English Polski
Vol 3, No 2 (2010)
Research paper
Published online: 2010-06-02

open access

Page views 1010
Article views/downloads 3453
Get Citation

Connect on Social Media

Connect on Social Media

Pooled platelet concentrates obtained with automatic method

Jolanta Antoniewicz-Papis, Elżbieta Lachert, Aleksandra Rosiek, Jolanta Kubis, Karolina Janik, Dariusz Piotrowski, Magdalena Łętowska
Journal of Transfusion Medicine 2010;3(2):49-54.


Background: Platelet Concentrates (PCs) may be obtained by conventional methods from whole blood or by apheresis. Buffy-coat pooling from whole blood by manual method is time consuming and requires a variety of special equipment and qualified staff. An alternative is automatic buffy coat pooling which simplifies the procedure and helps to eliminate human errors. The aim of our study was to evaluate the qualitative and quantitative parameters of pooled, leukoreduced PCs obtained from buffy coats with the OrbiSac (Caridian) automatic pooling system.
Material and methods: Buffy coats were prepared in the OrbiSac system to obtain pooled leukoreduced PCs, the quality of which was immediately evaluated and then assessed during 5 days of storage. The following parameters were monitored: platelet count, leukocyte count, CD42b and CD62 antigen expression, hypotonic shock response and pH.
Results: We obtained ten leukoreduced PCs (mean volume 315 ml) with platelet count as required. During storage, the PC hypotonic shock response was high as was the CD42b antigen expression with a slight decreasing tendency; CD62 antigen expression first indicated decrease (storage day 3) then increase (storage day 5). Throughout the storage period the pH level was as required.
Conclusions: Leukoreduced PCs obtained from buffy coats with the OrbiSac (Caridian) system are high quality with parameters consistent with required values. Undisputable advantages of this automatic method is the elimination of human errors and method standardization.
J. Transf. Med. 2010; 2: 49-54

Article available in PDF format

View PDF (Polish) Download PDF file


  1. Łętowska M, Sabliński J, Sharf R, Seyfried H. Leczenie krwią. Zasady postępowania klinicznego. Sekcja Transfuzjologiczna PTHiT, Warszawa 2001.
  2. Menichella G, Serafini R, Ciarli M, et al. A new blood donation strategy: automated blood collection (ABC). Int J Artif Organs. 2001; 24(3): 173–177.
  3. Rock G, Moltzan C, Alharbi A, et al. Automated collection of blood components: their storage and transfusion. Transfus Med. 2003; 13(4): 219–225.
  4. Janetzko K, Klüter H, van Waeg G, et al. Fully automated processing of buffy-coat-derived pooled platelet concentrates. Transfusion. 2004; 44(7): 1052–1058.
  5. Larsson S, Sandgren P, Sjödin A, et al. Automated preparation of platelet concentrates from pooled buffy coats: in vitro studies and experiences with the OrbiSac system. Transfusion. 2005; 45(5): 743–751.
  6. Saekmose S, Homburg K, Albjerg L. Evaluating production of platelet concentrates using the OrbiSac centrifuge. Vox Sanguinis. 2004; 87: S93–S145.
  7. Santos M, Ferreira AR, Sousa G. Automated processing of platelet concentrates using OrbiSac system. Vox Sanguinis. 2006; 91(supl. 3): P724.
  8. Curvers J, Heermans J. Responsiveness of pooled platelets produced by the OrbiSac BC System and stored in different media centrifuge. Vox Sanguinis. 2004; 87: S93–S145.
  9. Laupacis A, Brown J, Costello B, et al. Prevention of posttransfusion CMV in the era of universal WBC reduction: a consensus statement. Transfusion. 2001; 41(4): 560–569.
  10. Łętowska M. Medyczne zasady pobierania krwi, oddzielania jej składników i wydawania, obowiązujące w jednostkach organizacyjnych publicznej służby krwi. , Warszawa 2009.
  11. Murphy S. Platelets from pooled buffy coats: an update. Transfusion. 2005; 45(4): 634–639.
  12. Vetlesen A, Mirlashari MR, Ezligini F, et al. Evaluation of platelet activation and cytokine release during storage of platelet concentrates processed from buffy coats either manually or by the automated OrbiSac system. Transfusion. 2007; 47(1): 126–132.