open access

Vol 93, No 4 (2022)
Research paper
Published online: 2022-03-30
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Comparison of MED 12 gene mutation and microRNA-124 expression in leiomyoma and myometrium of Turkish patients

Cagdas Demiroglu1, Ozge Komurcu Karuserci2, Sadrettin Pence3, Halime Hanım Pence3, Burcu Çaykara3, Seyhun Sucu2, Mete Gürol Ugur2
·
Pubmed: 35419790
·
Ginekol Pol 2022;93(4):290-295.
Affiliations
  1. SANKO University, Gaziantep Gazi Muhtarpaşa Bulvarı, Gaziantep, Turkey
  2. Deparment of Obstetrics and Gynecology, Gaziantep Univercity, Medical Medicine, Turkey
  3. Department of Biochemistry, Health Science University,Bakırkoy Sadi Konuk, Training and Research Hospital, Istanbul, Turkey

open access

Vol 93, No 4 (2022)
ORIGINAL PAPERS Gynecology
Published online: 2022-03-30

Abstract

Objectives: It is believed that there are still unclear areas in the formation mechanism of leiomyomas. In our study, it
was aimed to investigate the formation mechanisms of leiomyomas due to local MED 12 gene exon 2 mutation and local
microRNA-124 expression in a Turkish population.
Material and methods: Thirty patients who underwent hysterectomy for leiomyoma uteri at Gaziantep University between
January 2013 and January 2016 were included in our study. In the pathology specimens of these patients, the patient’s
myometrium tissue and her own leiomyoma tissue were analysed via quantitative Realtime PCR in association with MED
12 exon 2 mutation and microRNA-124 expression.
Results: The average age of the 30 patients included in our study is 46.67 ± 5.42 and 13 patients had single leiomyoma;
17 patients had more than one leiomyoma. There were significantly higher c.130G> T (p.G44C) mutation and c.131G> A
(p.G44A) mutation of MED 12 gene exon in leiomyoma tissues than healthy myometrium tissues of same patients. There
was a 3.7-fold decrease in the expression of microRNA-124 in leiomyoma tissues compared to intact eutopic myometrium
tissues, but this difference was not statistically significant.
Conclusions: In recent studies, it has been suggested that MED 12 gene may play an active role in the formation of
fibroids. MED12 and β-catenin / Wnt pathway were emphasized, and alternative genetic pathways are sought in fibroid
formation. Also, tumour suppressor and oncogenesis effects of microRNAs have been demonstrated in many different
studies. Since it is involved in the Wnt pathway, microRNA-124 has been blamed by some previous studies for the formation
of fibroids. This study demonstrates that MED12 exon 2 mutations and probably microRNA-124 gene expressions might
contribute to uterine leiomyoma pathology.

Abstract

Objectives: It is believed that there are still unclear areas in the formation mechanism of leiomyomas. In our study, it
was aimed to investigate the formation mechanisms of leiomyomas due to local MED 12 gene exon 2 mutation and local
microRNA-124 expression in a Turkish population.
Material and methods: Thirty patients who underwent hysterectomy for leiomyoma uteri at Gaziantep University between
January 2013 and January 2016 were included in our study. In the pathology specimens of these patients, the patient’s
myometrium tissue and her own leiomyoma tissue were analysed via quantitative Realtime PCR in association with MED
12 exon 2 mutation and microRNA-124 expression.
Results: The average age of the 30 patients included in our study is 46.67 ± 5.42 and 13 patients had single leiomyoma;
17 patients had more than one leiomyoma. There were significantly higher c.130G> T (p.G44C) mutation and c.131G> A
(p.G44A) mutation of MED 12 gene exon in leiomyoma tissues than healthy myometrium tissues of same patients. There
was a 3.7-fold decrease in the expression of microRNA-124 in leiomyoma tissues compared to intact eutopic myometrium
tissues, but this difference was not statistically significant.
Conclusions: In recent studies, it has been suggested that MED 12 gene may play an active role in the formation of
fibroids. MED12 and β-catenin / Wnt pathway were emphasized, and alternative genetic pathways are sought in fibroid
formation. Also, tumour suppressor and oncogenesis effects of microRNAs have been demonstrated in many different
studies. Since it is involved in the Wnt pathway, microRNA-124 has been blamed by some previous studies for the formation
of fibroids. This study demonstrates that MED12 exon 2 mutations and probably microRNA-124 gene expressions might
contribute to uterine leiomyoma pathology.

Get Citation

Keywords

anormal uterin bleeding; leiomyoma; MED 12; microRNA-124

About this article
Title

Comparison of MED 12 gene mutation and microRNA-124 expression in leiomyoma and myometrium of Turkish patients

Journal

Ginekologia Polska

Issue

Vol 93, No 4 (2022)

Article type

Research paper

Pages

290-295

Published online

2022-03-30

Page views

4916

Article views/downloads

557

DOI

10.5603/GP.a2021.0102

Pubmed

35419790

Bibliographic record

Ginekol Pol 2022;93(4):290-295.

Keywords

anormal uterin bleeding
leiomyoma
MED 12
microRNA-124

Authors

Cagdas Demiroglu
Ozge Komurcu Karuserci
Sadrettin Pence
Halime Hanım Pence
Burcu Çaykara
Seyhun Sucu
Mete Gürol Ugur

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