99mTc-EDDA/HYNIC-TOC in management of patients with head and neck somatostatin receptor positive tumors
Department of Nuclear Medicine, University Hospital Center Zagreb, School of Medicine, University of Zagreb, Zagreb, Croatia
[Received 27 V 2016; Accepted 4 VII 2016]
Abstract
BACKGROUND: Aim of this study was to determine the value of technetium-99m-hydrazinonicotinyl-Tyr3-octreotide (99mTc-EDDA/HYNIC-TOC) in patients with somatostatin receptor (SSR) positive tumors of head and neck region.
MATERIAL AND METHODS: A total number of 16 patients were enrolled in this study. Planar whole body (WB) and single photon emission computed tomography (SPECT) images were acquired at 2 and 4 hours after the injection of approximately 670 MBq of 99mTc-EDDA/HYNIC-TOC. Additional single photon emission computed tomography/computed tomography (SPECT/CT) images of the head and neck region were acquired at 4h post tracer injection. Clinical and imaging follow up were taken as the reference standard.
RESULTS: There were 10 female and 6 male patients of age 57.7 ± 12.9 years (58.5; 32-78) years. 99mTc-EDDA/HYNIC-TOC somatostatin receptor scintigraphy (SRS) was TP in 13 patients, TN in two and FP in one. Follow up period for SRS was 31.1 ± 19.4 (29; 2-63) months. 99mTc-EDDA/HYNIC-TOC scintigraphy provided additional information in 50% of patients, with impact on patient management in the same percentage of patients. Distant metastases were found in nine out of 16 patients (56%). 99mTc-EDDA/HYNIC-TOC SRS had sensitivity of 100% (75.3-100%), specificity of 66.7% (9.4-99.2%), accuracy of 93.7%, positive predictive value of 92.9% (66.1-99.8%), and negative predictive value of 100% (15.8-100%).
CONCLUSION: Somatostatin receptor scintigraphy using 99mTc-EDDA/HYNIC-TOC is very useful imaging method in the evaluation of patients with SSR positive tumors of head and neck region.
KEY words: neuroendocrine tumors, receptors, somatostatin, tomography, emission-computed, single-photon, technetium 99m EDDA-HYNIC-Tyr (3)-octreotide
Nucl Med Rev 2016; 19, 2: 74-80
Background
Somatostatin receptor scintigraphy (SRS) is considered as an imaging modality of choice for many neuroendocrine tumors (NETs). NETs of head and neck are rare group of neoplasms. NETs originate from neural crest cells and have both neural and endocrine cell features [1]. Head and neck NETs can be divided in two groups: epithelial derived tumors (typical carcinoid, atypical carcinoid and small cell carcinoma) and neurally derived tumors (paraganglioma, olfactory neuroblastoma) [2]. Rarely, NETs from other sites can also metastasize to the head and neck region [3]. Some other types of head and neck tumors; medullary thyroid carcinoma (MTC), meningioma, Hurthle cell carcinoma, also showed somatostatin receptors (SSRs) on the cell membrane surface [4].
SRS is indicated in the staging of SSR overexpressing tumors, restaging, detection of eligibility for “cold” somatostatin analogues (SSAs) or somatostatin receptors directed radionuclide therapy (peptide radionuclide receptor therapy – PRRT). SRS is useful in monitoring response to “cold” SSAs and in detection of primary SSR positive tumors [5, 6].
The radiopharmaceutical 111Indium-octreotide (OctreoScan™, Mallinckrodt, Petten, Netherlands) has been considered as the gold standard for the management of NET patients [7-9]. Technetium-99m (99mTc) labeled hydrazinonicotinyl-Tyr3-octreotide (EDDA/HYNIC-TOC, Tektrotyd) has advantages of short half-life, lower radiation burden; so higher dosage can be administered producing better image quality. Physical characteristics of 99mTc are more suited for gamma cameras and single photon emission computed tomography (SPECT) imaging [10-12]. 99mTc labeled EDDA/HYNIC-TOC is also a good alternative to Gallium-68 radiotracers (labeled peptides: DOTA-TOC, DOTA-NOC, DOTA-TATE) where PET/CT or Gallium-68 generators are not available.
