Vol 6, No 1 (2021)
Original article
Published online: 2021-02-08

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Effect of Lactobacillus spp. strains on the population of Listeria monocytogenes isolated from the human vagina

Krzysztof Skowron1, Ewa Wałecka-Zacharska2, Natalia Wiktorczyk-Kapischke1, Katarzyna Grudlewska-Buda1, Zuzanna Bernaciak1, Anna Gralewska1, Eugenia Gospodarek-Komkowska1
Medical Research Journal 2021;6(1):8-15.

Abstract

Introduction: The normal vaginal microbiota (mainly Lactobacillus spp.) affects the health of these areas. Bacterial vaginosis is a serious health problem among many women, especially dangerous for pregnant women. The study aimed to assess the impact of Lactobacillus spp. strains on the population of Listeria monocytogenes isolated from women.

Materials and methods: The research material consisted of reference strains of Lactobacillus spp.: L. acidophilus (LAC), L. fermentum (LFE), L. gasseri (LGA), L. plantarum (LPL), the strain L. monocytogenes ATCC 19111 and 7 L. monocytogenes strains isolated from the vagina.

Results: The highest antagonistic activity was shown for the mixed culture of all Lactobacillus strains (LACTO MIX) used in the experiment. Among the individual strains of Lactobacillus spp. strains, L. plantarum turned out to most effectively reduce L. monocytogenes number (reduction of 5.74 log CFU × ml-1). The least effective in inhibiting the growth of L. monocytogenes was the L. acidophilus strain (reduction of L. monocytogenes of a number of 2.21 log CFU × ml-1).

Conclusions: The presence of Lactobacillus spp. in the genital tract limits the development of bacterial infections, which is an important aspect especially for pregnant women.

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