open access

Vol 94, No 6 (2023)
Research paper
Published online: 2022-06-22
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Investigation of serum enzyme activities and oxidative stress markers in preeclampsia: a multiparameter analysis

Sahabettin Selek1, Ayse Zehra Gul1, Nil Atakul2, Sedat Meydan3, Alime Sarıkaya14, Fatmanur Koktasoglu1, Metin Demirel14
·
Pubmed: 36861896
·
Ginekol Pol 2023;94(6):442-450.
Affiliations
  1. Department of Medical Biochemistry, Bezmialem Vakıf University Faculty of Medicine, Istanbul, Turkey, Türkiye
  2. Department of Gynecology and Obstetrics, Istanbul Education Research Hospital, Istanbul, Turkey, Türkiye
  3. Department of Medical Anatomy, Bezmialem Vakıf University Faculty of Medicine, Istanbul, Turkey
  4. Department of Medical Biochemistry, Institute of Health Sciences, Bezmialem Vakif University School of Medicine, Istanbul, Turkey

open access

Vol 94, No 6 (2023)
ORIGINAL PAPERS Gynecology
Published online: 2022-06-22

Abstract

Objectives: Preeclampsia, a high cause of fetomaternal morbidity-mortality, remains a significant burden affecting 8% of all pregnancies. Environmental conditions induce disease development leading to endothelial dysfunction in genetically predisposed women. Our aim is to discuss oxidative stress as a well-established contributing factor to disease progression with being the first study to show new evidence about serum dehydrogenase enzyme levels (isocitrate, malate, glutamate dehydrogenase) with oxidative markers (myeloperoxidase, total antioxidant-oxidant status, oxidative stress index). Material and Methods: Serum parameters were analyzed with photometric method (Abbott ARCHITECT c8000). Results: The enzyme levels and oxidative markers were significantly higher in patients, supporting the redox imbalance in preeclampsia. According to ROC analysis, malate dehydrogenase showed an outstanding diagnostic ability with the highest AUC value of 0.9 and the cut-off value of 51.2 IU/L. Discriminant analysis including malate, isocitrate and glutamate dehydrogenase had predicted preeclampsia with an overall 87.9% accuracy. Conclusions: Considering the above results, we propose that the enzyme levels increase with oxidative stress functioning as antioxidant defense factors. The unique finding of the study is that the serum levels of malate, isocitrate and glutamate dehydrogenase can be used both separately and combined in the early prediction of preeclampsia. As a novel approach, we also offer combining serum isocitrate and glutamate dehydrogenase levels with ALT, AST tests to state liver functions more reliably in patients. Still, larger sample-sized studies investigating enzyme expression levels are required to confirm the recent findings and to reveal underlying mechanisms.

Abstract

Objectives: Preeclampsia, a high cause of fetomaternal morbidity-mortality, remains a significant burden affecting 8% of all pregnancies. Environmental conditions induce disease development leading to endothelial dysfunction in genetically predisposed women. Our aim is to discuss oxidative stress as a well-established contributing factor to disease progression with being the first study to show new evidence about serum dehydrogenase enzyme levels (isocitrate, malate, glutamate dehydrogenase) with oxidative markers (myeloperoxidase, total antioxidant-oxidant status, oxidative stress index). Material and Methods: Serum parameters were analyzed with photometric method (Abbott ARCHITECT c8000). Results: The enzyme levels and oxidative markers were significantly higher in patients, supporting the redox imbalance in preeclampsia. According to ROC analysis, malate dehydrogenase showed an outstanding diagnostic ability with the highest AUC value of 0.9 and the cut-off value of 51.2 IU/L. Discriminant analysis including malate, isocitrate and glutamate dehydrogenase had predicted preeclampsia with an overall 87.9% accuracy. Conclusions: Considering the above results, we propose that the enzyme levels increase with oxidative stress functioning as antioxidant defense factors. The unique finding of the study is that the serum levels of malate, isocitrate and glutamate dehydrogenase can be used both separately and combined in the early prediction of preeclampsia. As a novel approach, we also offer combining serum isocitrate and glutamate dehydrogenase levels with ALT, AST tests to state liver functions more reliably in patients. Still, larger sample-sized studies investigating enzyme expression levels are required to confirm the recent findings and to reveal underlying mechanisms.

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Keywords

isocitrate dehydrogenase; malate dehydrogenase; glutamate dehydrogenase; oxidative stress; preeclampsia

About this article
Title

Investigation of serum enzyme activities and oxidative stress markers in preeclampsia: a multiparameter analysis

Journal

Ginekologia Polska

Issue

Vol 94, No 6 (2023)

Article type

Research paper

Pages

442-450

Published online

2022-06-22

Page views

1605

Article views/downloads

481

DOI

10.5603/GP.a2022.0062

Pubmed

36861896

Bibliographic record

Ginekol Pol 2023;94(6):442-450.

Keywords

isocitrate dehydrogenase
malate dehydrogenase
glutamate dehydrogenase
oxidative stress
preeclampsia

Authors

Sahabettin Selek
Ayse Zehra Gul
Nil Atakul
Sedat Meydan
Alime Sarıkaya
Fatmanur Koktasoglu
Metin Demirel

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