open access
The influence of house dust extract on normal lung cell Mrc5 and non-small lung carcinoma A549
Affiliations- Department of Histology and Embryology, Nicolas Copernicus University in Toruń, Faculty of Medicine, Collegium Medicum in Bydgoszcz, Poland
- Students research group of Cell Biology and Ultrastructure at Department of Histology and Embryology, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, Poland
- Department of Histology and Embryology, Nicolas Copernicus University in Toruń, Faculty of Medicine, Collegium Medicum in Bydgoszcz, Karłowicza 24, 85-092 Bydgoszcz, Poland
- Students research group of Cell Biology and Ultrastructure at Department of Histology and Embryology, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz,, Karłowicza 24, 85-092 Bydgoszcz, Poland
open access
Abstract
Introduction. Desensitisation is a therapeutic method of allergic disease treatment, but its impact on the cellular level remains to be elucidated. The purpose of this study was to identify the influence of house dust extract on two cell lines types: non-small lung cancer A549 and non-cancerous lung fibroblast Mrc5. Furthermore, we analysed cell viability, type of cell death, and reorganisation of mainly cytoskeletal proteins such as vimentin, F-actin, and b-tubulin. Material and methods. To determine the cell viability, the MTT test was used. The type of cell death was analysed using double staining of annexin V and iodide propidium. The reorganisation of cytoskeletal proteins was evaluated by fluorescent staining and microscopy observation. Results. Our data presented non-statistical differences in a population of live, apoptotic, and necrotic cells. We did not observe significant abnormalities in cytoskeletal reorganisation. Moreover, Mrc5 cell line exhibited a lower sensitivity for house dust extract in comparison to A549 cell line. Conclusions. Our study suggests that desensitisation has no significant influence on survival and cytoskeleton in both cell lines, which correlate with potential use of this method in cancer patients. Obviously, the choice of these kinds of treatment should be used carefully in consultation with a specialist. Additionally, to our knowledge, it was the first presentation of the influence of house dust extract on cells in the context of the main cytoskeletal reorganisations.
Abstract
Introduction. Desensitisation is a therapeutic method of allergic disease treatment, but its impact on the cellular level remains to be elucidated. The purpose of this study was to identify the influence of house dust extract on two cell lines types: non-small lung cancer A549 and non-cancerous lung fibroblast Mrc5. Furthermore, we analysed cell viability, type of cell death, and reorganisation of mainly cytoskeletal proteins such as vimentin, F-actin, and b-tubulin. Material and methods. To determine the cell viability, the MTT test was used. The type of cell death was analysed using double staining of annexin V and iodide propidium. The reorganisation of cytoskeletal proteins was evaluated by fluorescent staining and microscopy observation. Results. Our data presented non-statistical differences in a population of live, apoptotic, and necrotic cells. We did not observe significant abnormalities in cytoskeletal reorganisation. Moreover, Mrc5 cell line exhibited a lower sensitivity for house dust extract in comparison to A549 cell line. Conclusions. Our study suggests that desensitisation has no significant influence on survival and cytoskeleton in both cell lines, which correlate with potential use of this method in cancer patients. Obviously, the choice of these kinds of treatment should be used carefully in consultation with a specialist. Additionally, to our knowledge, it was the first presentation of the influence of house dust extract on cells in the context of the main cytoskeletal reorganisations.
Keywords
house dust extract, cell death, cytoskeleton, A549, Mrc5
About this articleTitle
The influence of house dust extract on normal lung cell Mrc5 and non-small lung carcinoma A549
Journal
Issue
Article type
Original article
Pages
102-110
Published online
2018-01-31
Page views
960
Article views/downloads
1049
DOI
Bibliographic record
Medical Research Journal 2017;2(3):102-110.
Keywords
house dust extract
cell death
cytoskeleton
A549
Mrc5
Authors
Marta Hałas-Wiśniewska *
Wioletta Zielińska*
Karolina Matulewicz
Anna Kokocha
Agnieszka Nawrocka
Maciej Rydzkowski
Iwona Adamczyk
Magdalena Izdebska
Alina Grzanka
References (29)- Butte W, Heinzow B. Pollutants in house dust as indicators of indoor contamination. Rev Environ Contam Toxicol. 2002; 175: 1–46.
- Dekker H. Asthma und Milben. Munchener Medizinische Wochenschrift. 1928; 75: 515.
- Voorhorst R, Spieksma FT. Recent progress in the house dust mite problem. Acta Allergol. 1969; 24(2): 115–123.
- Weber J, Illi S, Nowak D, et al. Asthma and the hygiene hypothesis. Does cleanliness matter? Am J Respir Crit Care Med. 2015; 191(5): 522–529.
- Roberts JW, Wallace LA, Camann DE, et al. Monitoring and reducing exposure of infants to pollutants in house dust. Rev Environ Contam Toxicol. 2009; 201: 1–39.
- Lighty J, Veranth J, Sarofim A. Combustion Aerosols: Factors Governing Their Size and Composition and Implications to Human Health. Journal of the Air & Waste Management Association. 2011; 50(9): 1565–1618.