MTC is a rare malignancy and in comparison with other types of thyroid carcinoma, MTC is more demanding and more difficult to treat with higher rates of recurrence and mortality. Different radiopharmaceuticals are used for detection of occult metastatic disease and SRS with 99mTc-EDDA/HYNIC-TOC is the promising method [13-15].
The aim of this study was to determine the value of 99mTc EDDA/HYNIC-TOC in patients with neuroendocrine and other SSR positive tumors of head and neck region in terms of sensitivity, specificity, diagnostic accuracy. Impact on patient management was also evaluated.
Material and methods
Patients, radiopharmaceutical and imaging
All patients gave their written informed consent for the study. This is a retrospective study of 16 patients who referred to our Department for a routine examination between February 2011 and March 2016. 99mTc-EDDA/HYNIC-TOC was prepared from a commercially available kit (Tektrotyd, Polatom, Otwock, Poland) following the manufacturer’s instructions. Patients were intravenously injected with an average activity of 670 MBq of the tracer. Imaging was performed at 2 and 4 hours with a double-headed gamma camera (SymbiaT; Siemens Medical Solutions, Erlangen, Germany). Planar whole body (WB) and single photon emission computed tomography (SPECT) images were acquired at 2 and 4 h. Single photon emission computed tomography/computed tomography (SPECT/CT) images of the head and neck region, or other region of interest, were acquired at 4 h. SPECT image data were reconstructed using FLASH-3D iterative reconstruction with 8 iterations and 8 subsets [16]. All images were reconstructed using syngoMI workstation (SynogMI VA60B; Siemens Medical Solution, Erlangen, Germany).
Any focal tracer accumulation exceeding normal regional tracer uptake was rated as a pathologic tumor uptake. Image analysis was done visually by experienced nuclear medicine physician who performed more than 20099mTc-EDDA/HYNIC-TOC SPECT/CT studies in the past four years.
Quantitative data are presented as their mean ± standard deviation (median; range), if not otherwise stated. Findings on SRS images were classified as true-positive (TP), true-negative (TN), false-positive (FP), or false-negative (FN), as compared to the reference standard (conventional imaging methods, clinical and biochemical follow up).
Results
Patient characteristics are detailed in Table 1. There were 10 female and 6 male patients of age 57.7 ± 12.9 years, (58.5; 32-78) years. Details of the findings of 99mTc-EDDA/HYNIC-TOC images are presented in Table 2. 99mTc-EDDA/HYNIC-TOC images were interpreted as positive in 14 patients and as negative in two patients. Follow up period for SRS was 31.1 ± 19.4 (29; 2-63) months. 99mTc-EDDA/HYNIC-TOC SPECT/CT was TP in 13 patients, TN in two and FP in one. Representative cases are shown in Figures 1-6.
