open access

Vol 48, No 4 (2017)
Prace oryginalne / Original research articles
Published online: 2017-10-01
Submitted: 2017-03-10
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Vitamin C as a modulator of oxidative stress in erythrocytes of stored blood

Ravikumar Soumya1, Rajashekharaiah Vani
DOI: 10.1016/j.achaem.2017.08.005
·
Acta Haematol Pol 2017;48(4):350-356.
Affiliations
  1. Department of Biotechnology, Centre for Post Graduate Studies, Jain University, #18/3, 9th Main, 3rd Block, Jayanagar, Bangalore 560011, India

open access

Vol 48, No 4 (2017)
Prace oryginalne / Original research articles
Published online: 2017-10-01
Submitted: 2017-03-10

Abstract

Aim

To determine the effects of Vitamin C (VC-ascorbic acid) as an additive on erythrocytes of stored blood.

Background

Oxidative stress (OS) plays a major role in the formation of storage lesion of erythrocytes. Antioxidants, such as VC could be beneficial in combating oxidative damage during storage.

Materials and methods

Blood obtained from male Wistar rats was stored at 4°C in anticoagulant solution citrate-phosphate-dextrose-adenine solution. Blood samples were divided into 3 groups – (i) Controls, (ii) VC 10 (VC at a concentration of 10mM), (iii) VC 30 (VC at a concentration of 30mM). Markers of OS in erythrocytes such as – hemoglobin, superoxides, antioxidant enzymes (superoxide dismutase; SOD, catalase and glutathione peroxidase), hemolysis, lipid peroxidation products (conjugate dienes and malondialdehyde), protein oxidation products and ascorbic acid were determined on days 0, 10 and 15 of storage.

Results

Addition of ascorbic acid to the storage solution contributed to the protection of erythrocytes from oxidative damage. Ascorbic acid at a concentration of 30mM decreased SOD levels and increased protein sulfhydryls (P-SH) levels on day 15 showing that higher concentration supplemented the inherent antioxidant defense system of erythrocytes during blood storage.

Conclusion

VC proved to be effective in combating OS during blood storage. However, further exploration of antioxidants as additives and the erythrocyte storage lesion would result in better management of blood storage.

Abstract

Aim

To determine the effects of Vitamin C (VC-ascorbic acid) as an additive on erythrocytes of stored blood.

Background

Oxidative stress (OS) plays a major role in the formation of storage lesion of erythrocytes. Antioxidants, such as VC could be beneficial in combating oxidative damage during storage.

Materials and methods

Blood obtained from male Wistar rats was stored at 4°C in anticoagulant solution citrate-phosphate-dextrose-adenine solution. Blood samples were divided into 3 groups – (i) Controls, (ii) VC 10 (VC at a concentration of 10mM), (iii) VC 30 (VC at a concentration of 30mM). Markers of OS in erythrocytes such as – hemoglobin, superoxides, antioxidant enzymes (superoxide dismutase; SOD, catalase and glutathione peroxidase), hemolysis, lipid peroxidation products (conjugate dienes and malondialdehyde), protein oxidation products and ascorbic acid were determined on days 0, 10 and 15 of storage.

Results

Addition of ascorbic acid to the storage solution contributed to the protection of erythrocytes from oxidative damage. Ascorbic acid at a concentration of 30mM decreased SOD levels and increased protein sulfhydryls (P-SH) levels on day 15 showing that higher concentration supplemented the inherent antioxidant defense system of erythrocytes during blood storage.

Conclusion

VC proved to be effective in combating OS during blood storage. However, further exploration of antioxidants as additives and the erythrocyte storage lesion would result in better management of blood storage.

Get Citation

Keywords

Ascorbic acid; Erythrocyte; Blood storage; Lipid peroxidation; Protein oxidation

About this article
Title

Vitamin C as a modulator of oxidative stress in erythrocytes of stored blood

Journal

Acta Haematologica Polonica

Issue

Vol 48, No 4 (2017)

Pages

350-356

Published online

2017-10-01

DOI

10.1016/j.achaem.2017.08.005

Bibliographic record

Acta Haematol Pol 2017;48(4):350-356.

Keywords

Ascorbic acid
Erythrocyte
Blood storage
Lipid peroxidation
Protein oxidation

Authors

Ravikumar Soumya
Rajashekharaiah Vani

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