Vol 30, No 5 (2023)
Technology Note
Published online: 2023-09-12

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A novel noninvasive method for estimating right ventricular systolic pressure in rodents with pulmonary artery banding

Baktybek Kojonazarov12, Norbert Weissmann2, Werner Seeger123, Ralph Theo Schermuly2
Pubmed: 37772355
Cardiol J 2023;30(5):849-851.

Abstract

Not available

clinicAL CARDIOLOGY

TECHNOLOGY NOTE

Cardiology Journal

2023, Vol. 30, No. 5, 849–851

DOI: 10.5603/cj.93650

Copyright © 2023 Via Medica

ISSN 1897–5593

eISSN 1898–018X

A novel noninvasive method for estimating right ventricular systolic pressure in rodents with pulmonary artery banding

Baktybek Kojonazarov12Norbert Weissmann2Werner Seeger123Ralph Theo Schermuly2
1Institute for Lung Health (ILH), Giessen, Germany
2Universities of Giessen and Marburg Lung Center (UGMLC), German Center for Lung Research (DZL), Excellence Cluster Cardio-Pulmonary Institute (CPI), Giessen, Germany
3Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany

Address for correspondence: Baktybek Kojonazarov, MD, PhD, Institute for Lung Health (ILH), German Center for Lung Research (DZL), Cardio-Pulmonary Institute (CPI), Aulweg 130, 35392 Giessen, Germany, tel: +49 641 99 46802, fax: +49 641 99 46829, e-mail: baktybek.kojonazarov@innere.med.uni-giessen.de

Received: 12.01.2023 Accepted: 9.08.2023 Early publication date: 12.09.2023

This article is available in open access under Creative Common Attribution-Non-Commercial-No Derivatives 4.0 International (CC BY-NC-ND 4.0) license, allowing to download articles and share them with others as long as they credit the authors and the publisher, but without permission to change them in any way or use them commercially.

Pulmonary arterial hypertension (PAH) is a devastating disease characterized by remodeling of small pulmonary arteries, which causes increases in right ventricular (RV) afterload, RV hypertrophy, failure and death [1]. Pre-clinical disease models characterized by PAH-associated RV remodeling are currently utilized in proof-of-concept, dose-finding and efficacy studies to characterize novel RV-focused therapy. In these models it remains challenging to dissect afterload-dependent and -independent effects of potential therapeutics. Therefore, an animal model that exclusively affects the RV independently from changes in the pulmonary vasculature was established. This pulmonary artery banding (PAB) model allows one studies to assess direct treatment effects on the RV myocardium and to define mechanisms that underlie the transition from RV hypertrophy towards RV failure [2, 3]. PAB is a surgical technique historically used to limit pulmonary blood flow and treat pulmonary over-circulation caused by large left-to-right shunts in children with congenital heart defects. PAB in rodents creates a partial stenosis of the pulmonary artery (PA), leading to a constant elevation of afterload and resistance in the RV, similar to the effects of pulmonary valve stenosis in humans.

To define the pressure overload in rodents, RV systolic pressure (RVSP) derived from heart catheterization has emerged as “gold standard”; however, this procedure is terminal and thereby precludes longitudinal data acquisition. Therefore, reliable and clinically relevant non-invasively--derived indices may provide a valuable tool to monitor disease progression in pre-clinical studies. Echocardiography may provide a platform to assess such indices, as it’s noninvasive and used to characterize cardiac function in a longitudinal fashion [4].

In PAH patients’, echocardiography enables estimation of PA systolic pressure by measuring the tricuspid regurgitation jet (TRJ) pressure gradient (TRPG) calculated from Doppler-derived TRJ velocity using a simplified Bernoulli equation: TRPG = 4 × (TRJ)2 + right atrial (RA) pressure, estimated from vena cava respiratory variation. This method has demonstrated significant correlation between non-invasive Doppler and invasive catheter measurements as an accurate prediction of PAP [5]. While TRJ is challenging to detect in rodents, peak pressure gradient (PPG) measurements across the PA constriction site are routinely assessed in animals subjected to PAB. Using these measurements, herein provided is a novel method to estimate RVSP by echocardiography in PAB mice. Specifically, PPG was combined with E/E’ data to noninvasively determine RVSP. It has been shown that E/E’ ratio positively correlates with RA pressure [6, 7]. Although several formulas have been proposed to measure systolic or mean PAP in patients with pulmonary hypertension [8], there is a lack of reports describing the measurement of RVSP using pressure gradient (PG) and E/E’ ratio.

