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Probiotics for experimental obesity prevention: focus on strain dependence and viability of composition
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Abstract
Introduction: a comparative animal study of the efficacy of intermittent short-course administration of lyophilised single-, three-, and live multistrain probiotic on obesity.
Methods: We included 70 rats divided into seven groups (n = 10 in each). Rats of group I were intact. Newborn rats of groups II–VII were injected with monosodium glutamate (MSG) (4 mg/g). Rats of group II (MSG-obesity group) were untreated. The group III-V received lyophilised monoprobiotics B. animalis VKL, B. animalis VKB, and L. casei IMVB-7280, respectively. Group VI received the mix of these three probiotic strains. Group VII was treated with multiprobiotic “Symbiter”, which contains 14 live probiotic strains (Lactobacillus, Bifidobacterium, Propionibacterium, Acetobacter genera).
Results: Neonatal treatment with MSG caused stunted growth, which is why, despite the lack of weight gain dynamics and absence of significant food consumption rate and body weight changes at day 120, we noted the development of obesity in all MSG-obesity rats and in up to 20–70% after probiotic administration. Supplementation of probiotic composition, with preference to live strains, led to a significantly lower prevalence of obesity, and reduction of VAT weight and serum lipid levels as compared to single-strain probiotic. In our comparative single-strain analysis a trend towards more pronounced hypolipidaemic effect and VAT weight reduction was observed for lyophilised L. casei IMVB-7280 as compared to B. animalis VKL and VKB strains.
Conclusions: Multistrain formed mutualistic interactions in mixtures and therefore able to share with different metabolites, affect different receptors and produced various of biologically active compounds which synergistic overall effect greater than the sum of the single effects.
Abstract
Introduction: a comparative animal study of the efficacy of intermittent short-course administration of lyophilised single-, three-, and live multistrain probiotic on obesity.
Methods: We included 70 rats divided into seven groups (n = 10 in each). Rats of group I were intact. Newborn rats of groups II–VII were injected with monosodium glutamate (MSG) (4 mg/g). Rats of group II (MSG-obesity group) were untreated. The group III-V received lyophilised monoprobiotics B. animalis VKL, B. animalis VKB, and L. casei IMVB-7280, respectively. Group VI received the mix of these three probiotic strains. Group VII was treated with multiprobiotic “Symbiter”, which contains 14 live probiotic strains (Lactobacillus, Bifidobacterium, Propionibacterium, Acetobacter genera).
Results: Neonatal treatment with MSG caused stunted growth, which is why, despite the lack of weight gain dynamics and absence of significant food consumption rate and body weight changes at day 120, we noted the development of obesity in all MSG-obesity rats and in up to 20–70% after probiotic administration. Supplementation of probiotic composition, with preference to live strains, led to a significantly lower prevalence of obesity, and reduction of VAT weight and serum lipid levels as compared to single-strain probiotic. In our comparative single-strain analysis a trend towards more pronounced hypolipidaemic effect and VAT weight reduction was observed for lyophilised L. casei IMVB-7280 as compared to B. animalis VKL and VKB strains.
Conclusions: Multistrain formed mutualistic interactions in mixtures and therefore able to share with different metabolites, affect different receptors and produced various of biologically active compounds which synergistic overall effect greater than the sum of the single effects.
Keywords
obesity, lyophilised and alive probiotic strains, Lactobacillus, Bifidobacterium, multistrain probiotics
About this articleTitle
Probiotics for experimental obesity prevention: focus on strain dependence and viability of composition
Journal
Issue
Article type
Original paper
Pages
659-667
Published online
2017-09-22
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3193
Article views/downloads
2065
DOI
Pubmed
Bibliographic record
Endokrynol Pol 2017;68(6):659-667.
Keywords
obesity
lyophilised and alive probiotic strains
Lactobacillus
Bifidobacterium
multistrain probiotics
Authors
Nazarii Kobyliak
Tetyana Falalyeyeva
Tetyana Beregova
Mykola Spivak
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