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Synergistic effect of oxymatrine and 5-fluorouracil on the migratory potential in A549 non-small cell lung cancer cells
, 2, 2, 12, 2
Affiliations- Students research group of Cell Biology and Ultrastructure at Department of Histology and Embryology, Nicolaus Copernicus University in Torun, Collegium Medicum in Bydgoszcz, Poland
- Department of Histology and Embryology, Nicolaus Copernicus University in Torun, Faculty of Medicine, Collegium Medicum in Bydgoszcz, Poland
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Abstract
Introduction: An interesting research direction is the development of new therapies to reduce metastasis, especially in highly invasive cancer such as lung cancer. One of the commonly used anti-cancer drugs is 5-fluorouracil. Oxymatrine is a natural alkaloid with a wide range of effects. Combined with a cytostatic, it may enhance its action and protect normal cells. Therefore, the study aimed to analyse the effect of oxymatrine and 5-fluorouracil on non-small lung cancer cell line A549.
Material and methods: The study was based on the assessment of the interaction between drugs, cell death, cell cycle phase distribution, fluorescent labelling of F-actin and b-catenin, as well as wound healing and transwell migration assay.
Results: The combined treatment with oxymatrine and the cytostatic in a 1:1 ratio resulted in synergism. Incubation of cells with both substances induced changes in the life processes of A549 cells. In turn, the reorganization of F-actin and b-catenin contributed to the limitation of lung cancer cell migration compared to individual treatment with compounds.
Conclusions: This study demonstrated that the combination of oxymatrine and 5-fluorouracil in the 1:1 ratio may limit the migratory potential of A549 cells. In summary, oxymatrine can support the anti-cancer effect of 5-fluorouracil, but its potential application should be examined in further studies.
Abstract
Introduction: An interesting research direction is the development of new therapies to reduce metastasis, especially in highly invasive cancer such as lung cancer. One of the commonly used anti-cancer drugs is 5-fluorouracil. Oxymatrine is a natural alkaloid with a wide range of effects. Combined with a cytostatic, it may enhance its action and protect normal cells. Therefore, the study aimed to analyse the effect of oxymatrine and 5-fluorouracil on non-small lung cancer cell line A549.
Material and methods: The study was based on the assessment of the interaction between drugs, cell death, cell cycle phase distribution, fluorescent labelling of F-actin and b-catenin, as well as wound healing and transwell migration assay.
Results: The combined treatment with oxymatrine and the cytostatic in a 1:1 ratio resulted in synergism. Incubation of cells with both substances induced changes in the life processes of A549 cells. In turn, the reorganization of F-actin and b-catenin contributed to the limitation of lung cancer cell migration compared to individual treatment with compounds.
Conclusions: This study demonstrated that the combination of oxymatrine and 5-fluorouracil in the 1:1 ratio may limit the migratory potential of A549 cells. In summary, oxymatrine can support the anti-cancer effect of 5-fluorouracil, but its potential application should be examined in further studies.
Keywords
Oxymatrine, 5-FU, non-small cell lung cancer, migratory potential
About this articleTitle
Synergistic effect of oxymatrine and 5-fluorouracil on the migratory potential in A549 non-small cell lung cancer cells
Journal
Issue
Article type
Original article
Pages
293-300
Published online
2022-11-29
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3386
Article views/downloads
326
DOI
Bibliographic record
Medical Research Journal 2022;7(4):293-300.
Keywords
Oxymatrine
5-FU
non-small cell lung cancer
migratory potential
Authors
Agnieszka Mlicka
Wioletta Zielińska
Klaudia Mikołajczyk
Marta Hałas-Wiśniewska
Magdalena Izdebska
Alina Grzanka
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