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Preparation and evaluation of [61Cu]-thiophene-2-aldehyde thiosemicarbazone for PET studies
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Abstract
MATERIAL AND METHODS: [61Cu]TATS was prepared using copper-61 acetate and in-house made ligand (TATS) in one step. 61Cu was produced via the natZn(p,x)61Cu nuclear reaction (180 μA, 22 MeV, 3.2 h) followed by a two-step chromatography method (222 GBq of 61Cu2+). [61Cu]TATS preparation was optimized for reaction conditions (buffer concentration and temperature). The tracer was finally administered to normal rats for biodistribution studies.
RESULTS: Total radiolabelling of the tracer took 30 minutes with a radiochemical purity of more than 90% (using HPLC and RTLC) and specific activity of about 250–300 Ci/mmol. The complex was stable in the presence of human serum for an hour. The biodistribution of copper cation and the tracer was checked in wild-type rats for up to 2 hours with significant spleen and lung uptake of the tracer.
CONCLUSIONS: The production of 61Cu via the natZn(p,x)61Cu is an efficient and reproducible method with high specific activity leading to the production and preliminary evaluation of [61,Cu]TATS, a potential PET tracer, was reported.
Abstract
MATERIAL AND METHODS: [61Cu]TATS was prepared using copper-61 acetate and in-house made ligand (TATS) in one step. 61Cu was produced via the natZn(p,x)61Cu nuclear reaction (180 μA, 22 MeV, 3.2 h) followed by a two-step chromatography method (222 GBq of 61Cu2+). [61Cu]TATS preparation was optimized for reaction conditions (buffer concentration and temperature). The tracer was finally administered to normal rats for biodistribution studies.
RESULTS: Total radiolabelling of the tracer took 30 minutes with a radiochemical purity of more than 90% (using HPLC and RTLC) and specific activity of about 250–300 Ci/mmol. The complex was stable in the presence of human serum for an hour. The biodistribution of copper cation and the tracer was checked in wild-type rats for up to 2 hours with significant spleen and lung uptake of the tracer.
CONCLUSIONS: The production of 61Cu via the natZn(p,x)61Cu is an efficient and reproducible method with high specific activity leading to the production and preliminary evaluation of [61,Cu]TATS, a potential PET tracer, was reported.
Keywords
copper-61; radiolabelling; biodistribution; thiophene-2-aldehyde thiosemicarbazone
About this articleTitle
Preparation and evaluation of [61Cu]-thiophene-2-aldehyde thiosemicarbazone for PET studies
Journal
Issue
Pages
41-47
Published online
2008-11-05
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504
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2346
Bibliographic record
Nucl. Med. Rev 2008;11(2):41-47.
Keywords
copper-61
radiolabelling
biodistribution
thiophene-2-aldehyde thiosemicarbazone
Authors
Amir R. Jalilian
Mirsaeed Nikzad
Hassan Zandi
Ali Nemati Kharat
Pejman Rowshanfarzad
Mehdi Akhlaghi
Fatemeh Bolourinovin
