open access

Vol 92, No 9 (2021)
Research paper
Published online: 2021-04-14
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The importance of NFκB1 rs4648068 and RUNX2 rs7771980 polymorphisms in bone metabolism of postmenopausal Polish women

Anna Bogacz12, Aleksandra Gorska3, Adam Kaminski4, Marlena Wolek3, Hubert Wolski56, Agnieszka Seremak-Mrozikiewicz5, Jaroslaw Goracy7, Boguslaw Czerny89
DOI: 10.5603/GP.a2021.0044
·
Pubmed: 33914303
·
Ginekol Pol 2021;92(9):617-623.
Affiliations
  1. Department of Pharmacology and Phytochemistry, Institute of Natural Fibers and Medicinal Plants, Poznan, Poland
  2. Department of Histocompatibility with Laboratory of Genetic Diagnostics, Regional Blood Center, Poznan, Poland
  3. Department of Stem Cells and Regenerative Medicine, Institute of Natural Fibres and Medicinal Plants, Poznan, Poland
  4. Clinic of Pediatric Orthopedics, Pomeranian Medical University, Szczecin, Poland
  5. Division of Perinatology and Women’s Diseases, Poznan University of Medical Sciences, Poznan, Poland
  6. Division of Gynecology and Obstetrics, Podhale Multidisciplinary Hospital, Nowy Targ, Poland
  7. Independent Laboratory of Invasive Cardiology, Pomeranian Medical University in Szczecin, Poland
  8. Department of Stem Cells and Regenerative Medicine, Institute of Natural Fibres and Medicinal Plants, Poznan, Poland
  9. