open access

Vol 47, No 3 (2016)
Prace oryginalne / Original research articles
Submitted: 2016-07-14
Published online: 2016-07-01
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Tie-2 expressing monocytes in chronic lymphocytic leukemia

Agnieszka Bojarska-Junak1, Piotr Grundszok1, Małgorzata Waldowska1, Justyna Woś1, Sylwia Chocholska2, Iwona Hus3, Wioleta Kowalska1, Katarzyna Gęca1, Waldemar Tomczak24, Jacek Roliński1
DOI: 10.1016/j.achaem.2016.08.001
·
Acta Haematol Pol 2016;47(3):211-218.
Affiliations
  1. Katedra i Zakład Immunologii Klinicznej, Uniwersytet Medyczny, Kierownik: prof. dr hab. n. med. Jacek Roliński, Lublin, Polska
  2. Katedra i Klinika Hematoonkologii i Transplantacji Szpiku, Uniwersytet Medyczny, Kierownik: dr hab. n. med. Marek Hus, Lublin, Polska
  3. Samodzielna Pracownia Transplantologii Klinicznej, Uniwersytet Medyczny, Kierownik: prof. dr hab. n. med. Iwona Hus, Lublin, Polska
  4. Katedra Interny z Zakładem Pielęgniarstwa Internistycznego, Uniwersytet Medyczny, Kierownik: prof. dr hab. n. med. Jadwiga Daniluk, Lublin, Polska

open access

Vol 47, No 3 (2016)
Prace oryginalne / Original research articles
Submitted: 2016-07-14
Published online: 2016-07-01

Abstract

Introduction

In peripheral blood, monocytes form a heterogeneous population of cells. One particular subset of circulating monocytes is expressing the angiopoietin receptor Tie-2 (Tie2-expressing monocyte; TEM). TEM are characterized by tumor promoting properties. However, the role of TEM in chronic lymphocytic leukemia (CLL) immunopathogenesis remains undefined.

Material and Methods

Here, we evaluated the monocytes with Tie-2 expression (CD14+Tie-2+) in peripheral blood of CLL patients (n=55) and normal subjects (n=15) by flow cytometry. We investigated possible associations between TEM and poor prognostic factors such as CD38 or ZAP-70 expression, Rai stage and unfavorable cytogenetic abnormalities. Moreover, we investigated the association of TEM percentage with CD14++CD16+ monocytes and Treg percentages.

Results

We found that CLL patients had a higher percentage of CD14+Tie-2+ monocytes compared to normal controls. The percentage of TEM was positively associated with ZAP-70 expression as well as with unfavourable cytogenetic changes: del(17p) and/or del(11q). The frequency of TEM increased with the disease stage. We showed no correlation between the percentage of TEM and CD38 expression. The percentage of TEM at diagnosis was associated with white blood cell count as well as with the percentages of CD19+CD5+ lymphocytes and Tregs. The majority of CD14+Tie-2+ cells belonged to the intermediate monocytes subset (CD14++CD16+) while fewer of them were among the classical (CD14++CD16) or non-classical monocyte (CD14+CD16++) subsets. TEM and CD14++CD16+ monocytes have a proangiogenic activity, suppress T-cell activation and promote Treg expansion. The results suggest that monitoring of TEM number and function may provide useful information in determining disease activity.

Abstract

Introduction

In peripheral blood, monocytes form a heterogeneous population of cells. One particular subset of circulating monocytes is expressing the angiopoietin receptor Tie-2 (Tie2-expressing monocyte; TEM). TEM are characterized by tumor promoting properties. However, the role of TEM in chronic lymphocytic leukemia (CLL) immunopathogenesis remains undefined.

Material and Methods

Here, we evaluated the monocytes with Tie-2 expression (CD14+Tie-2+) in peripheral blood of CLL patients (n=55) and normal subjects (n=15) by flow cytometry. We investigated possible associations between TEM and poor prognostic factors such as CD38 or ZAP-70 expression, Rai stage and unfavorable cytogenetic abnormalities. Moreover, we investigated the association of TEM percentage with CD14++CD16+ monocytes and Treg percentages.

Results

We found that CLL patients had a higher percentage of CD14+Tie-2+ monocytes compared to normal controls. The percentage of TEM was positively associated with ZAP-70 expression as well as with unfavourable cytogenetic changes: del(17p) and/or del(11q). The frequency of TEM increased with the disease stage. We showed no correlation between the percentage of TEM and CD38 expression. The percentage of TEM at diagnosis was associated with white blood cell count as well as with the percentages of CD19+CD5+ lymphocytes and Tregs. The majority of CD14+Tie-2+ cells belonged to the intermediate monocytes subset (CD14++CD16+) while fewer of them were among the classical (CD14++CD16) or non-classical monocyte (CD14+CD16++) subsets. TEM and CD14++CD16+ monocytes have a proangiogenic activity, suppress T-cell activation and promote Treg expansion. The results suggest that monitoring of TEM number and function may provide useful information in determining disease activity.

Get Citation

Keywords

Chronic lymphocytic leukemia; Tie-2; monocytes; TEM; CD38; ZAP-70

About this article
Title

Tie-2 expressing monocytes in chronic lymphocytic leukemia

Journal

Acta Haematologica Polonica

Issue

Vol 47, No 3 (2016)

Pages

211-218

Published online

2016-07-01

Page views

127

Article views/downloads

165

DOI

10.1016/j.achaem.2016.08.001

Bibliographic record

Acta Haematol Pol 2016;47(3):211-218.

Keywords

Chronic lymphocytic leukemia
Tie-2
monocytes
TEM
CD38
ZAP-70

Authors

Agnieszka Bojarska-Junak
Piotr Grundszok
Małgorzata Waldowska
Justyna Woś
Sylwia Chocholska
Iwona Hus
Wioleta Kowalska
Katarzyna Gęca
Waldemar Tomczak
Jacek Roliński

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