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Research paper
Published online: 2021-05-24
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The relation between IL-10 gene (-1082G/A) and VEGF gene 936 C/T polymorphism and diabetic polyneuropathy in a cohort of Egyptian patients with type 2 diabetes

Salma Alaa Eldin Imbaby, Mai Badrah, Yousra Hisham Abdel-Fattah
DOI: 10.5603/DK.a2021.0036

open access

Ahead of print
ORIGINAL ARTICLES
Published online: 2021-05-24

Abstract

Background: Polymorphisms have been described to correlate with T2DM and its complications including diabetic polyneuropathy (DPN). The aim of this study was to investigate the relation between interleukin (IL)-10-1082 G/A and vascular endothelial growth factor (VEGF)-936 C/T polymorphism and DPN in type 2 diabetes mellitus (T2DM) patients. Methods: This cross-sectional study included 50 T2DM patients and 40 controls. Clinical and electrophysiological assessments for DPN, fasting blood glucose level (FBS) and glycosylated haemoglobin (Hb A1C) were recorded. VEGF-936 C/T polymorphism was carried out by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Genotyping for IL-10 promotor gene (-1082 G/A) polymorphism was performed using Real-time PCR. Results: Sixty percent of patients had confirmed DPN, while none were considered normal. IL-10 (genotype -1082G/G) was statistically higher among controls compared to patients (p=0.008). VEGF-936-CT genotype was statistically higher among patients compared to controls (p=0.033), but there was no significant relation between IL-10-1082 G/A or VEGF-936 C/T polymorphism genotypes and DPN. IL-10 (genotype -1082A/G) had higher HbA1C levels (p=0.041) and a lower albumin/creatinine ratio, while IL-10 (genotype -1082G/G) had a higher albumin/creatinine ratio (p=0.024). DPN had a significant correlation with duration of diabetes, FBS and HbA1C. Conclusion: The VEGF-936 C/T genotype may be associated with T2DM, while the IL-10 (genotype -1082G/G) may be less likely to be associated with it; however there was no association between VEGF-936 or IL-10-1082 genotypes and DPN.

Abstract

Background: Polymorphisms have been described to correlate with T2DM and its complications including diabetic polyneuropathy (DPN). The aim of this study was to investigate the relation between interleukin (IL)-10-1082 G/A and vascular endothelial growth factor (VEGF)-936 C/T polymorphism and DPN in type 2 diabetes mellitus (T2DM) patients. Methods: This cross-sectional study included 50 T2DM patients and 40 controls. Clinical and electrophysiological assessments for DPN, fasting blood glucose level (FBS) and glycosylated haemoglobin (Hb A1C) were recorded. VEGF-936 C/T polymorphism was carried out by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Genotyping for IL-10 promotor gene (-1082 G/A) polymorphism was performed using Real-time PCR. Results: Sixty percent of patients had confirmed DPN, while none were considered normal. IL-10 (genotype -1082G/G) was statistically higher among controls compared to patients (p=0.008). VEGF-936-CT genotype was statistically higher among patients compared to controls (p=0.033), but there was no significant relation between IL-10-1082 G/A or VEGF-936 C/T polymorphism genotypes and DPN. IL-10 (genotype -1082A/G) had higher HbA1C levels (p=0.041) and a lower albumin/creatinine ratio, while IL-10 (genotype -1082G/G) had a higher albumin/creatinine ratio (p=0.024). DPN had a significant correlation with duration of diabetes, FBS and HbA1C. Conclusion: The VEGF-936 C/T genotype may be associated with T2DM, while the IL-10 (genotype -1082G/G) may be less likely to be associated with it; however there was no association between VEGF-936 or IL-10-1082 genotypes and DPN.

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Keywords

type 2 diabetes mellitus; polymorphism; interleukin-10; vascular endothelial growth factor; diabetic polyneuropathy; total neuropathy score.

About this article
Title

The relation between IL-10 gene (-1082G/A) and VEGF gene 936 C/T polymorphism and diabetic polyneuropathy in a cohort of Egyptian patients with type 2 diabetes

Journal

Clinical Diabetology

Issue

Ahead of print

Article type

Research paper

Published online

2021-05-24

DOI

10.5603/DK.a2021.0036

Keywords

type 2 diabetes mellitus
polymorphism
interleukin-10
vascular endothelial growth factor
diabetic polyneuropathy
total neuropathy score.

Authors

Salma Alaa Eldin Imbaby
Mai Badrah
Yousra Hisham Abdel-Fattah

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