Vol 16, No 5 (2020)
Research paper
Published online: 2020-10-29

open access

Page views 539
Article views/downloads 642
Get Citation

Connect on Social Media

Connect on Social Media

Analysis of ROS1 gene rearrangement incidence among NSCLC patients with fluorescent in situ hybridization technique

Kamila Wojas-Krawczyk1, Patryk Jasielski1, Paweł Krawczyk1, Tomasz Jankowski1, Magdalena Wójcik-Superczyńska1, Katarzyna Reszka2, Izabela Chmielewska1, Jarosław Buczkowski1, Tomasz Kucharczyk1, Justyna Szumiło3, Jarosław Kołb-Sielecki4, Youssef Sleiman5, Aleksandra Szczęsna6, Tomasz Ciszewski7, Rodryg Ramlau8, Grażyna Jagiełło9, Piotr Krudys10, Janusz Milanowski1
Oncol Clin Pract 2020;16(5):270-275.


Introduction. The rearrangement of the gene encoding ROS protooncogene (ROS1) is observed in a very small percentage (1–2%) of patients with non-small cell lung cancer (NSCLC). The clinical characteristics of ROS1-positive patients are similar to those observed in the group of patients with ALK gene rearrangement. Detection of ROS1 gene rearrangement is an extremely important predictive factor enabling the use of crizotinib in the 1st line of NSCLC patients with stage IIIB or IV. Due to the addition of crizotinib to the list of reimbursed drugs from January 2019, the analysis of this genetic change should be part of a molecular tests panel performed in patients with locally advanced and advanced NSCLC in the qualification for molecularly targeted treatment. Aim of the study. Analysis of ROS1 gene rearrangement incidence among NSCLC patients in stage IIIB or IV qualified for molecularly targeted therapies. Presentation of methodological difficulties with fluorescent in situ hybridization (FISH) technique which is used to detect ROS1 genetic abnormality. Materials and methods. The analysis of ROS1 gene rearrangement was carried out using fluorescent in situ hybridization technique in tissue samples taken from 573 NSCLC patients of non-squamous cell type during routine pathomorphological diagnostics.

Results. The material obtained from the tumor was fixed in formalin and archived in paraffin. Histological material was obtained from 408 patients, and 165 — cytological (cytoblock). A reliable (diagnostic) result of the ROS1 gene rearrangement was obtained in 439 patients (76.61%). The main difficulties for ROS1 gene analysis were low number of cancer cells, as well as high background fluorescence interference and fragmentation of cell nuclei. ROS1 gene rearrangement was detected in 9 patients with adenocarcinoma (1.57% among all patients), including 5 men and 4 women. In 19 patients, other abnormalities regarding the ROS1 gene were observed, primarily the polysomy of the examined ROS1 gene fragment (3.32%). Polysomy did not coexist with the ROS1 rearrangement. Conclusion. Fluorescent in situ hybridization is a useful tool in detecting ROS1 gene rearrangement. The test can be performed in both histological and cytological material (cytoblock). However, the correct fixation of the material and the appropriate number of tumor cells in the tested samples is extremely important for obtaining a reliable result.

Article available in PDF format

View PDF Download PDF file


  1. Raparia K, Villa C, DeCamp MM, et al. Molecular profiling in non-small cell lung cancer: a step toward personalized medicine. Arch Pathol Lab Med. 2013; 137(4): 481–491.
  2. Rosell R, Bivona TG, Karachaliou N. Genetics and biomarkers in personalisation of lung cancer treatment. Lancet. 2013; 382(9893): 720–731.
  3. Sasaki T, Rodig SJ, Chirieac LR, et al. The biology and treatment of EML4-ALK non-small cell lung cancer. Eur J Cancer. 2010; 46(10): 1773–1780.
  4. Takeuchi K, Soda M, Togashi Y, et al. RET, ROS1 and ALK fusions in lung cancer. Nat Med. 2012; 18(3): 378–381.
  5. Jassem J, Krzakowski M. Nowotwory klatki piersiowej. Praktyczny przewodnik dla lekarzy. Wydanie III. Via Medica, Gdańsk 2018.
  6. Birchmeier C, O'Neill K, Riggs M, et al. Characterization of ROS1 cDNA from a human glioblastoma cell line. Proc Natl Acad Sci U S A. 1990; 87(12): 4799–4803.
  7. Charest A, Lane K, McMahon K, et al. Fusion of FIG to the receptor tyrosine kinase ROS in a glioblastoma with an interstitial del(6)(q21q21). Gen Chrom Cancer. 2003; 37(1): 58–71.
  8. Tsao MS, Hirsch RR, Yatabe Y, et al. IASLC Atlas of ALK and ROS1 testing in lung cancer. Second Edition. 2016.
  9. Shaw AT, Ou SHI, Bang YJ, et al. Crizotinib in ROS1-rearranged non-small-cell lung cancer. N Engl J Med. 2014; 371(21): 1963–1971.
  10. Rikova K, Guo A, Zeng Q, et al. Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer. Cell. 2007; 131(6): 1190–1203.
  11. Bergethon K, Shaw AT, Ou SHI, et al. ROS1 rearrangements define a unique molecular class of lung cancers. J Clin Oncol. 2012; 30(8): 863–870.
  12. Mehta A, Saifi M, Batra U, et al. Incidence of -rearranged non-small-cell lung carcinoma in india and efficacy of crizotinib in lung adenocarcinoma patients. Lung Cancer (Auckl). 2020; 11: 19–25.
  13. Shaw AT, Riely GJ, Bang YJ, et al. Crizotinib in ROS1-rearranged advanced non-small-cell lung cancer (NSCLC): updated results, including overall survival, from PROFILE 1001. Ann Oncol. 2019; 30(7): 1121–1126.
  14. Shen L, Qiang T, Li Z, et al. First-line crizotinib versus platinum-pemetrexed chemotherapy in patients with advanced ROS1-rearranged non-small-cell lung cancer. Cancer Med. 2020 [Epub ahead of print]; 13: 1–9.
  15. Gainor JF, Tseng D, Yoda S, et al. Patterns of metastatic spread and mechanisms of resistance to crizotinib in ROS1-positive non-small-cell lung cancer. JCO Precis Oncol. 2017; 10.
  16. Shaw A, Felip E, Bauer T, et al. Lorlatinib in non-small-cell lung cancer with ALK or ROS1 rearrangement: an international, multicentre, open-label, single-arm first-in-man phase 1 trial. Lancet Oncol. 2017; 18(12): 1590–1599.
  17. Zhu VW, Upadhyay D, Schrock AB, et al. TPD52L1-ROS1, a new ROS1 fusion variant in lung adenosquamous cell carcinoma identified by comprehensive genomic profiling. Lung Cancer. 2016; 97: 48–50.