Table 1. Patient and tumor characteristics
| Patient Number | Sex | Age (years) | Follow up (months) | Primary tumor site | Pathohistology | WHO Classification |
|---|---|---|---|---|---|---|
| 1 | M | 52 | 29 | Pharynx | NEC | G3 |
| 2 | F | 43 | 2 | CUP | NEC | G3 |
| 3 | F | 78 | 38 | Lacrimal gland | NET | G2 |
| 4 | F | 65 | 28 | Thyroid | MTC | n/a |
| 5 | M | 61 | 4 | Pharynx | NEC | G3 |
| 6 | F | 42 | 12 | Lacrimal gland | NET | G2 |
| 7 | M | 69 | 63 | Larynx | NEC | G3 |
| 8 | F | 32 | 41 | Meninges | Meningioma | G3 |
| 9 | F | 73 | 28 | Thyroid | Hurthle cell | n/a |
| 10 | F | 67 | 6 | Thyroid | MTC | n/a |
| 11 | M | 71 | 15 | Thyroid | MTC | n/a |
| 12 | F | 53 | 45 | Thyroid | MTC | n/a |
| 13 | F | 52 | 45 | Thyroid | MTC | n/a |
| 14 | F | 56 | 59 | Thyroid | MTC | n/a |
| 15 | M | 45 | 53 | Thyroid | MTC | n/a |
| 16 | M | 64 | 29 | CUP | NEC | G3 |
M – male; F – female; CUP – cancer of unknown primary; NEC – neuroendocrine cancer; NET – neuroendocrine tumor; MTC – medullary thyroid cancer; G2 – grade 2; G3 – grade 3; n/a – not applicable
Table 2. Results of visual image analysis
| Patient Number | 99mTc-EDDA/HYNIC-TOC Positive scan |
Lesions locations | Distant metastasis |
|---|---|---|---|
| 1 | Yes | Nasopharynx, Base of skull, NLN (M) | No |
| 2 | Yes | Brain (M), ALN (M) | Yes |
| 3 | Yes | Orbital cavity, Bone (Sphenoid) | No |
| 4 | Yes | Bone (sacrum) | Yes |
| 5 | Yes | Laryngopharynx, NLN (M) | No |
| 6 | Yes | Orbital cavity | No |
| 7 | Yes | Bone (M) | Yes |
| 8 | Yes | Brain | No |
| 9 | Yes | Lungs (M) | Yes |
| 10 | Yes | Bone (M) | Yes |
| 11 | Yes | Bone (humerus), Lungs (M) | Yes |
| 12 | Yes | Liver | Yes |
| 13 | No | 0 | No |
| 14 | Yes | Bone (M) | Yes |
| 15 | No | 0 | No |
| 16 | Yes | NLN(M) | Yes |
M – multiple lesions; 0 – no lesions; NLN – neck lymph nodes; ALN – abdominal lymph nodes
Figure 1. A 65-year-old female with medullary thyroid carcinoma (case number 4). 99mTc-EDDA/HYNIC-TOC SPECT/CT revealed distant metastasis in sacrum. SPECT/CT, single photon emission computed tomography/computed tomography
Figure 2. A 61-year-old male with NEC of laryngopharynx (case number 5). 99mTc-EDDA/HYNIC-TOC SPECT/CT showed intensive uptake in neck lymph nodes (bilateral) and in primary tumor of laryngopharynx. NEC, neuroendocrine carcinoma; SPECT/CT, single photon emission computed tomography/computed tomography
Figure 3. A 42-year-old female with NET G2 (Ki67 8%) of lacrimal gland (case number 6). 99mTc-EDDA/HYNIC-TOC SPECT/CT was done as preoperative staging. SPECT/CT (axial, sagittal and coronal view) showed increased radiopharmaceutical uptake in the right orbital cavity. NET G2, neuroendocrine tumor grade 2; SPECT/CT, single photon emission computed tomography/computed tomography
Figure 4. A 78-year-old female with NET G2 of lacrimal gland (case number 3). 99mTc-EDDA/HYNIC-TOC SPECT/CT was done as restaging. SPECT/CT images (axial view) revealed intensive uptake in left orbital cavity. SPECT/CT showed as well intensive uptake of the radiopharmaceutical in both parotid glands – Sjogren’s syndrome. NET G2, neuroendocrine tumor grade 2; SPECT/CT, single photon emission computed tomography/computed tomography
Figure 5. A 43-year-old female with neuroendocrine carcinoma of unknown primary site (case number 2). 99mTc-EDDA/HYNIC-TOC SPECT/CT didn’t reveal primary tumor site. SPECT/CT (axial view) showed increased radiopharmaceutical uptake in frontal and parietal lobe. SPECT/CT, single photon emission computed tomography/computed tomography
Figure 6. A 52-year-old male with neuroendocrine carcinoma of nasopharynx (case number 1). 99mTc-EDDA/HYNIC-TOC SPECT/CT was done as preoperative staging. On SPECT/CT images (axial view) is shown increased radiopharmaceutical uptake in the primary tumor with intensive bone destruction at the base of skull. SPECT/CT, single photon emission computed tomography/computed tomography
Cases number 13 and 15, both MTC, were TN with fairly long follow up period of 45 and 53 months, respectively. During this follow up numerous imaging techniques were performed in both patients including whole body PET/CT, MSCT (of the neck, thorax and abdomen region) and once per year US examination of the neck. Once in a year during follow up period patients were admitted to the hospital and on each occasion one of mentioned imaging modality (PET/CT or MSCT) was performed together with US of the neck region.
