open access

Vol 1, No 2 (2005)
Case report
Published online: 2005-06-08
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Case report of mediastinal precusor lymphoma with myeloid and lymphoid lineage specific antigens coexpression and translocation t(10;11)(p12;q21)

Grzegorz Rymkiewicz, Anna Pastwińska, Agnieszka Druzd-Sitek, Katarzyna Błachnio, Barbara Pieńkowska-Grela, Jan Walewski
Onkol. Prak. Klin 2005;1(2):120-127.

open access

Vol 1, No 2 (2005)
CASE REPORTS
Published online: 2005-06-08

Abstract

We report a case of 22-year-old female with unusual myeloperoxidase (MPO)-positive and cytoplasmatic (cCD3)- -negative mediastinal precusor lymphoma with myeloid and lymphoid lineage specific antigens coexpression. One week before admission to our institution she was diagnosed with precursor B lymphoblastic lymphoma (B-LBL). Primary diagnosis was based on histopathology (HP) and immunohistochemistry (IH) only, however final diagnosis of terminal deoxynucleotidyl transferase (TdT)-positive lymphoma of myeloid and T-cell lymphoid lineage with translocation t(10;11)(p12;q21) was made by correlation of HP/IH, flow cytometry (FCM), classic cytogenetic analysis and fluorescence in situ hybridization (FISH).Using FISH analysis, we showed, the MLLT 10(AF10) gene rearrangement (locus 10p12) and lack of the CALM (locus 11q14) and MLL (locus 11q23) genes rearrangement, which are considered the most frequent fusions partners of MLLT 10(AF10) gene. Fine needle aspiration biopsy (FNAB) of the mediastinal tumor and pleural fluid was performed by computed tomography (CT) guided procedure in order to obtain cells for a FCM, cytogenetic analysis and FISH.

Abstract

We report a case of 22-year-old female with unusual myeloperoxidase (MPO)-positive and cytoplasmatic (cCD3)- -negative mediastinal precusor lymphoma with myeloid and lymphoid lineage specific antigens coexpression. One week before admission to our institution she was diagnosed with precursor B lymphoblastic lymphoma (B-LBL). Primary diagnosis was based on histopathology (HP) and immunohistochemistry (IH) only, however final diagnosis of terminal deoxynucleotidyl transferase (TdT)-positive lymphoma of myeloid and T-cell lymphoid lineage with translocation t(10;11)(p12;q21) was made by correlation of HP/IH, flow cytometry (FCM), classic cytogenetic analysis and fluorescence in situ hybridization (FISH).Using FISH analysis, we showed, the MLLT 10(AF10) gene rearrangement (locus 10p12) and lack of the CALM (locus 11q14) and MLL (locus 11q23) genes rearrangement, which are considered the most frequent fusions partners of MLLT 10(AF10) gene. Fine needle aspiration biopsy (FNAB) of the mediastinal tumor and pleural fluid was performed by computed tomography (CT) guided procedure in order to obtain cells for a FCM, cytogenetic analysis and FISH.
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Keywords

TdT- and MPO-positive; cCD3-negative precusor lymphoma of mediastinum; FISH; translocation t(10; 11)(p12; q21); MLLT10(AF10) gene rearrangement

About this article
Title

Case report of mediastinal precusor lymphoma with myeloid and lymphoid lineage specific antigens coexpression and translocation t(10;11)(p12;q21)

Journal

Oncology in Clinical Practice

Issue

Vol 1, No 2 (2005)

Article type

Case report

Pages

120-127

Published online

2005-06-08

Bibliographic record

Onkol. Prak. Klin 2005;1(2):120-127.

Keywords

TdT- and MPO-positive
cCD3-negative precusor lymphoma of mediastinum
FISH
translocation t(10
11)(p12
q21)
MLLT10(AF10) gene rearrangement

Authors

Grzegorz Rymkiewicz
Anna Pastwińska
Agnieszka Druzd-Sitek
Katarzyna Błachnio
Barbara Pieńkowska-Grela
Jan Walewski

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