Background: Acute myocardial infarction (AMI) is the leading cause of death for patients with
cardiovascular disease (CVD). Although researchers have made substantial efforts to elucidate its
pathogenesis, the molecular mechanisms underlying AMI remain unknown. The aim of this study was
to use proteomics to identify differentially expressed proteins (DEPs) and the possible biological functions
and metabolic pathways related to coronary blood microparticles (MPs) in patients with AMI and
stable coronary artery disease (SCAD); this study will allow for the identification of individuals at risk
of acute thrombosis.
Methods: The study was performed on 5 AMI patients and 5 SCAD patients. DEPs were identified,
and Gene Ontology (GO) enrichment and KEGG pathway enrichment analyzes were performed to
determine the relative abundance and biological function of the significant DEPs that were identified
in the present study.
Results: The current analysis identified 198 DEPs in the coronary blood of AMI patients and SCAD
patients, including 85 proteins that were significantly upregulated and 113 proteins that were significantly
downregulated. GO enrichment analysis demonstrated that GDP binding and GTP binding
were enriched in molecular function. Similarly, KEGG pathway enrichment analysis revealed that
the identified proteins were involved in pantothenate and coenzyme A biosynthesis, starch and sucrose
metabolism, and the AMPK signalling pathway.
Conclusions: The proteome of coronary MPs differs between patients with AMI and patients with
SCAD. In summary, the GO terms and KEGG pathways enriched by the DEPs may reflect the possible
molecular mechanisms underlying the pathogenesis of acute thrombosis in patients with AMI.