open access

Vol 23, No 2 (2020)
Original articles
Published online: 2020-07-31
Submitted: 2020-03-11
Accepted: 2020-06-18
Get Citation

The levels of oxidative and nitrosative stress in patients who had 99mTc-MIBI myocardial perfusion scintigraphy and 99mTc-DMSA, 99mTc-MAG-3 renal scintigraphy

Ebru Salmanoglu, Ergul Belge Kurutas
DOI: 10.5603/NMR.a2020.0019
·
Nucl. Med. Rev 2020;23(2):83-90.

open access

Vol 23, No 2 (2020)
Original articles
Published online: 2020-07-31
Submitted: 2020-03-11
Accepted: 2020-06-18

Abstract

BACKGROUND: Ionizing radiation is a strong stimulator of reactive oxygen specises (ROS) and reactive nitrogen species (RNS). These reactive species may cause oxidative and nitrosative stress. In this study, we aimed to evaluate possible effects of 99mTechnetium (99mTc)-methoxyisobuthylisonitrite (MIBI), 99mTc-dimercaptosuccinic acid (DMSA), 99mTc-mercaptoacetyltriglycine (MAG-3) on oxidative and nitrosative stress biomarkers in patients who were performed myocardial perfusion scintigraphy (MPS) and renal scintigraphy.

MATERIAL AND METHODS: Patients (n = 29) who were referred to nuclear medicine department were chosen as the patient group. They were divided into three subgroups according to the type of disease and 99mTc labelled agent. The first patient group had MPS (n = 9). The second patient group had 99mTc-DMSA renal scintigraphy (n = 12). The third patient group had 99mTc-MAG-3 renal scintigraphy (n = 8). The blood samples were taken from first, second and third patient groups 1 h, 3 h, 45 min after injection of the agent, respectively. The samples were taken from healthy volunteers (n = 25) as a control group. Alterations in catalase (CAT),superoxide dismutase (SOD), malondialdehyde (MDA) levels as oxidative stress biomarkers and nitric oxide (NO) and 3-Nitrotyrosine (3-NTx) levels as nitrosative stress biomarkers in all blood samples were evaluated.

RESULTS:
Results of MPS and renal scintigraphy performed patients were compared with control group separately. CAT, SOD, MDA and 3-NTx levels were higher in the first group than the control group (p < 0.05). Although NO levels were higher in the first group than the control group, it was not statistically significant (p > 0.05). CAT and SOD levels were lower in second and third groups than the control group (p < 0.0 5). However, MDA, NO, 3-NTx levels were higher in second and third groups than the control group (p < 0.05).

CONCLUSIONS:
These results show that oxidative and nitrosative balance is impaired due to ionization radiation. These reactive species might stimulate an adaptive and protective cellular defense mechanism in irradiated cells soon after exposure to radiation. Thereby, this mechanism protect organism from the effects of low dose ionizing radiation.

Abstract

BACKGROUND: Ionizing radiation is a strong stimulator of reactive oxygen specises (ROS) and reactive nitrogen species (RNS). These reactive species may cause oxidative and nitrosative stress. In this study, we aimed to evaluate possible effects of 99mTechnetium (99mTc)-methoxyisobuthylisonitrite (MIBI), 99mTc-dimercaptosuccinic acid (DMSA), 99mTc-mercaptoacetyltriglycine (MAG-3) on oxidative and nitrosative stress biomarkers in patients who were performed myocardial perfusion scintigraphy (MPS) and renal scintigraphy.

MATERIAL AND METHODS: Patients (n = 29) who were referred to nuclear medicine department were chosen as the patient group. They were divided into three subgroups according to the type of disease and 99mTc labelled agent. The first patient group had MPS (n = 9). The second patient group had 99mTc-DMSA renal scintigraphy (n = 12). The third patient group had 99mTc-MAG-3 renal scintigraphy (n = 8). The blood samples were taken from first, second and third patient groups 1 h, 3 h, 45 min after injection of the agent, respectively. The samples were taken from healthy volunteers (n = 25) as a control group. Alterations in catalase (CAT),superoxide dismutase (SOD), malondialdehyde (MDA) levels as oxidative stress biomarkers and nitric oxide (NO) and 3-Nitrotyrosine (3-NTx) levels as nitrosative stress biomarkers in all blood samples were evaluated.

