open access

Vol 66, No 4 (2015)
Original papers
Published online: 2015-09-01
Submitted: 2014-10-02
Accepted: 2015-04-23
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Vitamin-D receptor gene polymorphisms (TaqI and ApaI) and circulating osteocalcin in type 2 diabetic patients and healthy subjects.

Edgar-Alfonso Rivera-Leon, Beatriz Palmeros-Sanchez, Iris-Monserrat Llamas-Covarrubias, Socorro Fernandez, Juan Armendariz-Borunda, Mercedes Gonzalez-Hita, Blanca-Estela Bastidas-Ramirez, Abraham Zepeda-Moreno, Sergio Sanchez-Enriquez
DOI: 10.5603/EP.2015.0042
·
Endokrynologia Polska 2015;66(4):329-333.

open access

Vol 66, No 4 (2015)
Original papers
Published online: 2015-09-01
Submitted: 2014-10-02
Accepted: 2015-04-23

Abstract

Introduction: Vitamin D receptor (VDR) is encoded by the VDR gene. Several studies have supported that this gene is associated with diabetes. Heterodimer VDR/RXR functions as an enhancer of the BGLAP gene and increases the basal transcription rate of osteocalcin (OC) during osteoblast differentiation. OC is a regulator of glucose metabolism in mice. Moreover, OC level is decreased in patients with type 2 diabetes (T2D). Although inversely correlated with serum glucose insulin and glycated haemoglobin, it is unclear whether OC reduction is caused by diabetes or plays a role in the pathogenesis and/or progression of the disease. In this study we analysed the association between TaqI and ApaI VDR gene polymorphisms and OC serum concentration in T2D subjects.

Material and methods: Patients underwent clinical and nutritional assessment. Genomic DNA was extracted from leucocytes using a standard salting-out procedure. The polymorphisms were genotyped by PCR-RFLP method. ELISA was used to measure OC and insulin concentrations.

Results: Association between TT genotype of TaqI polymorphism and low levels of OC was observed only in the population with overweight and obesity. No association between TaqI and ApaI polymorphisms and T2D was observed (p > 0.05). Furthermore, in T2D subjects, no correlation between ApaI and TaqI genotypes and age, sex, Body Mass Index (BMI), glucose, or OC was observed.

Conclusions: The TT genotype of TaqI VDR gene polymorphism was correlated with low levels of OC in overweight and obese subjects. However, TaqI and ApaI VDR gene polymorphisms were not associated with T2D. (Endokrynol Pol 2015; 66 (4): 329–333)

Abstract

Introduction: Vitamin D receptor (VDR) is encoded by the VDR gene. Several studies have supported that this gene is associated with diabetes. Heterodimer VDR/RXR functions as an enhancer of the BGLAP gene and increases the basal transcription rate of osteocalcin (OC) during osteoblast differentiation. OC is a regulator of glucose metabolism in mice. Moreover, OC level is decreased in patients with type 2 diabetes (T2D). Although inversely correlated with serum glucose insulin and glycated haemoglobin, it is unclear whether OC reduction is caused by diabetes or plays a role in the pathogenesis and/or progression of the disease. In this study we analysed the association between TaqI and ApaI VDR gene polymorphisms and OC serum concentration in T2D subjects.

Material and methods: Patients underwent clinical and nutritional assessment. Genomic DNA was extracted from leucocytes using a standard salting-out procedure. The polymorphisms were genotyped by PCR-RFLP method. ELISA was used to measure OC and insulin concentrations.

Results: Association between TT genotype of TaqI polymorphism and low levels of OC was observed only in the population with overweight and obesity. No association between TaqI and ApaI polymorphisms and T2D was observed (p > 0.05). Furthermore, in T2D subjects, no correlation between ApaI and TaqI genotypes and age, sex, Body Mass Index (BMI), glucose, or OC was observed.

Conclusions: The TT genotype of TaqI VDR gene polymorphism was correlated with low levels of OC in overweight and obese subjects. However, TaqI and ApaI VDR gene polymorphisms were not associated with T2D. (Endokrynol Pol 2015; 66 (4): 329–333)

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Keywords

VDR; osteocalcin; genotypes; polymorphisms; T2D

About this article
Title

Vitamin-D receptor gene polymorphisms (TaqI and ApaI) and circulating osteocalcin in type 2 diabetic patients and healthy subjects.

Journal

Endokrynologia Polska

Issue

Vol 66, No 4 (2015)

Pages

329-333

Published online

2015-09-01

DOI

10.5603/EP.2015.0042

Bibliographic record

Endokrynologia Polska 2015;66(4):329-333.

Keywords

VDR
osteocalcin
genotypes
polymorphisms
T2D

Authors

Edgar-Alfonso Rivera-Leon
Beatriz Palmeros-Sanchez
Iris-Monserrat Llamas-Covarrubias
Socorro Fernandez
Juan Armendariz-Borunda
Mercedes Gonzalez-Hita
Blanca-Estela Bastidas-Ramirez
Abraham Zepeda-Moreno
Sergio Sanchez-Enriquez

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