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Methods for detection of microparticles derived from blood and endothelial cells
Affiliations- John Paul II Regional Blood Donation and Transfusion Center in Słupsk, 21 Szarych Szeregów St., 76-200 Słupsk, Poland
- Regional Blood Donation and Transfusion Center in Białystok, 23 Marii Skłodowskiej-Curie St., 15-950 Białystok, Poland
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Abstract
Microparticles (MP-microparticles) – cell membrane vesicles 0.1–1μm diameter, released in response to activation or apoptosis, both in physiological and pathological conditions. They reveal a wide spectrum of biological activities, express cell surface antigens characteristic for cells of their origin. In this article reviewed quantitative and qualitative methods for detection of microparticles, presents pre-analytical conditions as an potential source of variability in the analysis of MP. In conclusion there are several methods for detection of microparticles but they are not standarized. Methods of microparticles detection need to be standarized to be clinically relevant.
Abstract
Microparticles (MP-microparticles) – cell membrane vesicles 0.1–1μm diameter, released in response to activation or apoptosis, both in physiological and pathological conditions. They reveal a wide spectrum of biological activities, express cell surface antigens characteristic for cells of their origin. In this article reviewed quantitative and qualitative methods for detection of microparticles, presents pre-analytical conditions as an potential source of variability in the analysis of MP. In conclusion there are several methods for detection of microparticles but they are not standarized. Methods of microparticles detection need to be standarized to be clinically relevant.
Keywords
Microparticles; Methods of microparticles detection; Standarization; Pre-analytics
About this articleTitle
Methods for detection of microparticles derived from blood and endothelial cells
Journal
Issue
Pages
316-329
Published online
2017-10-01
Page views
110
Article views/downloads
231
DOI
10.1016/j.achaem.2017.03.003
Bibliographic record
Acta Haematol Pol 2017;48(4):316-329.
Keywords
Microparticles
Methods of microparticles detection
Standarization
Pre-analytics
Authors
Małgorzata Gradziuk
Piotr Radziwon