In five patients (cases 4, 7, 10, 11 and 14) distant spread of disease to skeletal system was found. In these cases 99mTc-EDDA/HYNIC-TOC findings upstaged disease to stage IV (distant metastasis). This changed initial staging done by conventional imaging methods. In all this cases “cold” SSAs were introduced. In two additional patients (cases 2 and 16) “cold” SSAs were also introduced after positive scintigraphy. Treatment of one patient (case 14) was changed to PRRT after positive scintigraphy scan. In overall, on a clinical basis 99mTc-EDDA/HYNIC-TOC scintigraphy provided additional information in 50% of patients, with impact on patient management in the same percentage of patients. Distant metastases were found in 56% of patients (9/16). Metastatic bone disease was the most common site of distant metastases in 31% of patients (5/16). Other sites were lymph nodes in 25% (4/16), lungs in 12% (2/16), brain in 6% (1/16) and liver in also 6% (1/16). In one patient (case 3) direct extension of tumor from eyelid and orbital cavity to sphenoid bone was seen. In this patient FP result (in terms of NET spread) was seen in both parotid glands due to chronic inflammatory changes of salivary glands (Sjogren’s syndrome, Figure 4).
In group of patients with NEC (patients 1, 2, 5, 7 and 16) PET/CT and US were used in all patients as reference imaging modality for follow up, while in patients 1, 2 and 5 (because of the neck or head and neck involvement) additional MRI of the head and/or neck was performed. No difference between imaging modalities (99mTc EDDA/HYNIC-TOC vs. 18F-FDG PET/CT or MRI) was noted. In group of NEC distant metastasis were found in 60% of the patients (3/5).
The comparison between 99mTc-EDDA/HYNIC-TOC SRS and reference standard (conventional imaging methods, clinical and biochemical follow up) showed that SRS had sensitivity of 100% (75.3%-100%), specificity of 66.7% (9.4%-99.2%), accuracy of 93.7%, positive predictive value of 92.9% (66.1%-99.8%), and negative predictive value of 100% (15.8%–100%).
Discussion
Precise diagnosis and staging are of incremental value in directing therapy for tumors of head and neck region. Surgical resection is still first choice treatment for a patients with head and neck malignancies, including NET, MTC and other types of SSR positive tumors. In a metastatic disease multiple options are available. In patients with positive finding on SRS one of these options are “cold” SSAs. SSAs are used to control hormone related symptoms. Patients using SSA showed symptomatic and biochemical improvement. SSA anti-tumor effects are still under investigation, they have direct impact on proliferative signaling pathways, on activation of apoptosis, on angiogenesis and on tumor stabilization [17, 18].
In a subgroup of MTC, 99mTc-EDDA/HYNIC-TOC SRS showed incremental value in identifying additional metastatic lesions, especially distant metastatic lesions in skeletal system, providing more accurate staging in these patients. In our group 31 % (5/16) of patients were upstaged by SRS, four of them were MTC. Czepczynski et al. showed clinical usefulness of 99mTc-EDDA/HYNIC-TOC in follow up of patients with medullary thyroid carcinoma. 99mTc-EDDA/HYNIC-TOC had sensitivity of 79.5%, specificity of 83.3% and diagnostic accuracy of 80% [15].
