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Vol 89, No 4 (2018)
Research paper
Published online: 2018-04-30
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Wound fluids collected from patients after IORT treatment activates extrinsic apoptotic pathway in MCF7 breast cancer cell line

Katarzyna Ida Kulcenty12, Igor Piotrowski12, Karolina Zaleska1, Dawid Murawa, Wiktoria Maria Suchorska12
DOI: 10.5603/GP.a2018.0030
·
Pubmed: 29781071
·
Ginekol Pol 2018;89(4):175-182.
Affiliations
  1. Radiobiology Labolatory, Department of Medical Physics, Greater Poland Cancer Centre, Garbary 15 Street, 61-866 Poznań, Poland
  2. Department of Electroradiology, University of Medical Sciences, Poznan, Poland, Garbary 15 Street, 61-866 Poznań, Poland

open access

Vol 89, No 4 (2018)
ORIGINAL PAPERS Gynecology
Published online: 2018-04-30

Abstract

Objectives: Intraoperative radiotherapy (IORT) relates to irradiation of diseased tissue during the surgery within the tumor bed. The reason for this process is based on the fact that the increase in the radiation dose increases local tumor control. It was shown that postoperative fluids obtained from patients after breast cancer conserving surgery, stimulated motility and invasiveness of tumor cells in vitro. The results obtained from TARGIT clinical trial demonstrated that IORT significantly inhibits the stimulatory effect of wound fluids on tumor cells in vitro. We therefore speculated that wound fluids collected from patients after IORT treatment may induce the apoptosis in breast cancer cell lines and it may be a reason for their lower proliferation rate and potential to metastasis.

Material and methods: Breast cancer MCF7 cell line was incubated with wound fluids collected from patients after conserving breast cancer surgery or surgery followed by IORT for 4 days. Then the expression of markers associated with extrinsic or intrinsic apoptosis pathway was established.

Results: Our results clearly indicate activation of extrinsic apoptosis pathway by wound fluids collected from patients after IORT treatment. No changes in apoptotic markers were seen in cells treated with wound fluids collected from patients after the surgery alone.

Conclusions: Thus we confirmed that wound fluids collected from patients after IORT treatment may induce the apoptosis in breast cancer cell lines and it may be a reason for their lower proliferation rate and invasiveness of tumor cells in vitro.

Abstract

Objectives: Intraoperative radiotherapy (IORT) relates to irradiation of diseased tissue during the surgery within the tumor bed. The reason for this process is based on the fact that the increase in the radiation dose increases local tumor control. It was shown that postoperative fluids obtained from patients after breast cancer conserving surgery, stimulated motility and invasiveness of tumor cells in vitro. The results obtained from TARGIT clinical trial demonstrated that IORT significantly inhibits the stimulatory effect of wound fluids on tumor cells in vitro. We therefore speculated that wound fluids collected from patients after IORT treatment may induce the apoptosis in breast cancer cell lines and it may be a reason for their lower proliferation rate and potential to metastasis.

Material and methods: Breast cancer MCF7 cell line was incubated with wound fluids collected from patients after conserving breast cancer surgery or surgery followed by IORT for 4 days. Then the expression of markers associated with extrinsic or intrinsic apoptosis pathway was established.

Results: Our results clearly indicate activation of extrinsic apoptosis pathway by wound fluids collected from patients after IORT treatment. No changes in apoptotic markers were seen in cells treated with wound fluids collected from patients after the surgery alone.

Conclusions: Thus we confirmed that wound fluids collected from patients after IORT treatment may induce the apoptosis in breast cancer cell lines and it may be a reason for their lower proliferation rate and invasiveness of tumor cells in vitro.

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Keywords

apoptosis, wound fluids, intraoperative radiation therapy, breast cancer

About this article
Title

Wound fluids collected from patients after IORT treatment activates extrinsic apoptotic pathway in MCF7 breast cancer cell line

Journal

Ginekologia Polska

Issue

Vol 89, No 4 (2018)

Article type

Research paper

Pages

175-182

Published online

2018-04-30

Page views

1444

Article views/downloads

1201

DOI

10.5603/GP.a2018.0030

Pubmed

29781071

Bibliographic record

Ginekol Pol 2018;89(4):175-182.

Keywords

apoptosis
wound fluids
intraoperative radiation therapy
breast cancer

Authors

Katarzyna Ida Kulcenty
Igor Piotrowski
Karolina Zaleska
Dawid Murawa
Wiktoria Maria Suchorska

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