open access

Vol 48, No 2 (2017)
Prace poglądowe / Reviews
Published online: 2017-04-01
Submitted: 2016-09-12
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Methods for determination of fetal hemoglobin

Elżbieta Górska1, Anna Lemańska2, Katarzyna Popko1
DOI: 10.1016/j.achaem.2016.12.001
·
Acta Haematol Pol 2017;48(2):112-118.
Affiliations
  1. Zakład Diagnostyki Laboratoryjnej i Immunologii Klinicznej Wieku Rozwojowego, Warszawski Uniwersytet Medyczny, Warszawa, Polska
  2. Studenckie Koło Naukowe przy Zakładzie Diagnostyki Laboratoryjnej i Immunologii Klinicznej Wieku Rozwojowego, Warszawski Uniwersytet Medyczny, Kierownik: Prof dr hab. n. med. Urszula Demkow, Warszawa, Polska

open access

Vol 48, No 2 (2017)
Prace poglądowe / Reviews
Published online: 2017-04-01
Submitted: 2016-09-12

Abstract

The identification of the new form of hemoglobin in fetus red blood cells (HbF), different from adult hemoglobin (HbA), was made over one hundred years ago. Since this time, various methods of fetal hemoglobin measurement have been designed. Most of them are based on the different biochemical characteristics of HbF. Fetal hemoglobin's affinity for oxygen is substantially greater than that of adult hemoglobin. The first techniques for the determination of fetal hemoglobin were based on its resistance to denaturation by alkaline solutions. Currently, the measurement of hemoglobin F is an important part of diagnosis of sickle cell disease, thalassemia, hereditary persistence of fetal hemoglobin, and fetomaternal hemorrhage. Amongst the most commonly used and clinically important methods, high-performance liquid chromatography, capillary electrophoresis, isoelectric focusing, capillary isoelectric focusing, Kleihauer–Betke test, and flow cytometry should be listed.

Abstract

The identification of the new form of hemoglobin in fetus red blood cells (HbF), different from adult hemoglobin (HbA), was made over one hundred years ago. Since this time, various methods of fetal hemoglobin measurement have been designed. Most of them are based on the different biochemical characteristics of HbF. Fetal hemoglobin's affinity for oxygen is substantially greater than that of adult hemoglobin. The first techniques for the determination of fetal hemoglobin were based on its resistance to denaturation by alkaline solutions. Currently, the measurement of hemoglobin F is an important part of diagnosis of sickle cell disease, thalassemia, hereditary persistence of fetal hemoglobin, and fetomaternal hemorrhage. Amongst the most commonly used and clinically important methods, high-performance liquid chromatography, capillary electrophoresis, isoelectric focusing, capillary isoelectric focusing, Kleihauer–Betke test, and flow cytometry should be listed.

Get Citation

Keywords

Fetal hemoglobin; High-performance liquid chromatography HPLC; Capillary electrophoresis; Isoelectric focusing; Capillary isoelectric focusing; Kleihauer–Betke test

About this article
Title

Methods for determination of fetal hemoglobin

Journal

Acta Haematologica Polonica

Issue

Vol 48, No 2 (2017)

Pages

112-118

Published online

2017-04-01

DOI

10.1016/j.achaem.2016.12.001

Bibliographic record

Acta Haematol Pol 2017;48(2):112-118.

Keywords

Fetal hemoglobin
High-performance liquid chromatography HPLC
Capillary electrophoresis
Isoelectric focusing
Capillary isoelectric focusing
Kleihauer–Betke test

Authors

Elżbieta Górska
Anna Lemańska
Katarzyna Popko

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