open access

Vol 52, No 1 (2018)
Letters to the Editor
Submitted: 2017-08-18
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O6-methylguanine-DNA-methyltransferase immunostaining intensity in glioblastoma

Daniel Jiménez, José Manuel Matamala, Alessandra Chiti, Carmen Vergara, Claudia Tissera, Romulo Melo, Luis Cartier
DOI: 10.1016/j.pjnns.2017.10.014
·
Neurol Neurochir Pol 2018;52(1):116-119.

open access

Vol 52, No 1 (2018)
Letters to the Editor
Submitted: 2017-08-18

Abstract

Immunohistochemistry (IHC) for O6-methylguanine-DNA-methyltransferase (MGMT) has shown heterogeneous results. Cell staining intensity has not been included as a quantifiable variable in IHC analyses. We performed MGMT IHC in 29 patients diagnosed as glioblastoma classifying cells into three categories based on nuclear staining intensity compared with adjacent endothelium. The median proportions of strong-moderate, weak and no staining cells were 10%, 16% and 71%, respectively. The proportion of positive cases for MGMT expression varies from 38% to 52% depending on the classification of weakly stained cells. This letter challenges previous studies that have not included intensity as a variable for IHC analysis.

Abstract

Immunohistochemistry (IHC) for O6-methylguanine-DNA-methyltransferase (MGMT) has shown heterogeneous results. Cell staining intensity has not been included as a quantifiable variable in IHC analyses. We performed MGMT IHC in 29 patients diagnosed as glioblastoma classifying cells into three categories based on nuclear staining intensity compared with adjacent endothelium. The median proportions of strong-moderate, weak and no staining cells were 10%, 16% and 71%, respectively. The proportion of positive cases for MGMT expression varies from 38% to 52% depending on the classification of weakly stained cells. This letter challenges previous studies that have not included intensity as a variable for IHC analysis.

Get Citation

Keywords

O6-Methylguanine-DNA-methyltransferase, Glioblastoma, Staining intensity, Immunohistochemistry

About this article
Title

O6-methylguanine-DNA-methyltransferase immunostaining intensity in glioblastoma

Journal

Neurologia i Neurochirurgia Polska

Issue

Vol 52, No 1 (2018)

Pages

116-119

DOI

10.1016/j.pjnns.2017.10.014

Bibliographic record

Neurol Neurochir Pol 2018;52(1):116-119.

Keywords

O6-Methylguanine-DNA-methyltransferase
Glioblastoma
Staining intensity
Immunohistochemistry

Authors

Daniel Jiménez
José Manuel Matamala
Alessandra Chiti
Carmen Vergara
Claudia Tissera
Romulo Melo
Luis Cartier

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