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Emerging spread of β-thalassemia trait in Nigeria


- Haematology Division, Department of Medical Laboratory Science, College of Health Sciences, Ladoke Akintola University of Technology, P.M.B. 4400, Osogbo, Osun State, Nigeria
- Haematology Division, Department of Medical Laboratory Science, College of Medicine & Health Sciences, Afe Babalola University, P.M.B. 5454, Ado – Ekiti, Ekiti State, Nigeria
open access
Abstract
Background
Chronic anaemia mainly thalassemia and sickle cell anaemia are inherited disorders of haemoglobin. Presently about 7% of the world's populations are carriers of a potentially pathological haemoglobin gene. Sickle cell disease is a common haemoglobinopathy in Nigeria but recently cases of β-thalassemia traits are becoming prominent. This study aimed at screening for β-thalassemia in adults and children with chronic anaemia in Nigeria by assessing the patients’ level of haemoglobin F, haemoglobin A2 and red cell indices.
Materials and methods
Haemoglobin F and HbA2 were determined in the chronic anaemia patients by Alkaline Denaturation Method and Beta-Thal HbA2 Quick Column Procedure respectively. Haemoglobin genotype was determined by Haemoglobin Electrophoresis at alkaline medium while Complete Blood count was estimated using Sysmex KX-2IN Autoanalyser.
Results
The mean HbF, HbA2, HCT, MCV, MCH and MCHC of the children and adults are 2.56±0.46 and 2.45±0.87 (%); 2.05±0.25 and 1.89±0.60 (%); 0.21±0.31 and 0.21±0.36 (L/L); 81.58±12.59 and 78.69±14.11 (fL); 22.74±5.39 and 23.07±7.36 (pg); 27.52±3.84 and 31.23±14.32 (g/l) respectively. Four percent (2 subjects) of each adult and children population had increased HbF level (>1.5%) and HbA2 levels (>2.8%) and these subjects are composed of 2 children with haemoglobin genotype AA and two adult with haemoglobin genotypes SS.
Conclusions
The outcome of this study reiterates the emergence of β-thalassemia traits and iron deficiency anaemia in different parts of Nigeria irrespective of their haemoglobin genotype status. This requires adequate specialized intervention for their diagnosis and treatment. There is therefore the need for subsequent molecular analysis to determine the β-thalassemia genes present in the studied community.
Abstract
Background
Chronic anaemia mainly thalassemia and sickle cell anaemia are inherited disorders of haemoglobin. Presently about 7% of the world's populations are carriers of a potentially pathological haemoglobin gene. Sickle cell disease is a common haemoglobinopathy in Nigeria but recently cases of β-thalassemia traits are becoming prominent. This study aimed at screening for β-thalassemia in adults and children with chronic anaemia in Nigeria by assessing the patients’ level of haemoglobin F, haemoglobin A2 and red cell indices.
Materials and methods
Haemoglobin F and HbA2 were determined in the chronic anaemia patients by Alkaline Denaturation Method and Beta-Thal HbA2 Quick Column Procedure respectively. Haemoglobin genotype was determined by Haemoglobin Electrophoresis at alkaline medium while Complete Blood count was estimated using Sysmex KX-2IN Autoanalyser.
Results
The mean HbF, HbA2, HCT, MCV, MCH and MCHC of the children and adults are 2.56±0.46 and 2.45±0.87 (%); 2.05±0.25 and 1.89±0.60 (%); 0.21±0.31 and 0.21±0.36 (L/L); 81.58±12.59 and 78.69±14.11 (fL); 22.74±5.39 and 23.07±7.36 (pg); 27.52±3.84 and 31.23±14.32 (g/l) respectively. Four percent (2 subjects) of each adult and children population had increased HbF level (>1.5%) and HbA2 levels (>2.8%) and these subjects are composed of 2 children with haemoglobin genotype AA and two adult with haemoglobin genotypes SS.
Conclusions
The outcome of this study reiterates the emergence of β-thalassemia traits and iron deficiency anaemia in different parts of Nigeria irrespective of their haemoglobin genotype status. This requires adequate specialized intervention for their diagnosis and treatment. There is therefore the need for subsequent molecular analysis to determine the β-thalassemia genes present in the studied community.
Keywords
Chronic anaemia; β-Thalassemia; Haemoglobin F; Haemoglobin A2; Iron deficiency anaemia


Title
Emerging spread of β-thalassemia trait in Nigeria
Journal
Issue
Pages
35-39
Published online
2017-01-01
Page views
107
Article views/downloads
126
DOI
10.1016/j.achaem.2016.11.003
Bibliographic record
Acta Haematol Pol 2017;48(1):35-39.
Keywords
Chronic anaemia
β-Thalassemia
Haemoglobin F
Haemoglobin A2
Iron deficiency anaemia
Authors
Olufemi E. Akanni
Oluwaseyi E. Bamisaye
Temitope T. Alabi