open access

Vol 17, No 5 (2021)
Research paper
Early publication date: 2021-10-25
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Potential role of BRCA1 protein expression as a prognostic tissue biomarker in breast carcinoma: an immunohistochemical and clinicopathologic study from South India

Reeba Mary Issac1, Prema Saldanha2, Jessy Mangalathu Mathai1, Rebecca Mathews1, Bindu Kumari3, Tiju Chacko4
DOI: 10.5603/OCP.2021.0031
·
Oncol Clin Pract 2021;17(5):205-211.
Affiliations
  1. Department of Pathology, Pushpagiri Institute of Medical Sciences and Research Center, Tiruvalla, Kerala, India
  2. Department of Pathology, Yenepoya Medical College, Yenepoya University, Deralakatte Mangaluru, Karnataka, India
  3. Department of Pathology, Sree Uthradom Thirunal Academy of Medical Sciences, Trivandrum Kerala, India
  4. Kribs Bionest, Cochin, Kerala, India

open access

Vol 17, No 5 (2021)
ORIGINAL ARTICLES
Early publication date: 2021-10-25

Abstract

Introduction. BRCA1 dysfunction is a hallmark of both hereditary and sporadic breast cancer. BRCA1 protein expression can be lost by germline mutation, somatic mutation or promoter hypermethylation. This study aimed to explore BRCA1 dysfunction in breast cancer patients by immunohistochemistry and to study its association with prognostic factors.

Material and methods. BRCA1 protein expression was assessed by immunohistochemistry on formalin fixed paraffin embedded tissue blocks of 110 invasive breast carcinoma patients. Furthermore, the clinical findings and tumor features associated with BRCA1 dysfunction were characterized.

Results. Reduced BRCA1 immunoreactivity was observed in 19% of breast cancer cases. Although these patients presented with aggressive tumor characteristics, statistical significance was observed only with presence of lymphovascular emboli (p < 0.05). These results suggest that loss of BRCA1 protein expression is associated with an aggressive phenotype of breast carcinoma.

Conclusions. Immunohistochemistry for BRCA1 protein expression in tumor tissues may provide a less expensive screening tool to identify BRCA1 dysfunction due to genetic or epigenetic alterations.

Abstract

Introduction. BRCA1 dysfunction is a hallmark of both hereditary and sporadic breast cancer. BRCA1 protein expression can be lost by germline mutation, somatic mutation or promoter hypermethylation. This study aimed to explore BRCA1 dysfunction in breast cancer patients by immunohistochemistry and to study its association with prognostic factors.

Material and methods. BRCA1 protein expression was assessed by immunohistochemistry on formalin fixed paraffin embedded tissue blocks of 110 invasive breast carcinoma patients. Furthermore, the clinical findings and tumor features associated with BRCA1 dysfunction were characterized.

Results. Reduced BRCA1 immunoreactivity was observed in 19% of breast cancer cases. Although these patients presented with aggressive tumor characteristics, statistical significance was observed only with presence of lymphovascular emboli (p < 0.05). These results suggest that loss of BRCA1 protein expression is associated with an aggressive phenotype of breast carcinoma.

Conclusions. Immunohistochemistry for BRCA1 protein expression in tumor tissues may provide a less expensive screening tool to identify BRCA1 dysfunction due to genetic or epigenetic alterations.

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Keywords

breast cancer; BRCA1; biomarker; immunohistochemistry; PARP inhibitor

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Title

Potential role of BRCA1 protein expression as a prognostic tissue biomarker in breast carcinoma: an immunohistochemical and clinicopathologic study from South India

Journal

Oncology in Clinical Practice

Issue

Vol 17, No 5 (2021)

Article type

Research paper

Pages

205-211

Early publication date

2021-10-25

DOI

10.5603/OCP.2021.0031

Bibliographic record

Oncol Clin Pract 2021;17(5):205-211.

Keywords

breast cancer
BRCA1
biomarker
immunohistochemistry
PARP inhibitor

Authors

Reeba Mary Issac
Prema Saldanha
Jessy Mangalathu Mathai
Rebecca Mathews
Bindu Kumari
Tiju Chacko

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