RESULTS:
Results of MPS and renal scintigraphy performed patients were compared with control group separately. CAT, SOD, MDA and 3-NTx levels were higher in the first group than the control group (p < 0.05). Although NO levels were higher in the first group than the control group, it was not statistically significant (p > 0.05). CAT and SOD levels were lower in second and third groups than the control group (p < 0.0 5). However, MDA, NO, 3-NTx levels were higher in second and third groups than the control group (p < 0.05).

CONCLUSIONS:
These results show that oxidative and nitrosative balance is impaired due to ionization radiation. These reactive species might stimulate an adaptive and protective cellular defense mechanism in irradiated cells soon after exposure to radiation. Thereby, this mechanism protect organism from the effects of low dose ionizing radiation.

Get Citation

Keywords

ionizing radiation; oxidative stress; nitrosative stress

About this article
Title

The levels of oxidative and nitrosative stress in patients who had 99mTc-MIBI myocardial perfusion scintigraphy and 99mTc-DMSA, 99mTc-MAG-3 renal scintigraphy

Journal

Nuclear Medicine Review

Issue

Vol 23, No 2 (2020)

Pages

83-90

Published online

2020-07-31

DOI

10.5603/NMR.a2020.0019

Bibliographic record

Nucl. Med. Rev 2020;23(2):83-90.

