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Vol 69, No 1 (2018)
Original paper
Submitted: 2017-07-01
Accepted: 2017-09-19
Published online: 2017-12-21
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A preliminary study on the expression and clinical value of platelet-derived growth factor BB, hypoxia inducible factor-1α and C-C motif chemokine receptor-2 in peripheral blood during the pathogenesis of Graves’ disease

Ying Liu1, Jinglan Tang1, Qiaohong Hu1, Kefeng Lu1, Chunjie Hou1
DOI: 10.5603/EP.a2018.0004
·
Pubmed: 29319128
·
Endokrynol Pol 2018;69(1):9-15.
Affiliations
  1. Master of Medicine. Department of ultrasonography, Zhejiang Provincial People’s Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, 310014, P R China, China

open access

Vol 69, No 1 (2018)
Original Paper
Submitted: 2017-07-01
Accepted: 2017-09-19
Published online: 2017-12-21

Abstract

Introduction: Platelet-derived growth factor BB (PDGF-BB) plays an important role in the development of GD (Graves’ disease). However, it is still unknown whether PDGF-BB is expressed in peripheral blood and whether the expression of PDGF-BB contributes to GD. We aim to study the expression of PDGF-BB, hypoxia inducible factor (HIF)-1α and C-C motif chemokine receptor (CCR)-2 in peripheral blood of patients with GD and explore its effect and potential mechanism in pathogenesis. Material and methods: 41 patients with GD (GD group) and forty-five healthy people (control group) were chosen. The concentration of PDGF-BB and HIF-1α in peripheral blood specimens were detected and compared between the two groups. The expression of CCR2 in macrophages in the peripheral blood specimens were examined using FCM (Flow Cytometry). Results: Both PDGF-BB and HIF-1α were expressed in human peripheral blood from the two groups. Compared with specimens from healthy people, there were statistically increased concentrations of PDGF-BB and HIF-1α in the GD group (P < 0.05). The proportion of CCR2-positive macrophages in peripheral blood in the GD group was significantly higher than that in the control group (P < 0.05). Conclusions: CCR2-positive macrophages may induce the expression of PDGF-BB through HIF-1α signal, and the high expression of PDGF-BB may be involved in the pathogenesis of GD.

Abstract

Introduction: Platelet-derived growth factor BB (PDGF-BB) plays an important role in the development of GD (Graves’ disease). However, it is still unknown whether PDGF-BB is expressed in peripheral blood and whether the expression of PDGF-BB contributes to GD. We aim to study the expression of PDGF-BB, hypoxia inducible factor (HIF)-1α and C-C motif chemokine receptor (CCR)-2 in peripheral blood of patients with GD and explore its effect and potential mechanism in pathogenesis. Material and methods: 41 patients with GD (GD group) and forty-five healthy people (control group) were chosen. The concentration of PDGF-BB and HIF-1α in peripheral blood specimens were detected and compared between the two groups. The expression of CCR2 in macrophages in the peripheral blood specimens were examined using FCM (Flow Cytometry). Results: Both PDGF-BB and HIF-1α were expressed in human peripheral blood from the two groups. Compared with specimens from healthy people, there were statistically increased concentrations of PDGF-BB and HIF-1α in the GD group (P < 0.05). The proportion of CCR2-positive macrophages in peripheral blood in the GD group was significantly higher than that in the control group (P < 0.05). Conclusions: CCR2-positive macrophages may induce the expression of PDGF-BB through HIF-1α signal, and the high expression of PDGF-BB may be involved in the pathogenesis of GD.

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Keywords

PDGF-BB, Graves’ disease, HIF-1α, macrophages, immunology

About this article
Title

A preliminary study on the expression and clinical value of platelet-derived growth factor BB, hypoxia inducible factor-1α and C-C motif chemokine receptor-2 in peripheral blood during the pathogenesis of Graves’ disease

Journal

Endokrynologia Polska

Issue

Vol 69, No 1 (2018)

Article type

Original paper

Pages

9-15

Published online

2017-12-21

Page views

1344

Article views/downloads

1085

DOI

10.5603/EP.a2018.0004

Pubmed

29319128

Bibliographic record

Endokrynol Pol 2018;69(1):9-15.

Keywords

PDGF-BB
Graves’ disease
HIF-1α
macrophages
immunology

Authors

Ying Liu
Jinglan Tang
Qiaohong Hu
Kefeng Lu
Chunjie Hou

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