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ICTP concentration in cervical-vaginal fluid as a potential marker of membrane collagen degradation before labor
, 1, 1
Affiliations- Jagiellonian University Medical College, Department of Obstetrics and Perinatology, Cracow, Poland, Poland
- ”Ujastek” Obstetrics-Gynecological Hospital, Cracow, Poland, Poland
- Jagiellonian University Medical College, Department of Nursing Management and Epidemiology Nursing, Institute of Nursing and Midwifery Faculty of Health Sciences, Cracow, Poland, Poland
- Jagiellonian University Medical College, Department of Endocrynological Gynecology, Cracow, Poland, Poland
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Abstract
Objectives: Numerous physical and chemical processes lead to rupture of membranes. Within the fetal membranes there are numerous types of metalloproteinases, which cause collagen type I degradation. The C-terminal telopeptide of colagen type I (ICTP) is the breakdown product of type I collagen. The aim of the study was to determine whether ICTP is secreted into the vaginal-cervical fluid (VCF) in the case of physiological rupture of the membranes of the fetus before delivery.
Material and methods: The study was conducted in March 2021 at the Department of Obstetrics and Perinatology of the Jagiellonian University in Cracow, Poland. Twenty-three cases were included in the study. During routine gynecological examination with the use of specula, VCF was collected twice in a volume of 50 µL. The obtained material was then subjected to enzyme immunoassay using the Human C-telopeptide of type I collagen (ICTP) ELISA Kit (Catalog Number. CSB-E10363h). The concentration of ICTP in the sample was calibrated. The concentration range that the device can detect was 25 ng /mL–800 ng/mL.
Results: The presence of ICTP in the VCF was confirmed. The minimum concentration was 43.72 ng/mL, the maximum was 762.59, in five cases the concentration was outside the maximum scale of the device.
Conclusions: ICTP was confirmed in the VCF of pregnant women before physiological delivery. Further studies are required to accurately evaluate ICTP as a marker of the processes of collagen degradation in fetal membranes in the mechanism of physiological labor and premature rupture of the membranes.
Abstract
Objectives: Numerous physical and chemical processes lead to rupture of membranes. Within the fetal membranes there are numerous types of metalloproteinases, which cause collagen type I degradation. The C-terminal telopeptide of colagen type I (ICTP) is the breakdown product of type I collagen. The aim of the study was to determine whether ICTP is secreted into the vaginal-cervical fluid (VCF) in the case of physiological rupture of the membranes of the fetus before delivery.
Material and methods: The study was conducted in March 2021 at the Department of Obstetrics and Perinatology of the Jagiellonian University in Cracow, Poland. Twenty-three cases were included in the study. During routine gynecological examination with the use of specula, VCF was collected twice in a volume of 50 µL. The obtained material was then subjected to enzyme immunoassay using the Human C-telopeptide of type I collagen (ICTP) ELISA Kit (Catalog Number. CSB-E10363h). The concentration of ICTP in the sample was calibrated. The concentration range that the device can detect was 25 ng /mL–800 ng/mL.
Results: The presence of ICTP in the VCF was confirmed. The minimum concentration was 43.72 ng/mL, the maximum was 762.59, in five cases the concentration was outside the maximum scale of the device.
Conclusions: ICTP was confirmed in the VCF of pregnant women before physiological delivery. Further studies are required to accurately evaluate ICTP as a marker of the processes of collagen degradation in fetal membranes in the mechanism of physiological labor and premature rupture of the membranes.
Keywords
ICTP; pPROM; rupture of membranes; preterm delivery
About this articleTitle
ICTP concentration in cervical-vaginal fluid as a potential marker of membrane collagen degradation before labor
Journal
Issue
Article type
Research paper
Published online
2023-03-17
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366
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241
DOI
Pubmed
Keywords
ICTP
pPROM
rupture of membranes
preterm delivery
Authors
Magdalena Kolak
Joanna Gorecka
Malgorzata Radon-Pokracka
Maciej Piasecki
Agnieszka Cierniak
Agnieszka Micek
Anna Horbaczewska
Andrzej Jaworowski
Hubert Huras
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