski

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