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O6-methylguanine-DNA-methyltransferase immunostaining intensity in glioblastoma
Affiliations- Laboratory of Biomedical Sciences, Department of Neurological Sciences, Faculty of Medicine, University of Chile, Santiago, Chile
- Neurology Service, Hospital del Salvador, Santiago, Chile
- Biomedical Neuroscience Institute (BNI), Faculty of Medicine, University of Chile, Santiago, Chile
- Instituto de Neurocirugía Dr. Asenjo, Santiago, Chile
- Department of Neurological Science, Faculty of Medicine, University of Chile, Santiago, Chile
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Abstract
Immunohistochemistry (IHC) for O6-methylguanine-DNA-methyltransferase (MGMT) has shown heterogeneous results. Cell staining intensity has not been included as a quantifiable variable in IHC analyses. We performed MGMT IHC in 29 patients diagnosed as glioblastoma classifying cells into three categories based on nuclear staining intensity compared with adjacent endothelium. The median proportions of strong-moderate, weak and no staining cells were 10%, 16% and 71%, respectively. The proportion of positive cases for MGMT expression varies from 38% to 52% depending on the classification of weakly stained cells. This letter challenges previous studies that have not included intensity as a variable for IHC analysis.
Abstract
Immunohistochemistry (IHC) for O6-methylguanine-DNA-methyltransferase (MGMT) has shown heterogeneous results. Cell staining intensity has not been included as a quantifiable variable in IHC analyses. We performed MGMT IHC in 29 patients diagnosed as glioblastoma classifying cells into three categories based on nuclear staining intensity compared with adjacent endothelium. The median proportions of strong-moderate, weak and no staining cells were 10%, 16% and 71%, respectively. The proportion of positive cases for MGMT expression varies from 38% to 52% depending on the classification of weakly stained cells. This letter challenges previous studies that have not included intensity as a variable for IHC analysis.
Keywords
O6-Methylguanine-DNA-methyltransferase, Glioblastoma, Staining intensity, Immunohistochemistry
About this articleTitle
O6-methylguanine-DNA-methyltransferase immunostaining intensity in glioblastoma
Journal
Neurologia i Neurochirurgia Polska
Issue
Pages
116-119
Published online
2017-11-04
Page views
258
Article views/downloads
274
DOI
10.1016/j.pjnns.2017.10.014
Bibliographic record
Neurol Neurochir Pol 2018;52(1):116-119.
Keywords
O6-Methylguanine-DNA-methyltransferase
Glioblastoma
Staining intensity
Immunohistochemistry
Authors
Daniel Jiménez
José Manuel Matamala
Alessandra Chiti
Carmen Vergara
Claudia Tissera
Romulo Melo
Luis Cartier
