open access

Vol 17, No 4 (2021)
Research paper
Published online: 2021-08-16
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Twist expression and content of tumour-associated macrophages in endometrial carcinoma

Irina Nesina1, Natalia Iurchenko1, Sergey Nespriadko2, Lubov Buchynska1
DOI: 10.5603/OCP.2021.0026
·
Oncol Clin Pract 2021;17(4):139-147.
Affiliations
  1. R.E. Kavetsky Institute of Experimental Pathology, Oncology, and Radiobiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
  2. National Cancer Institute, Ministry of Health of Ukraine, Kyiv, Ukraine

open access

Vol 17, No 4 (2021)
ORIGINAL ARTICLES
Published online: 2021-08-16

Abstract

Introduction. This study aimed to relations between the expression of the Twist transcription factor, the content of tumour-associated macrophages (TAMs), and clinicopathological indicators of tumour progression in patients with stages I–II and III endometrial cancer (EC).

Material and methods. Surgical specimen from 45 patients with endometrioid carcinoma of the endometrium (ECE) (average age — 60.1 ± 2.3 y.o.) were investigated using morphological, immunohistochemical, flow cytofluorometry and statistical methods.

Results. Nuclear expression of Twist was determined in 47.1% of ECE samples with individual fluctuations in the range of 6.3–43.0%, which was 16.6 ± 2.9% on average. Twist expression in G3 endometrial tumours and those with deep invasion into the myometrium tended to increase (21.4 ± 4.3 and 18.0 ± 3.5%, respectively) as compared with the expression of this marker in G2-tumors and the ones, invading < 1/2 of the myometrium (13.2 ± 3.3 and 16.7 ± 3.9%, respectively). Positive expression of Twist in ECE was associated with reduced expression of E-cadherin (44.3 ± 3.8%) and increased expression of vimentin (33.9 ± 3.4%), the content of TAMs in the stromal component of the tumour (30.2 ± 3.7 cells/f.v.), and microvessels density (MVD) (46.5 ± 5.4 vessels/ mm2) as compared with the same indices for ECE with negative expression of Twist (61.4 ± 4.7%, p < 0.05; 14.6 ± 3.1%, p < 0.05; 18.0 ± 2.4 cells/f.v., p < 0.05 and 34.3 ± 4.7 vessels/mm2, respectively).

Conclusions. Higher content of stromal TAMs and higher MVD are observed in Twist-positive endometrial carcinomas as compared with the same indices in Twist-negative neoplasms which are associated with different morphological specificities of invasive processes in the endometrium.

Abstract

Introduction. This study aimed to relations between the expression of the Twist transcription factor, the content of tumour-associated macrophages (TAMs), and clinicopathological indicators of tumour progression in patients with stages I–II and III endometrial cancer (EC).

Material and methods. Surgical specimen from 45 patients with endometrioid carcinoma of the endometrium (ECE) (average age — 60.1 ± 2.3 y.o.) were investigated using morphological, immunohistochemical, flow cytofluorometry and statistical methods.

Results. Nuclear expression of Twist was determined in 47.1% of ECE samples with individual fluctuations in the range of 6.3–43.0%, which was 16.6 ± 2.9% on average. Twist expression in G3 endometrial tumours and those with deep invasion into the myometrium tended to increase (21.4 ± 4.3 and 18.0 ± 3.5%, respectively) as compared with the expression of this marker in G2-tumors and the ones, invading < 1/2 of the myometrium (13.2 ± 3.3 and 16.7 ± 3.9%, respectively). Positive expression of Twist in ECE was associated with reduced expression of E-cadherin (44.3 ± 3.8%) and increased expression of vimentin (33.9 ± 3.4%), the content of TAMs in the stromal component of the tumour (30.2 ± 3.7 cells/f.v.), and microvessels density (MVD) (46.5 ± 5.4 vessels/ mm2) as compared with the same indices for ECE with negative expression of Twist (61.4 ± 4.7%, p < 0.05; 14.6 ± 3.1%, p < 0.05; 18.0 ± 2.4 cells/f.v., p < 0.05 and 34.3 ± 4.7 vessels/mm2, respectively).

Conclusions. Higher content of stromal TAMs and higher MVD are observed in Twist-positive endometrial carcinomas as compared with the same indices in Twist-negative neoplasms which are associated with different morphological specificities of invasive processes in the endometrium.

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Keywords

endometrioid carcinoma of endometrium; Twist; tumour-associated macrophages (CD163); microvessels density (CD31)

About this article
Title

Twist expression and content of tumour-associated macrophages in endometrial carcinoma

Journal

Oncology in Clinical Practice

Issue

Vol 17, No 4 (2021)

Article type

Research paper

Pages

139-147

Published online

2021-08-16

DOI

10.5603/OCP.2021.0026

Bibliographic record

Oncol Clin Pract 2021;17(4):139-147.

Keywords

endometrioid carcinoma of endometrium
Twist
tumour-associated macrophages (CD163)
microvessels density (CD31)

Authors

Irina Nesina
Natalia Iurchenko
Sergey Nespriadko
Lubov Buchynska

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