Serum concentrations of proangiogenic cytokines (VEGF, bFGF) depending on the histopathological types of Hodgkin lymphoma in children – preliminary report

Abstract

Background

The different histological types of classical Hodgkin Lymphoma (cHL) differ from other percentage share of the Reed-Sternberg cells (R-SC) in the affected lymphoid tissue In the Lymphocyte Depletion cHL (LDcHL) type are present almost only R-SC. In turn, in the Nodular Lymphocyte Predominant Hodgkin lymphoma (NLP-HL) in the structure lymphocytes, histiocytes ora “popcorn” cells are present. Angiogenesis, which is necessary for development neoplasma tissue, is stimulated by proangiogenic cytokines including Vascular-Endothelial Growth Factor (VEGF) and basic Fibroblast Growth Factor (bFGF). In HL these cytokines are produced mainly by the R-SC. The aim of the study was to assess the concentrations of VEGF and bFGF in the serum (sVEGF, sbFGF) in different histopathological types childhood HL.

Procedure

37 children with HL were studied: group A – 34 children with CHL and group B – 3 children with NCHL. In the control group there were 20 children. Using enzyme-linked immunosorbent assays we quantified VEGF and bFGF in the serum of the children with HL.

Results

The median sVEGF in group A was 657.137 pg/ml (43.777–1210.52) and was significantly higher (p<0.05) in comparison with group B (446.182 pg/ml; 234.673–609.68) and group C (44.6 pg/ml; 32.2–734.8). In the LDcHL the sVEGF were the highest (865.220, 1032.665 pg/ml).

Conclusions

Conclusions. sVEGF in children with cHL are significantly higher in relation to the NLP-HL at the moment of diagnosis. There is a tendency to different sVEGF in the histopathological types of CHL.