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Effect of miR-195 inhibition on human skeletal muscle-derived stem/progenitor cells

Magdalena Nowaczyk1, Agnieszka Malcher1, Agnieszka Zimna1, Natalia Rozwadowska1, Maciej Kurpisz1
DOI: 10.33963/KP.a2022.0127
·
Pubmed: 35554929
Affiliations
  1. Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland

open access

Online first
Original article
Published online: 2022-05-13

Abstract

BACKGROUND: Application of a circulating miR-195 inhibitor could be a helping factor in in vitro model of human skeletal muscle- derived stem/progenitor cells (SkMDS/PCs). Previously, miR-195 expression has been reported to be a negative factor for myogenesis.

AIMS: The aim of the study was to obtain anti-apoptotic and anti-aging effects in in vitro cultured myoblasts and to improve their ability to form myotubes by suppressing miR-195 expression.

METHODS: Human wild-type (WT) SkMDS/PC cells incubated with control (nonspecific) miRNA inhibitor and miR-195-inhibited SkMDS/PCs were studied. Functional assays (myotube formation and cell ageing), antioxidant, and myogenic gene expression analyses were performed at two time points, at the 7th and 11th cell passages.

RESULTS: Myotube formation was found to be almost 2-fold higher in the miR-195-inhibited SkMDS/PCs population (p<0.05) compared to WT cells. miR-195 inhibition did not appear to affect cell ageing or rejuvenate human SkMDS/PCs. Antioxidant (SOD3 and FOXO) gene expression was augmented in the miR-195-inhibited SkMDS/PCs population, but no positive effect on the remaining antioxidant genes (SOD1, SOD2, and catalase) was observed. A significant increase in MyoD gene expression with a concomitant decrease in MyoG (p<0.05) was further documented in miR-195-inhibited SkMDS/PCs compared to WT cells (11th cell passage).

CONCLUSIONS: The performed studies may lead to the preconditioning of myogenic stem cells to extend their potential for pro-regenerative activity. miR-195 inhibitor may serve as conditioning factor augmenting selective antioxidant genes expression and proliferative potential of SkMDS/PCs, but not having an impact on cell aging and/ or apoptosis.

Abstract

BACKGROUND: Application of a circulating miR-195 inhibitor could be a helping factor in in vitro model of human skeletal muscle- derived stem/progenitor cells (SkMDS/PCs). Previously, miR-195 expression has been reported to be a negative factor for myogenesis.

AIMS: The aim of the study was to obtain anti-apoptotic and anti-aging effects in in vitro cultured myoblasts and to improve their ability to form myotubes by suppressing miR-195 expression.

METHODS: Human wild-type (WT) SkMDS/PC cells incubated with control (nonspecific) miRNA inhibitor and miR-195-inhibited SkMDS/PCs were studied. Functional assays (myotube formation and cell ageing), antioxidant, and myogenic gene expression analyses were performed at two time points, at the 7th and 11th cell passages.

RESULTS: Myotube formation was found to be almost 2-fold higher in the miR-195-inhibited SkMDS/PCs population (p<0.05) compared to WT cells. miR-195 inhibition did not appear to affect cell ageing or rejuvenate human SkMDS/PCs. Antioxidant (SOD3 and FOXO) gene expression was augmented in the miR-195-inhibited SkMDS/PCs population, but no positive effect on the remaining antioxidant genes (SOD1, SOD2, and catalase) was observed. A significant increase in MyoD gene expression with a concomitant decrease in MyoG (p<0.05) was further documented in miR-195-inhibited SkMDS/PCs compared to WT cells (11th cell passage).

CONCLUSIONS: The performed studies may lead to the preconditioning of myogenic stem cells to extend their potential for pro-regenerative activity. miR-195 inhibitor may serve as conditioning factor augmenting selective antioxidant genes expression and proliferative potential of SkMDS/PCs, but not having an impact on cell aging and/ or apoptosis.

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Keywords

miR-195 inhibitor, human skeletal muscle-derived stem/progenitor cells (SkMDS/PCs), apoptosis, oxidative stress, regenerative medicine

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Title

Effect of miR-195 inhibition on human skeletal muscle-derived stem/progenitor cells

Journal

Kardiologia Polska (Polish Heart Journal)

Issue

Online first

Article type

Original article

Published online

2022-05-13

Page views

9

Article views/downloads

6

DOI

10.33963/KP.a2022.0127

Pubmed

35554929

Keywords

miR-195 inhibitor
human skeletal muscle-derived stem/progenitor cells (SkMDS/PCs)
apoptosis
oxidative stress
regenerative medicine

Authors

Magdalena Nowaczyk
Agnieszka Malcher
Agnieszka Zimna
Natalia Rozwadowska
Maciej Kurpisz

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