open access

Vol 51, No 1 (2020)
ORIGINAL RESEARCH ARTICLE
Published online: 2020-03-01
Submitted: 2019-05-06
Get Citation

Microbial contamination risk in hematopoietic stem cell products: retrospective analysis of 1996–2016 data

Jolanta Antoniewicz-Papis, Elżbieta Lachert, Aleksandra Rosiek, Magdalena Łętowska
DOI: 10.2478/ahp-2020-0007
·
Acta Haematol Pol 2020;51(1):29-33.

open access

Vol 51, No 1 (2020)
ORIGINAL RESEARCH ARTICLE
Published online: 2020-03-01
Submitted: 2019-05-06

Abstract

Quality assurance and safety of hematopoietic stem cells (HSC) with special emphasis on bacterial and fungal contamination is the prerequisite for any transplantation procedure. The aim was to determine the incidence rate of such contamination during processing of transplantation material with regard to HSC source: peripheral blood stem cell (PBSC), bone marrow (BM), or cord blood (CB). Analysis involved autologous and allogenic products dedicated for patients and comprised in all 4135 donations, including 112 BM (2.70%), 3787 PBSC (91.60%), and 236 CB (5.70%) processed in cell bank over the period 1996–2016. Aerobic and anaerobic contamination was determined.

Analysis of the 20-year data revealed 42 contaminated products: 25 PBSC (0.66% of tested units) and 17 CB (7.20% of tested units). No microbial contamination of BM products was detected. Overall percentage of contaminated products was 1.01%, mostly with (61.36%). Bacterial contamination rate at cell bank is relatively low and processing in a closed system does not seem as crucial as might be expected. This is particularly true for BM components. Equally important are evaluation of donor’s medical status and condition of the puncture site for collection of source material. Implementation of appropriate sample collection procedures should help minimize the risk of false-positive results due to environmental contamination.

Abstract

Quality assurance and safety of hematopoietic stem cells (HSC) with special emphasis on bacterial and fungal contamination is the prerequisite for any transplantation procedure. The aim was to determine the incidence rate of such contamination during processing of transplantation material with regard to HSC source: peripheral blood stem cell (PBSC), bone marrow (BM), or cord blood (CB). Analysis involved autologous and allogenic products dedicated for patients and comprised in all 4135 donations, including 112 BM (2.70%), 3787 PBSC (91.60%), and 236 CB (5.70%) processed in cell bank over the period 1996–2016. Aerobic and anaerobic contamination was determined.

Analysis of the 20-year data revealed 42 contaminated products: 25 PBSC (0.66% of tested units) and 17 CB (7.20% of tested units). No microbial contamination of BM products was detected. Overall percentage of contaminated products was 1.01%, mostly with (61.36%). Bacterial contamination rate at cell bank is relatively low and processing in a closed system does not seem as crucial as might be expected. This is particularly true for BM components. Equally important are evaluation of donor’s medical status and condition of the puncture site for collection of source material. Implementation of appropriate sample collection procedures should help minimize the risk of false-positive results due to environmental contamination.

Get Citation

Keywords

microbial contamination; microbiological control; hematopoietic stem cell; cell transplantation

About this article
Title

Microbial contamination risk in hematopoietic stem cell products: retrospective analysis of 1996–2016 data

Journal

Acta Haematologica Polonica

Issue

Vol 51, No 1 (2020)

Pages

29-33

Published online

2020-03-01

DOI

10.2478/ahp-2020-0007

Bibliographic record

Acta Haematol Pol 2020;51(1):29-33.

Keywords

microbial contamination
microbiological control
hematopoietic stem cell
cell transplantation

Authors

Jolanta Antoniewicz-Papis
Elżbieta Lachert
Aleksandra Rosiek
Magdalena Łętowska

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By "Via Medica sp. z o.o." sp.k., ul. Świętokrzyska 73, 80–180 Gdańsk, Poland
tel.:+48 58 320 94 94, fax:+48 58 320 94 60, e-mail: journals@viamedica.pl