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Fast and simple method of morphine extraction from the blood
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Abstract
Background/Material and methods. In this study we evaluated the efficiency of morphine extraction from the whole blood by liquid-liquid methods using three types of organic solvents.
Results. The highest average recovery rate was observed for the 9:1 mixture of isopropanol and chloroform (92.5%). Lower, but still satisfactory, recovery rate was observed for acetonitril (72.7%) and dichloromethane (67.1%).
Conclusions. Isolation of morphine from the whole blood by simple solvent extraction with isopropanol-chloroform mixture is fast, efficient and easily accessible for each laboratory.
Abstract
Background/Material and methods. In this study we evaluated the efficiency of morphine extraction from the whole blood by liquid-liquid methods using three types of organic solvents.
Results. The highest average recovery rate was observed for the 9:1 mixture of isopropanol and chloroform (92.5%). Lower, but still satisfactory, recovery rate was observed for acetonitril (72.7%) and dichloromethane (67.1%).
Conclusions. Isolation of morphine from the whole blood by simple solvent extraction with isopropanol-chloroform mixture is fast, efficient and easily accessible for each laboratory.
Keywords
morphine; whole blood; extraction recovery rate
About this articleTitle
Fast and simple method of morphine extraction from the blood
Journal
Advances in Palliative Medicine
Issue
Vol 5, No 4 (2006): Polish Palliative Medicine
Pages
173-176
Published online
2007-01-15
Page views
708
Article views/downloads
2422
Bibliographic record
Advances in Palliative Medicine 2006;5(4):173-176.
Keywords
morphine
whole blood
extraction recovery rate
Authors
Małgorzata Krajnik
Marzena Sykutera
Ewa Pufal