open access

Vol 9, No 5 (2020)
Research paper
Published online: 2020-09-18
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Expression of Notch 2 and ABCC8 genes in patients with type 2 diabetes mellitus and their association with diabetic kidney disease

Yehia Ghanem, Azza Ismail, Rania Elsharkawy, Reem Fathalla, Amr El Feky
DOI: 10.5603/DK.2020.0037
·
Clinical Diabetology 2020;9(5):306-312.

open access

Vol 9, No 5 (2020)
ORIGINAL ARTICLES
Published online: 2020-09-18

Abstract

Background. The incidence of type 2 diabetes mellitus (T2DM) has increased over the past years and early identification and management of its complications especially diabetic kidney disease (DKD) is of great importance. T2DM and DKD are of multifactorial etiology with contribution of genetic and environmental factors. We aimed to study the expression of ABCC8 and Notch 2 genes in patients with T2DM and to find their association with DKD. Methods. The present work was carried on 80 patients with T2DM (40 with DKD and 40 without DKD) and 40 healthy subjects as a control group. Real time polymerase chain reaction was used to assess gene expression. Results. Altered expression of ABCC8 and Notch 2 genes were found in patients with T2DM compared to control group. ABCC8 expression had significant positive correlation with HbA1c while Notch 2 expression had significant positive correlation with fasting plasma glucose and HbA1c. Notch 2 expression was significantly higher in patients with DKD compared to those without DKD. Multivariate regression analysis showed that Notch 2 expression had independent relation with increased urinary albumin excretion and reduced estimated glomerular filtration rate. ABCC8 gene expression did not show significant difference between diabetic patients with DKD compared to those without DKD. Conclusion. Increased expression of ABCC8 and Notch 2 genes may play a role in pathogenesis of T2DM. Overexpression of Notch 2 gene may have a role in the development of albuminuria and DKD in patients with T2DM which may represent a possible diagnostic tool and a possible therapeutic target.

Abstract

Background. The incidence of type 2 diabetes mellitus (T2DM) has increased over the past years and early identification and management of its complications especially diabetic kidney disease (DKD) is of great importance. T2DM and DKD are of multifactorial etiology with contribution of genetic and environmental factors. We aimed to study the expression of ABCC8 and Notch 2 genes in patients with T2DM and to find their association with DKD. Methods. The present work was carried on 80 patients with T2DM (40 with DKD and 40 without DKD) and 40 healthy subjects as a control group. Real time polymerase chain reaction was used to assess gene expression. Results. Altered expression of ABCC8 and Notch 2 genes were found in patients with T2DM compared to control group. ABCC8 expression had significant positive correlation with HbA1c while Notch 2 expression had significant positive correlation with fasting plasma glucose and HbA1c. Notch 2 expression was significantly higher in patients with DKD compared to those without DKD. Multivariate regression analysis showed that Notch 2 expression had independent relation with increased urinary albumin excretion and reduced estimated glomerular filtration rate. ABCC8 gene expression did not show significant difference between diabetic patients with DKD compared to those without DKD. Conclusion. Increased expression of ABCC8 and Notch 2 genes may play a role in pathogenesis of T2DM. Overexpression of Notch 2 gene may have a role in the development of albuminuria and DKD in patients with T2DM which may represent a possible diagnostic tool and a possible therapeutic target.

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Keywords

type 2 diabetes, ABCC8, Notch 2, diabetic kidney disease, gene expression

About this article
Title

Expression of Notch 2 and ABCC8 genes in patients with type 2 diabetes mellitus and their association with diabetic kidney disease

Journal

Clinical Diabetology

Issue

Vol 9, No 5 (2020)

Article type

Research paper

Pages

306-312

Published online

2020-09-18

DOI

10.5603/DK.2020.0037

Bibliographic record

Clinical Diabetology 2020;9(5):306-312.

Keywords

type 2 diabetes
ABCC8
Notch 2
diabetic kidney disease
gene expression

Authors

Yehia Ghanem
Azza Ismail
Rania Elsharkawy
Reem Fathalla
Amr El Feky

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