- Chan TK, Loh XYi, Peh HY, et al. House dust mite-induced asthma causes oxidative damage and DNA double-strand breaks in the lungs. J Allergy Clin Immunol. 2016; 138(1): 84–96.e1.
- Murray AB, Ferguson AC. Dust-free bedrooms in the treatment of asthmatic children with house dust or house dust mite allergy: a controlled trial. Pediatrics. 1983; 71(3): 418–422.
- Burr ML, St Leger AS, Neale E. Anti-mite measurements in mite-sensitive adult asthma. A controlled trial. Lancet. 1976; 1(7955): 333–335.
- Burr ML, Dean BV, Merrett TG, et al. Effects of anti-mite measures on children with mite-sensitive asthma: a controlled trial. Thorax. 1980; 35(7): 506–512.
- Klimaszewska-Wiśniewska A, Hałas-Wiśniewska M, Izdebska M, et al. Antiproliferative and antimetastatic action of quercetin on A549 non-small cell lung cancer cells through its effect on the cytoskeleton. Acta Histochem. 2017; 119(2): 99–112.
- Arlian LG, Morgan MS, Peterson KT. House dust and storage mite extracts influence skin keratinocyte and fibroblast function. Int Arch Allergy Immunol. 2008; 145(1): 33–42.
- Xiang P, He RW, Han YH, et al. Mechanisms of housedust-induced toxicity in primary human corneal epithelial cells: Oxidative stress, proinflammatory response and mitochondrial dysfunction. Environ Int. 2016; 89-90: 30–37.
- King C, Brennan S, Thompson PJ, et al. Dust mite proteolytic allergens induce cytokine release from cultured airway epithelium. J Immunol. 1998; 161(7): 3645–3651.
- Kang Y, Cheung KC, Wong MH. The use of cytokine array to examine cytokine profiles of two human cell lines exposed to indoor dust. Toxicol Lett. 2010; 199(3): 301–307.
- Asokananthan N, Graham PT, Stewart DJ, et al. House dust mite allergens induce proinflammatory cytokines from respiratory epithelial cells: the cysteine protease allergen, Der p 1, activates protease-activated receptor (PAR)-2 and inactivates PAR-1. J Immunol. 2002; 169(8): 4572–4578.
- Rockwood J, Morgan MS, Arlian LG. Proteins and endotoxin in house dust mite extracts modulate cytokine secretion and gene expression by dermal fibroblasts. Exp Appl Acarol. 2013; 61(3): 311–325.
- Wong CK, Li MLY, Wang CB, et al. House dust mite allergen Der p 1 elevates the release of inflammatory cytokines and expression of adhesion molecules in co-culture of human eosinophils and bronchial epithelial cells. Int Immunol. 2006; 18(8): 1327–1335.
- Mascia F, Mariani V, Giannetti A, et al. House dust mite allergen exerts no direct proinflammatory effects on human keratinocytes. J Allergy Clin Immunol. 2002; 109(3): 532–538.
- Arlian LG, Elder BL, Morgan MS. House dust mite extracts activate cultured human dermal endothelial cells to express adhesion molecules and secrete cytokines. J Med Entomol. 2009; 46(3): 595–604.
- Osterlund C, Grönlund H, Polovic N, et al. The non-proteolytic house dust mite allergen Der p 2 induce NF-kappaB and MAPK dependent activation of bronchial epithelial cells. Clin Exp Allergy. 2009; 39(8): 1199–1208.
- Bousquet J, Lockey RF, Malling H-J, et al. Immunoterapia alergenami: szczepionki terapeutyczne w chorobach alergicznych cz.I. Stanowisko Światowej Organizacji ZdrowiaAlergia Astma Immunologia. 2000; 5(1): 7-30 PMID. : 9860031.
- Calderón MA, Kleine-Tebbe J, Linneberg A, et al. House Dust Mite Respiratory Allergy: An Overview of Current Therapeutic Strategies. J Allergy Clin Immunol Pract. 2015; 3(6): 843–855.
- Fletcher DA, Mullins RD. Cell mechanics and the cytoskeleton. Nature. 2010; 463(7280): 485–492.
- Etienne-Manneville S. Actin and microtubules in cell motility: which one is in control? Traffic. 2004; 5(7): 470–477.
- Ivaska J, Pallari HM, Nevo J, et al. Novel functions of vimentin in cell adhesion, migration, and signaling. Exp Cell Res. 2007; 313(10): 2050–2062.
- Pollard TD. The cytoskeleton, cellular motility and the reductionist agenda. Nature. 2003; 422(6933): 741–745.
- Fischer K, Agrawal D. Induction of Epithelial-Mesenchymal Transition in House Dust Mite, Ragweed, and Alternaria Sensitized and Challenged Mice. Journal of Allergy and Clinical Immunology. 2015; 135(2): AB62.
- Peterson LJ, Rajfur Z, Maddox AS, et al. Simultaneous stretching and contraction of stress fibers in vivo. Mol Biol Cell. 2004; 15(7): 3497–3508.