Keywords

ionizing radiation
oxidative stress
nitrosative stress

Authors

Ebru Salmanoglu
Ergul Belge Kurutas

References (30)
  1. Papagiannopoulou D. Technetium-99m radiochemistry for pharmaceutical applications. J Labelled Comp Radiopharm. 2017; 60(11): 502–520.
  2. Maucksch U, Runge R, Wunderlich G, et al. Comparison of the radiotoxicity of the Tc-labeled compounds Tc-pertechnetate, Tc-HMPAO and Tc-MIBI. Int J Radiat Biol. 2016; 92(11): 698–706.
  3. Blaufox MD, De Palma D, Taylor A, et al. SNMMI Procedure Standard/EANM Practice Guideline for Diuretic Renal Scintigraphy in Adults With Suspected Upper Urinary Tract Obstruction 1.0. Semin Nucl Med. 2018; 48(4): 377–390.
  4. Santos-Cuevas CL, Ferro-Flores G, Rojas-Calderón EL, et al. 99mTc-N2S2-Tat (49-57)-bombesin internalized in nuclei of prostate and breast cancer cells: kinetics, dosimetry and effect on cellular proliferation. Nucl Med Commun. 2011; 32(4): 303–313.
  5. Piron B, Paillas S, Boudousq V, et al. DNA damage-centered signaling pathways are effectively activated during low dose-rate Auger radioimmunotherapy. Nucl Med Biol. 2014; 41: e75–e83.
  6. Spitz DR, Azzam EI, Li JJ, et al. Metabolic oxidation/reduction reactions and cellular responses to ionizing radiation: a unifying concept in stress response biology. Cancer Metastasis Rev. 2004; 23(3-4): 311–322.
  7. Azzam EI, Jay-Gerin JP, Pain D. Ionizing radiation-induced metabolic oxidative stress and prolonged cell injury. Cancer Lett. 2012; 327(1-2): 48–60.
  8. Dröge W. Free radicals in the physiological control of cell function. Physiol Rev. 2002; 82(1): 47–95.
  9. Sies H. Oxidative stress: oxidants and antioxidants. Exp Physiol. 1997; 82(2): 291–295.
  10. Klandorf H, Dyke KV. Oxidative and Nitrosative Stresses: Their Role in Health and Disease in Man and Birds. Oxidative Stress-Molecular Mechanisms and Biological Effects. Volodymyr Lushchak (ed.), 2012. InTech. http://www.intechopen.com/books/oxidative-stress-molecular-mechanisms-and-biological-effects/oxidativeand-nitrosative-stresses-their-role-in-health-and-disease-in-man-and-birds.
  11. Beutler E. Red Cell Metabolism. A Manual of Biochemical Methods. 2nd ed. Grune and Stratton Inc, New York 1984: 68–70.
  12. Fridovich I. Superoxide dismutase. Adv Enzymol. 1974; 41: 35–97.
  13. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95(2): 351–358.
  14. Backus M, Piwnica-Worms D, Hockett D, et al. Microprobe analysis of Tc-MIBI in heart cells: calculation of mitochondrial membrane potential. Am J Physiol. 1993; 265(1 Pt 1): C178–C187.
  15. Arbab AS, Koizumi K, Toyama K, et al. Technetium-99m-tetrofosmin, technetium-99m-MIBI and thallium-201 uptake in rat myocardial cells. J Nucl Med. 1998; 39(2): 266–271.
  16. Kotzerke J, Punzet R, Runge R, et al. 99mTc-labeled HYNIC-DAPI causes plasmid DNA damage with high efficiency. PLoS One. 2014; 9(8): e104653.
  17. Salmanoglu E, Kurutas EB. Induction of oxidative/nitrosative stress following Tc-99m pertechnetate thyroid scintigraphy in human. Cumhuriyet Medical Journal. 2019.
  18. Salmanoglu E, Kurutas EB. Effects of 99mTc-MDP bone scintigraphy on oxidative/nitrosative stress biomarkers in patients. Adv Lab Med Int. 2017; 7: 12–22.
  19. El-Gebaly RH, Rageh MM, Maamoun IK. Radio-protective potential of lipoic acid free and nano-capsule against 99mTc-MIBI induced injury in cardio vascular tissue. J Xray Sci Technol. 2019; 27(1): 83–96.
  20. Cicek E, Yildiz M, Delibas N, et al. Effects of Dynamic Renal Scintigraphy and Bone Scintigraphy Studies on Oxidative Damage in Patients. Spectroscopy Letters. 2009; 42(2): 63–66.
  21. Cesur G, Doguc DK, Yildiz M, et al. Effects of (99m)Tc sestamibi on antioxidant defense system and lipid peroxidation in the heart of Sprague Dawley rats. Toxicol Ind Health. 2014; 30(2): 154–159.
  22. Cicek E, Yildiz M, Delibas N, et al. The effects of 201Tl myocardial perfusion scintigraphy studies on oxidative damage in patients. West Indian Med J. 2009; 58(1): 50–53.
  23. Gurbuz N, Aydin F, et al. Boz, A, The Effects of 99mTechnetium-methylendiphosphonate and 99mTechnetium-methoxyisobutylisonitrile on Erythrocyte Antioxidant Enzyme Activities. Türk Biyokimya Dergisi [Turkish Journal of Biochemistry–Turk J Biochem. 2010; 35(3): 172–176.
  24. Shahin S, Singh SP, Chaturvedi CM. 2.45 GHz microwave radiation induced oxidative and nitrosative stress mediated testicular apoptosis: Involvement of a p53 dependent bax-caspase-3 mediated pathway. Environ Toxicol. 2018; 33(9): 931–945.
  25. Anand AJ, Dzik WH, Imam A, et al. Radiation-induced red cell damage: role of reactive oxygen species. Transfusion. 1997; 37(2): 160–165.
  26. Ciçek E, Yildiz M, Delibaş N, et al. The effects of thyroid scintigraphy studies on oxidative damage in patients. Acta Physiol Hung. 2006; 93(2-3): 131–136.
  27. Kurutas EB. The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutr J. 2016; 15(1): 71.
  28. Lahera V, Goicoechea M, de Vinuesa SG, et al. Endothelial dysfunction, oxidative stress and inflammation in atherosclerosis: beneficial effects of statins. Curr Med Chem. 2007; 14(2): 243–248.
  29. Tritto I, Ambrosio G. The multi-faceted behavior of nitric oxide in vascular "inflammation": catchy terminology or true phenomenon? Cardiovasc Res. 2004; 63(1): 1–4.
  30. Atzeni F, Sarzi-Puttini P, Sitia S, et al. From endothelial dysfunction to atherosclerosis. Autoimmun Rev. 2010; 9(12): 830–834.

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By "Via Medica sp. z o.o." sp.k., Świętokrzyska 73 street, 80–180 Gdańsk, Poland

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail: viamedica@viamedica.pl