open access

Vol 12, No 4 (2019)
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Molecular biology methods for blood cell antigen genotyping in reference laboratories

Katarzyna Guz, Agnieszka Orzińska, Bogumiła Michalewska, Monika Pelc-Kłopotowska, Ewa Brojer, Magdalena Łętowska
DOI: 10.5603/JTM.2019.0010
·
Journal of Transfusion Medicine 2019;12(4):199-205.

open access

Vol 12, No 4 (2019)
REVIEWS

Abstract

The aim of the study is to present current applications of molecular biology methods in immunohematology
as well as the status of their implementation in the Polish Blood Transfusion Service. Molecular biology methods are used for: analysis of molecular background of blood cell antigens in individuals with discrepant results of serological tests, identification of RhD antigen with weak expression undetectable by serological methods in blood donors, identification of weak D type antigen in recipients unsusceptible to alloimmunization. These methods are also used for noninvasive study of fetal genes in plasma samples of immunized pregnant women with serological incompatibilities and in RhD negative pregnant women for qualification to antenatal prophylactic administration of anti-D immunoglobulin. Molecular biology is also an important tool for diagnostics of patients with antibodies to high-frequency blood group antigens (HFA) or for patients immunized with HPA and HNA antigens and for identification of “antigen-negative” blood donors for such patients. In Poland, all these tests are available and in use.

Abstract

The aim of the study is to present current applications of molecular biology methods in immunohematology
as well as the status of their implementation in the Polish Blood Transfusion Service. Molecular biology methods are used for: analysis of molecular background of blood cell antigens in individuals with discrepant results of serological tests, identification of RhD antigen with weak expression undetectable by serological methods in blood donors, identification of weak D type antigen in recipients unsusceptible to alloimmunization. These methods are also used for noninvasive study of fetal genes in plasma samples of immunized pregnant women with serological incompatibilities and in RhD negative pregnant women for qualification to antenatal prophylactic administration of anti-D immunoglobulin. Molecular biology is also an important tool for diagnostics of patients with antibodies to high-frequency blood group antigens (HFA) or for patients immunized with HPA and HNA antigens and for identification of “antigen-negative” blood donors for such patients. In Poland, all these tests are available and in use.

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Keywords

molecular methods for genotyping; blood group antigens; platelet antigens (HPA); granulocyte antigens (HNA); immunohematological studies

About this article
Title

Molecular biology methods for blood cell antigen genotyping in reference laboratories

Journal

Journal of Transfusion Medicine

Issue

Vol 12, No 4 (2019)

Pages

199-205

DOI

10.5603/JTM.2019.0010

Bibliographic record

Journal of Transfusion Medicine 2019;12(4):199-205.

Keywords

molecular methods for genotyping
blood group antigens
platelet antigens (HPA)
granulocyte antigens (HNA)
immunohematological studies

Authors

Katarzyna Guz
Agnieszka Orzińska
Bogumiła Michalewska
Monika Pelc-Kłopotowska
Ewa Brojer
Magdalena Łętowska

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