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Vitamin C as a modulator of oxidative stress in erythrocytes of stored blood


- Department of Biotechnology, Centre for Post Graduate Studies, Jain University, #18/3, 9th Main, 3rd Block, Jayanagar, Bangalore 560011, India
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Abstract
Aim
To determine the effects of Vitamin C (VC-ascorbic acid) as an additive on erythrocytes of stored blood.
Background
Oxidative stress (OS) plays a major role in the formation of storage lesion of erythrocytes. Antioxidants, such as VC could be beneficial in combating oxidative damage during storage.
Materials and methods
Blood obtained from male Wistar rats was stored at 4°C in anticoagulant solution citrate-phosphate-dextrose-adenine solution. Blood samples were divided into 3 groups – (i) Controls, (ii) VC 10 (VC at a concentration of 10mM), (iii) VC 30 (VC at a concentration of 30mM). Markers of OS in erythrocytes such as – hemoglobin, superoxides, antioxidant enzymes (superoxide dismutase; SOD, catalase and glutathione peroxidase), hemolysis, lipid peroxidation products (conjugate dienes and malondialdehyde), protein oxidation products and ascorbic acid were determined on days 0, 10 and 15 of storage.
Results
Addition of ascorbic acid to the storage solution contributed to the protection of erythrocytes from oxidative damage. Ascorbic acid at a concentration of 30mM decreased SOD levels and increased protein sulfhydryls (P-SH) levels on day 15 showing that higher concentration supplemented the inherent antioxidant defense system of erythrocytes during blood storage.
Conclusion
VC proved to be effective in combating OS during blood storage. However, further exploration of antioxidants as additives and the erythrocyte storage lesion would result in better management of blood storage.
Abstract
Aim
To determine the effects of Vitamin C (VC-ascorbic acid) as an additive on erythrocytes of stored blood.
Background
Oxidative stress (OS) plays a major role in the formation of storage lesion of erythrocytes. Antioxidants, such as VC could be beneficial in combating oxidative damage during storage.
Materials and methods
Blood obtained from male Wistar rats was stored at 4°C in anticoagulant solution citrate-phosphate-dextrose-adenine solution. Blood samples were divided into 3 groups – (i) Controls, (ii) VC 10 (VC at a concentration of 10mM), (iii) VC 30 (VC at a concentration of 30mM). Markers of OS in erythrocytes such as – hemoglobin, superoxides, antioxidant enzymes (superoxide dismutase; SOD, catalase and glutathione peroxidase), hemolysis, lipid peroxidation products (conjugate dienes and malondialdehyde), protein oxidation products and ascorbic acid were determined on days 0, 10 and 15 of storage.
Results
Addition of ascorbic acid to the storage solution contributed to the protection of erythrocytes from oxidative damage. Ascorbic acid at a concentration of 30mM decreased SOD levels and increased protein sulfhydryls (P-SH) levels on day 15 showing that higher concentration supplemented the inherent antioxidant defense system of erythrocytes during blood storage.
Conclusion
VC proved to be effective in combating OS during blood storage. However, further exploration of antioxidants as additives and the erythrocyte storage lesion would result in better management of blood storage.
Keywords
Ascorbic acid; Erythrocyte; Blood storage; Lipid peroxidation; Protein oxidation


Title
Vitamin C as a modulator of oxidative stress in erythrocytes of stored blood
Journal
Issue
Pages
350-356
Published online
2017-10-01
Page views
133
Article views/downloads
190
DOI
10.1016/j.achaem.2017.08.005
Bibliographic record
Acta Haematol Pol 2017;48(4):350-356.
Keywords
Ascorbic acid
Erythrocyte
Blood storage
Lipid peroxidation
Protein oxidation
Authors
Ravikumar Soumya
Rajashekharaiah Vani