open access

Vol 65, No 6 (2014)
Original papers
Published online: 2014-12-31
Submitted: 2014-01-21
Accepted: 2014-03-12
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First one-step nucleic acid amplification testing in papillary thyroid cancer lymph nodes — a comparison with histopathology and real-time PCR

Krzysztof Kaczka, Wojciech Fendler, Maciej Borowiec, Wojciech Młynarski, Lech Pomorski
DOI: 10.5603/EP.2014.0059
·
Endokrynologia Polska 2014;65(6):422-430.

open access

Vol 65, No 6 (2014)
Original papers
Published online: 2014-12-31
Submitted: 2014-01-21
Accepted: 2014-03-12

Abstract

Introduction: The significance of lymph node metastases and the optimal extent of lymphadenectomy remain matters of controversy in papillary thyroid cancer. This study was designed to assess the feasibility and reliability of OSNA and real-time PCR for CK19 and TG mRNA in papillary thyroid cancer lymph nodes evaluation compared to standard histopathology.

Material and methods: Each of 92 randomised lymph nodes from 32 papillary thyroid cancer patients were divided into representative parts and assessed using the three studied methods.

Results: Eighteen (19.6%) lymph nodes from ten (31.3%) patients were positive according to histopathology. When the cut-off value distinguishing metastatic from non-metastatic lymph nodes in the OSNA assay was set at 250 copies per microlitre, the results were positive in 16 (17.4%) lymph nodes from 11 (34.4%) patients. Twenty three (25%) lymph nodes were tested positive in real-time PCR for TG mRNA. Real-time PCR for CK19 mRNA was positive in 18 (19.6%) lymph nodes from 13 (40.6%) patients. No statistically significant differences were noted between the diagnostic accuracy of either molecular method compared to the histopathological examination (p = 0.81). Overall, 20 positive molecular biology results were noted in patients with negative histopathology results. Conversely, in 18 lymph nodes, despite a metastasis finding in histopathology, at least one molecular test yielded a negative result.

Conclusions: It was revealed that OSNA is a reliable technique for the evaluation of lymph node metastases in papillary thyroid cancer. This method was shown to have equivalent accuracy to histopathology and real-time PCR. (Endokrynol Pol 2014; 65 (6): 422–430)

Abstract

Introduction: The significance of lymph node metastases and the optimal extent of lymphadenectomy remain matters of controversy in papillary thyroid cancer. This study was designed to assess the feasibility and reliability of OSNA and real-time PCR for CK19 and TG mRNA in papillary thyroid cancer lymph nodes evaluation compared to standard histopathology.

Material and methods: Each of 92 randomised lymph nodes from 32 papillary thyroid cancer patients were divided into representative parts and assessed using the three studied methods.

Results: Eighteen (19.6%) lymph nodes from ten (31.3%) patients were positive according to histopathology. When the cut-off value distinguishing metastatic from non-metastatic lymph nodes in the OSNA assay was set at 250 copies per microlitre, the results were positive in 16 (17.4%) lymph nodes from 11 (34.4%) patients. Twenty three (25%) lymph nodes were tested positive in real-time PCR for TG mRNA. Real-time PCR for CK19 mRNA was positive in 18 (19.6%) lymph nodes from 13 (40.6%) patients. No statistically significant differences were noted between the diagnostic accuracy of either molecular method compared to the histopathological examination (p = 0.81). Overall, 20 positive molecular biology results were noted in patients with negative histopathology results. Conversely, in 18 lymph nodes, despite a metastasis finding in histopathology, at least one molecular test yielded a negative result.

Conclusions: It was revealed that OSNA is a reliable technique for the evaluation of lymph node metastases in papillary thyroid cancer. This method was shown to have equivalent accuracy to histopathology and real-time PCR. (Endokrynol Pol 2014; 65 (6): 422–430)

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Keywords

OSNA; histopathology; real-time PCR; lymph nodes; papillary thyroid cancer

About this article
Title

First one-step nucleic acid amplification testing in papillary thyroid cancer lymph nodes — a comparison with histopathology and real-time PCR

Journal

Endokrynologia Polska

Issue

Vol 65, No 6 (2014)

Pages

422-430

Published online

2014-12-31

DOI

10.5603/EP.2014.0059

Bibliographic record

Endokrynologia Polska 2014;65(6):422-430.

Keywords

OSNA
histopathology
real-time PCR
lymph nodes
papillary thyroid cancer

Authors

Krzysztof Kaczka
Wojciech Fendler
Maciej Borowiec
Wojciech Młynarski
Lech Pomorski

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