Impaired endotoxin tolerance on example of IL6 in B-CLL

Abstract

IL-6 is considered a key cytokine involved in immune organism response, hematopoietic processes and inflammatory reactions, based on multidirectional auto and paracrine auction Its production is usually transient and tightly regulated.

By the means of Real Time PCR we studied IL-6 expression on mRNA level (RQ) in B-CLL lymphocytes and in CD19+ subpopulation of normal B lymphocytes before and after 30 min and 24h LPS stimulation. The IL-6 expression level (RQ) in B-CLL cells was significantly lower (p=0.00348) compared with the subpopulation of CD19+ normal lymphocytes. 30 min LPS stimulation resulted in increase of IL6 mRNA expression, both in normal and B-CLL lymphocytes. IL6 mRNA expression level decreased after 24h LPS stimulation (p=0.012970) in normal lymphocytes, whereas B-CLL cells showed constant increase (p=0.000001). These data seem to indicate an aberrant endotoxin tolerance in B-CLL.