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In vitro and biodistribution examinations of Tc-99m-labelled doxorubicin-loaded nanoparticles
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Abstract
MATERIAL AND METHODS: The three prepared nanoparticles were labelled using technetium (Tc-99m) and were tested for their physicochemical colloidal quality, fluctuations, and radiochemical stability. Biodistribution of different-sized radiolabelled colloids were determined by means of scintigraphic imaging studies in healthy male Wistar rats. Images were taken by gamma camera at several times and organ uptakes were estimated by quantitative ROI analysis.
RESULTS: In vitro measurements showed that more than 95% of doxorubicin proportion was permanently adsorbed to human serum albumin. Radiolabelled doxorubicin-loaded particles had high-degree and durable labelling efficiency and particle size stability. Biodistribution results had a close correlation to earlier described results of radiocolloids in similar particle size ranges. In vivo examinations verified that colloid carriers have insignificant size fluctuations after an intravenous application and they show the proper distribution according to their particle size.
CONCLUSIONS: Our investigations verified that different and stable particle sizes make drug carrier HSA nanoparticles possible to apply different drug targeting in a potential clinical use.
Nuclear Med Rev 2011; 14, 2: 55–62
Abstract
MATERIAL AND METHODS: The three prepared nanoparticles were labelled using technetium (Tc-99m) and were tested for their physicochemical colloidal quality, fluctuations, and radiochemical stability. Biodistribution of different-sized radiolabelled colloids were determined by means of scintigraphic imaging studies in healthy male Wistar rats. Images were taken by gamma camera at several times and organ uptakes were estimated by quantitative ROI analysis.
RESULTS: In vitro measurements showed that more than 95% of doxorubicin proportion was permanently adsorbed to human serum albumin. Radiolabelled doxorubicin-loaded particles had high-degree and durable labelling efficiency and particle size stability. Biodistribution results had a close correlation to earlier described results of radiocolloids in similar particle size ranges. In vivo examinations verified that colloid carriers have insignificant size fluctuations after an intravenous application and they show the proper distribution according to their particle size.
CONCLUSIONS: Our investigations verified that different and stable particle sizes make drug carrier HSA nanoparticles possible to apply different drug targeting in a potential clinical use.
Nuclear Med Rev 2011; 14, 2: 55–62
Keywords
doxorubicin; HSA; Tc-99m; nanoparticle; colloid; labelling; biodistribution


Title
In vitro and biodistribution examinations of Tc-99m-labelled doxorubicin-loaded nanoparticles
Journal
Issue
Article type
Research paper
Pages
55-62
Published online
2012-01-04
Page views
945
Article views/downloads
2661
Bibliographic record
Nucl. Med. Rev 2011;14(2):55-62.
Keywords
doxorubicin
HSA
Tc-99m
nanoparticle
colloid
labelling
biodistribution
Authors
Andras Polyak
Elena Alina Palade
Lajos Balogh
Zita Postenyi
Veronika Haasz
Gergely Janoki
Gyozo A. Janoki