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Gene expression analysis in the pathogenesis of chronic immune thrombocytopenia in children
Affiliations- Klinika Pediatrii, Hematologii i Onkologii Collegium Medicum im. Ludwika Rydygiera, Uniwersytet Mikołaja Kopernika w Toruniu, Bydgoszcz, Polska
- Department of Pathology, School of Medicine, Stanford University, Stanford, CA, USA
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Abstract
Background
A complex multifactorial dysregulation of immune system, including cytokines and autoantibodies production, and also proteins modification underlay the primary mechanism of immune thrombocytopenia (ITP). Specific genetic profile of ITP patients is presumed, especially those with a long and complex natural history of the disease.
Aim of the study
Gene expression analysis of VNN1 and PPARγ in children with immune thrombocytopenia.
Materials and methods
Candidate genes were identified with microarray cDNA procedure. For the validation of VNN1 and PPARγ expression changes we used qRT-PCR performed comparatively in samples of newly diagnosed ITP (ndITP, n=16), chronic ITP (cITP, n=8) and patients without thrombocytopenia (n=5).
Results
We analyzed the data of patients, followed for at least 12 months after ITP diagnosis. No significant differences of VNN1 expression profile were found in between groups (p>0.05). Lower signatures of mean PPARγ normalized expression values were noticed in chronic ITP patients in contrast to newly diagnosed ITP subjects (p=0.009). Differences between VNN1/PPARγ ratio values found in ndITP group comparing to subjects with progression to cITP were close to statistical significance (p=0.054).
Conclusions
Analysis of the VNN1 and PPARγ expression profiles utility in children diagnosed with ITP requires further investigation.
Abstract
Background
A complex multifactorial dysregulation of immune system, including cytokines and autoantibodies production, and also proteins modification underlay the primary mechanism of immune thrombocytopenia (ITP). Specific genetic profile of ITP patients is presumed, especially those with a long and complex natural history of the disease.
Aim of the study
Gene expression analysis of VNN1 and PPARγ in children with immune thrombocytopenia.
Materials and methods
Candidate genes were identified with microarray cDNA procedure. For the validation of VNN1 and PPARγ expression changes we used qRT-PCR performed comparatively in samples of newly diagnosed ITP (ndITP, n=16), chronic ITP (cITP, n=8) and patients without thrombocytopenia (n=5).
Results
We analyzed the data of patients, followed for at least 12 months after ITP diagnosis. No significant differences of VNN1 expression profile were found in between groups (p>0.05). Lower signatures of mean PPARγ normalized expression values were noticed in chronic ITP patients in contrast to newly diagnosed ITP subjects (p=0.009). Differences between VNN1/PPARγ ratio values found in ndITP group comparing to subjects with progression to cITP were close to statistical significance (p=0.054).
Conclusions
Analysis of the VNN1 and PPARγ expression profiles utility in children diagnosed with ITP requires further investigation.
Keywords
Immune thrombocytopenia; Genes; Expression; Children
About this articleTitle
Gene expression analysis in the pathogenesis of chronic immune thrombocytopenia in children
Journal
Issue
Pages
76-82
Published online
2014-01-01
Page views
90
Article views/downloads
425
DOI
10.1016/j.achaem.2013.11.004
Bibliographic record
Acta Haematol Pol 2014;45(1):76-82.
Keywords
Immune thrombocytopenia
Genes
Expression
Children
Authors
Monika Richert-Przygońska
Bing Zhang
James L. Zehnder
Mariusz Wysocki