open access
Methods for determination of fetal hemoglobin
Affiliations- Zakład Diagnostyki Laboratoryjnej i Immunologii Klinicznej Wieku Rozwojowego, Warszawski Uniwersytet Medyczny, Warszawa, Polska
- Studenckie Koło Naukowe przy Zakładzie Diagnostyki Laboratoryjnej i Immunologii Klinicznej Wieku Rozwojowego, Warszawski Uniwersytet Medyczny, Kierownik: Prof dr hab. n. med. Urszula Demkow, Warszawa, Polska
open access
Abstract
The identification of the new form of hemoglobin in fetus red blood cells (HbF), different from adult hemoglobin (HbA), was made over one hundred years ago. Since this time, various methods of fetal hemoglobin measurement have been designed. Most of them are based on the different biochemical characteristics of HbF. Fetal hemoglobin's affinity for oxygen is substantially greater than that of adult hemoglobin. The first techniques for the determination of fetal hemoglobin were based on its resistance to denaturation by alkaline solutions. Currently, the measurement of hemoglobin F is an important part of diagnosis of sickle cell disease, thalassemia, hereditary persistence of fetal hemoglobin, and fetomaternal hemorrhage. Amongst the most commonly used and clinically important methods, high-performance liquid chromatography, capillary electrophoresis, isoelectric focusing, capillary isoelectric focusing, Kleihauer–Betke test, and flow cytometry should be listed.
Abstract
The identification of the new form of hemoglobin in fetus red blood cells (HbF), different from adult hemoglobin (HbA), was made over one hundred years ago. Since this time, various methods of fetal hemoglobin measurement have been designed. Most of them are based on the different biochemical characteristics of HbF. Fetal hemoglobin's affinity for oxygen is substantially greater than that of adult hemoglobin. The first techniques for the determination of fetal hemoglobin were based on its resistance to denaturation by alkaline solutions. Currently, the measurement of hemoglobin F is an important part of diagnosis of sickle cell disease, thalassemia, hereditary persistence of fetal hemoglobin, and fetomaternal hemorrhage. Amongst the most commonly used and clinically important methods, high-performance liquid chromatography, capillary electrophoresis, isoelectric focusing, capillary isoelectric focusing, Kleihauer–Betke test, and flow cytometry should be listed.
Keywords
Fetal hemoglobin; High-performance liquid chromatography HPLC; Capillary electrophoresis; Isoelectric focusing; Capillary isoelectric focusing; Kleihauer–Betke test
About this articleTitle
Methods for determination of fetal hemoglobin
Journal
Issue
Pages
112-118
Published online
2017-04-01
DOI
10.1016/j.achaem.2016.12.001
Bibliographic record
Acta Haematol Pol 2017;48(2):112-118.
Keywords
Fetal hemoglobin
High-performance liquid chromatography HPLC
Capillary electrophoresis
Isoelectric focusing
Capillary isoelectric focusing
Kleihauer–Betke test
Authors
Elżbieta Górska
Anna Lemańska
Katarzyna Popko