Although Hurthle cell carcinomas do not belong to the traditional group of NETs, positive SSR in these tumors could provide, especially in negative radioiodine cases, new treatment option like PRRT [4, 19, 20].
Somatostatin scintigraphy of receptors type 2 with radiolabeled octreotide has been shown to be very useful in the meningioma diagnosis [21-23], in their published study Wang et al. showed that 99mTc-HYNlC-octreotide SPECT/CT SRS is a sensitive method for detecting meningioma [24]. Several authors investigated this topic using 68Ga-DOTA-peptides PET/CT [25]. We had only one patient with meningioma and 99mTc-EDDA/HYNIC-TOC SPECT/CT in this case provided additional information concerning tumor recurrence and extension. Planned radiotherapy target volume was slightly modified based on SPECT/CT data. Improved treatment planning for meningioma using 68Ga-DOTATOC PET/CT is more and more available method [26-28]. Usefulness of 99mTc-EDDA/HYNIC-TOC SPECT/CT in this indication is still to be investigated.
“Cold” SSAs were introduced in cases number 2, 4, 7, 10, 11, 14 and 16. In cases number 2 (CUP NEC) and 16 (CUP NEC) “cold” SSAs were introduced to reduce the number of flushing episodes and to improve general clinical condition of patients. Good clinical response to the treatment with reduced number of flushing episodes was noticed after introduction of long-acting SSA.
In five other cases (four MTC and one NEC of larynx), based on positive expression of somatostatin receptors, “cold” SSAs were introduced as antineoplastic treatment but no beneficial effect and no significant improvement in the natural course of the tumor was noted. More evident was an improvement in clinical symptoms as general weakness and weight loss in all the patients. Currently, randomized control studies referring on antitumor effect in patients with MTC, do not exist and only case series or studies of limited value are published, which were not able to demonstrate any consistent antitumor somatostatin effect [29-32].
According to Clarinet study (Controlled Study of Lanreotide Antiproliferative Response in Neuroendocrine Tumors), usage of long-acting SSA was associated with significantly prolonged progression-free survival among patients with metastatic enteropancreatic neuroendocrine tumors of grade 1 or 2 (Ki-67 < 10%) [33, 34]. This positive antineoplastic result was not seen in our very small group of patients with MTC and NEC (n = 7) that received “cold” SSA.
There are a number of limitations in this study; retrospective design, sample size was small, group consisted of patients with heterogeneous population of different SSR positive primary tumors (NET, MTC, meningioma, Hurthle cell carcinoma and unknown primary NET tumor with metastases in head and neck region in two patients).
In our subgroup of NETs only grade 2 and grade 3 NETs were present, we didn’t have well-differentiated (low grade) NETs and this could have significantly impact study results. Studies investigating larger and more homogeneous populations are needed.
Conclusion
99m-Tc-EDDA/HYNIC-TOC SRS shows high accuracy in detecting SSR positive tumors. SRS is an excellent imaging modality and reliable tool in the evaluation and treatment planning of patients with SSR positive tumors of head and neck region.
Conflicts of interest
There are no conflicts of interest.
Written informed consent was obtained from all patients.
References
- Modlin IM, Lye KD, Kidd M. A 5-decade analysis of 13,715 carcinoid tumors. Cancer 2003; 97: 934-959.
- Subedi N, Prestwich R, Chowdhury F, Patel C, Scarsbrook A. Neuroendocrine tumours of the head and neck: anatomical, functional and molecular imaging and contemporary management. Cancer imaging 2013; 13: 407-422.
- Salama AR, Jham BC, Papadimitriou JC, Scheper MA. Metastatic neuroendocrine carcinomas to the head and neck: report of 4 cases and review of the literature. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009; 108: 242-247.
- Sollini M, Erba PA, Fraternali A et al. PET and PET/CT with 68gallium-labeled somatostatin analogues in Non GEP-NETs Tumors. Scientific World Journal 2014; 2014: 194123.
