open access

Vol 3, No 2 (2010)
Research paper
Published online: 2010-06-02
Get Citation

Pooled platelet concentrates obtained with automatic method

Jolanta Antoniewicz-Papis, Elżbieta Lachert, Aleksandra Rosiek, Jolanta Kubis, Karolina Janik, Dariusz Piotrowski, Magdalena Łętowska
Journal of Transfusion Medicine 2010;3(2):49-54.

open access

Vol 3, No 2 (2010)
ORIGINAL PAPERS
Published online: 2010-06-02

Abstract

Background: Platelet Concentrates (PCs) may be obtained by conventional methods from whole blood or by apheresis. Buffy-coat pooling from whole blood by manual method is time consuming and requires a variety of special equipment and qualified staff. An alternative is automatic buffy coat pooling which simplifies the procedure and helps to eliminate human errors. The aim of our study was to evaluate the qualitative and quantitative parameters of pooled, leukoreduced PCs obtained from buffy coats with the OrbiSac (Caridian) automatic pooling system.
Material and methods: Buffy coats were prepared in the OrbiSac system to obtain pooled leukoreduced PCs, the quality of which was immediately evaluated and then assessed during 5 days of storage. The following parameters were monitored: platelet count, leukocyte count, CD42b and CD62 antigen expression, hypotonic shock response and pH.
Results: We obtained ten leukoreduced PCs (mean volume 315 ml) with platelet count as required. During storage, the PC hypotonic shock response was high as was the CD42b antigen expression with a slight decreasing tendency; CD62 antigen expression first indicated decrease (storage day 3) then increase (storage day 5). Throughout the storage period the pH level was as required.
Conclusions: Leukoreduced PCs obtained from buffy coats with the OrbiSac (Caridian) system are high quality with parameters consistent with required values. Undisputable advantages of this automatic method is the elimination of human errors and method standardization.
J. Transf. Med. 2010; 2: 49-54

Abstract

Background: Platelet Concentrates (PCs) may be obtained by conventional methods from whole blood or by apheresis. Buffy-coat pooling from whole blood by manual method is time consuming and requires a variety of special equipment and qualified staff. An alternative is automatic buffy coat pooling which simplifies the procedure and helps to eliminate human errors. The aim of our study was to evaluate the qualitative and quantitative parameters of pooled, leukoreduced PCs obtained from buffy coats with the OrbiSac (Caridian) automatic pooling system.
Material and methods: Buffy coats were prepared in the OrbiSac system to obtain pooled leukoreduced PCs, the quality of which was immediately evaluated and then assessed during 5 days of storage. The following parameters were monitored: platelet count, leukocyte count, CD42b and CD62 antigen expression, hypotonic shock response and pH.
Results: We obtained ten leukoreduced PCs (mean volume 315 ml) with platelet count as required. During storage, the PC hypotonic shock response was high as was the CD42b antigen expression with a slight decreasing tendency; CD62 antigen expression first indicated decrease (storage day 3) then increase (storage day 5). Throughout the storage period the pH level was as required.
Conclusions: Leukoreduced PCs obtained from buffy coats with the OrbiSac (Caridian) system are high quality with parameters consistent with required values. Undisputable advantages of this automatic method is the elimination of human errors and method standardization.
J. Transf. Med. 2010; 2: 49-54
Get Citation

Keywords

platelet concentrates; platelet activation; platelet count; leukocyte reducing procedure

About this article
Title

Pooled platelet concentrates obtained with automatic method

Journal

Journal of Transfusion Medicine

Issue

Vol 3, No 2 (2010)

Article type

Research paper

Pages

49-54

Published online

2010-06-02

Bibliographic record

Journal of Transfusion Medicine 2010;3(2):49-54.

Keywords

platelet concentrates
platelet activation
platelet count
leukocyte reducing procedure

Authors

Jolanta Antoniewicz-Papis
Elżbieta Lachert
Aleksandra Rosiek
Jolanta Kubis
Karolina Janik
Dariusz Piotrowski
Magdalena Łętowska

References (12)
  1. Łętowska M, Sabliński J, Sharf R, Seyfried H. Leczenie krwią. Zasady postępowania klinicznego. Sekcja Transfuzjologiczna PTHiT, Warszawa 2001.
  2. Menichella G, Serafini R, Ciarli M, et al. A new blood donation strategy: automated blood collection (ABC). Int J Artif Organs. 2001; 24(3): 173–177.
  3. Rock G, Moltzan C, Alharbi A, et al. Automated collection of blood components: their storage and transfusion. Transfus Med. 2003; 13(4): 219–225.
  4. Janetzko K, Klüter H, van Waeg G, et al. Fully automated processing of buffy-coat-derived pooled platelet concentrates. Transfusion. 2004; 44(7): 1052–1058.
  5. Larsson S, Sandgren P, Sjödin A, et al. Automated preparation of platelet concentrates from pooled buffy coats: in vitro studies and experiences with the OrbiSac system. Transfusion. 2005; 45(5): 743–751.
  6. Saekmose S, Homburg K, Albjerg L. Evaluating production of platelet concentrates using the OrbiSac centrifuge. Vox Sanguinis. 2004; 87: S93–S145.
  7. Santos M, Ferreira AR, Sousa G. Automated processing of platelet concentrates using OrbiSac system. Vox Sanguinis. 2006; 91(supl. 3): P724.
  8. Curvers J, Heermans J. Responsiveness of pooled platelets produced by the OrbiSac BC System and stored in different media centrifuge. Vox Sanguinis. 2004; 87: S93–S145.
  9. Laupacis A, Brown J, Costello B, et al. Prevention of posttransfusion CMV in the era of universal WBC reduction: a consensus statement. Transfusion. 2001; 41(4): 560–569.
  10. Łętowska M. Medyczne zasady pobierania krwi, oddzielania jej składników i wydawania, obowiązujące w jednostkach organizacyjnych publicznej służby krwi. , Warszawa 2009.
  11. Murphy S. Platelets from pooled buffy coats: an update. Transfusion. 2005; 45(4): 634–639.
  12. Vetlesen A, Mirlashari MR, Ezligini F, et al. Evaluation of platelet activation and cytokine release during storage of platelet concentrates processed from buffy coats either manually or by the automated OrbiSac system. Transfusion. 2007; 47(1): 126–132.

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

Czasopismo Journal of Transfusion Medicine dostęne jest również w Ikamed - księgarnia medyczna

Wydawcą serwisu jest Via Medica sp. z o.o. sp. komandytowa, ul. Świętokrzyska 73, 80–180 Gdańsk

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail: viamedica@viamedica.pl