Vol 10, No 3 (2004)
Research paper
Published online: 2004-07-13
Activity of the kinin complex and increased proliferation of arterial intima in patients with generalized atheriosclerosis operated on abdominal aortic aneurysm
Acta Angiologica 2004;10(3):143-151.
Abstract
Background. Cellular proliferation plays an important role in the formation
of neointima in the setting of coronary artery disease (CAD). Increased expression
and acetylation of the H3 histone may be one of the pivotal factors in this process.
The inflammatory response in acute coronary syndrome is associated with activation
of the kininogen leading to increased production of bradykinin and expression
of its receptors. The goal of our study was to evaluate the expression of mRNA
for bradykinin BR1 and BR2 receptors, kallistatin (inhibitor of kallikrein) and
H3 histone in samples of aortic intima in patients with generalized atherosclerosis
operated on abdominal aortic aneurysms.
Material and methods. Fifty one patients (age 63.0 ± 6.7 years) were enrolled. The total mRNA was isolated from tissue samples removed during surgical repair of aortic aneurysms. Quantitative RT-PCR technique was used to analyze the expression of mRNA for BR1, BR2, kallistatin and H3 histone. The results are expressed as the concentration of mRNA for the tested genes per 1 μg of total mRNA (tmRNA). The expression of β-actin gene was used as the control.
Results. In all tissue samples, the expression of mRNA for BR1 and BR2 receptors was found. Subsequently, based on the relative level of expression, patients were divided into two groups: I - expression of BR2 < BR1 (n = 31) and II - BR2 > BR1 (n = 20). In group I, the mean number of BR1 mRNA copies was 3325 ± 754/ug tmRNA and in group II 2426 ± 267/ug tmRNA (p = NS). The mean number of BR2 copies was 818 ± 131/ug tmRNA in group I and 4021 ± 456/ug tmRNA in group II (p < 0.001). In group II there was also a higher number of mRNA copies for kallistatin (3634 ± 204 vs 1198 ± 968/ug tmRNA; p < 0.0001) and H3 histone (7795 ± 532 vs 3869 ± 366/ug tmRNA; p = 0.0063) in comparison to group I. The expression of gene encoding for β-actin was comparable in both groups. A positive correlation between the ratio of BR1/BR2 mRNA copies and number of mRNA copies for kallistatin (r = 0.6571; p < 0.001) and H3 histone (r = 0.3519; p = 0.0131) was found.
Conclusion. In tissue samples of aortic intima of patients with generalized atherosclerosis and aortic aneurysm the increased expression of bradykinin BR2 receptor is associated with the higher expression of kallistatin and H3 histone genes.
Material and methods. Fifty one patients (age 63.0 ± 6.7 years) were enrolled. The total mRNA was isolated from tissue samples removed during surgical repair of aortic aneurysms. Quantitative RT-PCR technique was used to analyze the expression of mRNA for BR1, BR2, kallistatin and H3 histone. The results are expressed as the concentration of mRNA for the tested genes per 1 μg of total mRNA (tmRNA). The expression of β-actin gene was used as the control.
Results. In all tissue samples, the expression of mRNA for BR1 and BR2 receptors was found. Subsequently, based on the relative level of expression, patients were divided into two groups: I - expression of BR2 < BR1 (n = 31) and II - BR2 > BR1 (n = 20). In group I, the mean number of BR1 mRNA copies was 3325 ± 754/ug tmRNA and in group II 2426 ± 267/ug tmRNA (p = NS). The mean number of BR2 copies was 818 ± 131/ug tmRNA in group I and 4021 ± 456/ug tmRNA in group II (p < 0.001). In group II there was also a higher number of mRNA copies for kallistatin (3634 ± 204 vs 1198 ± 968/ug tmRNA; p < 0.0001) and H3 histone (7795 ± 532 vs 3869 ± 366/ug tmRNA; p = 0.0063) in comparison to group I. The expression of gene encoding for β-actin was comparable in both groups. A positive correlation between the ratio of BR1/BR2 mRNA copies and number of mRNA copies for kallistatin (r = 0.6571; p < 0.001) and H3 histone (r = 0.3519; p = 0.0131) was found.
Conclusion. In tissue samples of aortic intima of patients with generalized atherosclerosis and aortic aneurysm the increased expression of bradykinin BR2 receptor is associated with the higher expression of kallistatin and H3 histone genes.
Keywords: kininsbradykinin receptorsH3 histoneatherosclerosisQRT-PCR reactionaorta aneurysm