- Balon HR, Brown TL, Goldsmith SJ et al. The SNM practice guideline for somatostatin receptor scintigraphy 2.0. J Nucl Med Technol 2011; 39: 317-324.
- Chrapko BE, Nocun A, Golebiewska R et al. 99mTc-EDDA/HYNIC-TOC somatostatin receptor scintigraphy in daily clinical practice. Med Sci Monit 2010; 16: MT35-44.
- Bombardieri E, Ambrosini V, Aktolun C et al. 111In-pentetreotide scintigraphy: procedure guidelines for tumour imaging. Eur J Nucl Med Mol imaging 2010; 37: 1441-1448.
- Krenning EF Kwekkeboom DJ, Bakker WH et al. Somatostatin receptor scintigraphy with [111In-DTPA-D-Phe1]- and [123I-Tyr3]-octreotide: the Rotterdam experience with more than 1000 patients. Eur J Nucl Med 1993; 20: 716-731.
- Olsen JO, Fozderac RV, Hinkle G et al. Somatostatin receptor imaging of neuroendocrine tumors with indium-111 pentetreotide (Octreoscan). Semin Nucl Med 1995; 25: 251-261.
- Decristoforo C, Mather SJ, Cholewinski W, Donnemiller E, Riccabona G, Moncayo R. 99mTc-EDDA/HYNIC-TOC: a new 99mTc-labelled radiopharmaceutical for imaging somatostatin receptor-positive tumours; first clinical results and intra-patient comparison with 111In-labelled octreotide derivatives. Eur J Nucl Med 2000; 27: 1318-1325.
- Gabriel M, Decristoforo C, Donnemiller E et al. An intrapatient comparison of 99mTc-EDDA/HYNIC-TOC with 111In-DTPA-octreotide for diagnosis of somatostatin receptor-expressing tumors. J Nucl Med 2003; 44: 708-716.
- Gabriel M, Muehllechner F, Decristoforo C et al. 99mTc-EDDA/HYNIC-Tyr(3)-octreotide for staging and follow-up of patients with neuroendocrine gastro-entero-pancreatic tumors. Q J Nucl Med Mol imaging 2005; 49: 237-244.
- Bangard M, Behe M, Guhlke S et al. Detection of somatostatin receptor-positive tumours using the new 99mTc-tricine-HYNIC-D-Phe1-Tyr3-octreotide: first results in patients and comparison with 111In-DTPA-D-Phe1-octreotide. Eur J Nucl Med 2000; 27: 628-637.
- Baudin E, Lumbroso J, Schlumberger M et al. Comparison of octreotide scintigraphy and conventional imaging in medullary thyroid carcinoma. J Nucl Med 1996; 37: 912-916.
- Czepczynski R, Farisella MG, Kosowicz J et al. Somatostatin receptor scintigraphy using 99mTc-EDDA/HYNIC-TOC in patients with medullary thyroid carcinoma. Eur J Nucl Med Mol imaging 2007; 34: 1635-1645.
- Sowa-Staszczak A, Lenda-Tracz W, Tomaszuk M, Glowa B, Hubalewska-Dydejczyk A. Optimization of image reconstruction method for SFECT studies performed using [99mTc-EDDA/HYNIC] octreotate in patients with neuroendocrine tumors. Nucl Med Rev Cent East Eur 2013; 16: 9-16.
- Appetecchia M, Baldelli R. Somatostatin analogues in the treatment of gastroenteropancreatic neuroendocrine tumours, current aspects and new perspectives. J Exp Clin Cancer Res 2010; 29: 19.
- Baldelli R, Barnabei A, Rizza L et al. Somatostatin analogs therapy in gastroenteropancreatic neuroendocrine tumors: current aspects and new perspectives. Front Endocrinol (Lausanne) 2014; 5: 7.
- Middendorp M, Selkinski I, Happel C, Kranert WT Grunwald F. Comparison of positron emission tomography with [(18)F]FDG and [(68)Ga]DOTATOC in recurrent differentiated thyroid cancer: preliminary data. Q J Nucl Med Mol imaging 2010; 54: 76-83.