All experiments were performed according to institutional guidelines complying with national and international regulations and approved by local animal welfare authorities (RP Gießen). Male C57BL/6J mice aged 8–10 weeks (Charles River, Germany) were subjected to sham or PAB (n = 8–14 per group) surgery as described [3]. Three weeks post-surgery, echocardiography was performed using a Vevo2100 (Visualsonics, Canada) followed by invasive hemodynamic RVSP measurements. The PPG velocity was assessed by pulsed-wave Doppler and calculated using Bernoulli’s equation: PPG = 4 × PG2. Tricuspid inflow was recorded from the apical 4-chamber view by pulsed-wave Doppler to measure early (E) diastolic peak velocity. Tissue Doppler imaging was used to measure early (E’) diastolic velocity at the lateral corner of the tricuspid annulus (Fig. 1A). Noninvasive RVSP was determined as RVSP = 4(PG)2 + E/E’. Data were examined using Spearman correlation statistical analyses in Prism 8 software (GraphPad Software, La Jolla, CA).

Figure 1. A. Representative images demonstrating measurement of the pressure gradient via pulmonary artery constriction by pulsed wave Doppler, E’ and E peak velocity measured by tissue Doppler imaging and subsequent pulsed wave Doppler; B. Correlation between invasive right ventricular systolic pressure (RVSP) measurement and echo-Doppler-derived RVSP as a peak pressure gradient (PPG) across a pulmonary artery constriction plus E’ to E ratio.

It was previously described that PAB mice developed RV systolic and diastolic dysfunction [3]. In this study, RV catheterization showed increased RVSP to 44.5 ± 7.4 mmHg in PAB mice compared to sham animals 24.2 ± 3.5 mmHg (p = 0.0001). Echocardiography demonstrated that the E/E’ ratio increased in PAB mice to 26.3 ± 5.7 compared to 18.8 ± 4.3 in sham mice (p = 0.004). The PPG measured by echocardiography was 17.8 ± 6.3 mmHg in PAB mice. Interestingly, echo-derived PPG correlated with invasively measured RVSP (0.59, p = 0.02), while E/E’ ratio did not (0.30, p = 0.28). In the next step, RVSP calculations were derived from the echocardiography and was compared with invasive RV catheterization. In the present study, it was demonstrated that echocardiographic-derived RVSP closely correlates with invasively derived RVSP (r = 0.7978, p = 0.001, Fig. 1B) in a mouse model of PAB.

In the current study, a formula was introduced specifically designed to measure RVSP in the PAB model. This enabled the monitoring of RVSP in PAB mice at different stages of disease progression, providing a unique opportunity to investigate and monitor the effects of treatment on the RV. Therefore, the present study provides a valuable contribution to the current understanding of RV pathophysiology in PAB. Herein, a proposed formula for measuring RVSP could be a promising approach in clinical practice, especially in patients with congenital pulmonary valve stenosis and/or after palliative PAB surgery. This noninvasive method would enable clinicians to monitor disease progression in these patient populations, which could have significant clinical implications. Therefore, further research in this direction is warranted.

Acknowledgments

We acknowledge the excellent technical support and assistance in manuscript preparation provided by Christina Vroom and Mario Boehm in this study.

Funding

Institute for Lung Health (ILH), Excellence Cluster Cardio-Pulmonary Institute (CPI), and the German Research Foundation (DFG), Projektnummer 268555672, SFB 1213, Projects CP02.

Conflict of interest: None declared

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