- Versari A, Sollini M, Frasoldati A et al. Differentiated thyroid cancer: a new perspective with radiolabeled somatostatin analogues for imaging and treatment of patients. Thyroid 2014; 24: 715-726.
- Arena S, Barbieri F, Thellung S, Pirani P, Corsaro A, Villa V et al. Expression of somatostatin receptor mRNA in human meningiomas and their implication in in vitro antiproliferative activity. J Neurooncol 2004; 66: 155-166.
- Schmidt M, Scheidhauer K, Luyken C et al. Somatostatin receptor imaging in intracranial tumours. Eur J Nucl Med 1998; 25: 675-686.
- Volante M, Brizzi MP Faggiano A et al. Somatostatin receptor type 2A immunohistochemistry in neuroendocrine tumors: a proposal of scoring system correlated with somatostatin receptor scintigraphy. Mod Pathol 2007; 20: 1172-1182.
- Wang S, Yang W, Deng J, Zhang J, Ma F, Wang J. Correlation between 99mTc-HYNIC-octreotide SPECT/CT somatostatin receptor scintigraphy and pathological grading of meningioma. J Neurooncol 2013; 113: 519-526.
- Afshar-Oromieh A, Giesel FL, Linhart HG et al. Detection of cranial meningiomas: comparison of (6)(8)Ga-DOTATOC PET/CT and contrast-enhanced MRI. Eur J Nucl Med Mol imaging 2012; 39: 1409-1415.
- Gehler B, Paulsen F, Oksuz MO et al. [68Ga]-DOTATOC-PET/CT for meningioma IMRT treatment planning. Radiat Oncol 2009; 4: 56.
- Milker-Zabel S, Zabel-du Bois A, Henze M et al. Improved target volume definition for fractionated stereotactic radiotherapy in patients with intracranial meningiomas by correlation of CT MRI, and [68Ga]-DOTATOC-PET Int J Radiat Oncol Biol Phys 2006; 65: 222-227.
- Nyuyki F, Plotkin M, Graf R et al. Potential impact of (68)Ga-DOTATOC PET/CT on stereotactic radiotherapy planning of meningiomas. Eur J Nucl Med Mol imaging 2010; 37: 310-318.
- Modigliani E, Guliana JM, Maroni M et al. Effects of subcutaneous administration of sandostatine (SMS 201.995) in 18 cases of thyroid medullary cancer. Ann Endocrinol (Paris) 1989; 50: 483-488.
- Mahler C, Verhelst J, de Longueville M, Harris A. Long-term treatment of metastatic medullary thyroid carcinoma with the somatostatin analogue octreotide. Clin Endocrinol (Oxf) 1990; 33: 261-269.
- Vainas I, Koussis C, Pazaitou-Panayiotou K et al. Somatostatin receptor expression in vivo and response to somatostatin analog therapy with or without other antineoplastic treatments in advanced medullary thyroid carcinoma. J Exp Clin Cancer Res 2004; 23: 549-559.
- Frank-Raue K, Ziegler R, Raue F. The use of octreotide in the treatment of medullar/ thyroid carcinoma. Horm Metab Res Suppl 1993; 27: 44-47.
- Caplin ME, Pavel M, Cwikla JB et al. Lanreotide in metastatic enteropancreatic neuroendocrine tumors. N Engl J Med 2014; 371: 224-233.
- Caplin ME, Pavel M, Cwikla JB et al. Anti-tumour effects of lanreotide for pancreatic and intestinal neuroendocrine tumours: the CLARINET open-label extension study. Endocr Relat Cancer 2016; 23: 191-199.
Correspondence to: Mate Trogrlic, MD
Department of Nuclear Medicine, University Hospital Center Zagreb,
Kispaticeva 12, 10000 Zagreb, Croatia
Phone: 00385958562875; fax: 0038512376040
E-mail: mate.trogrlic@gmail.com
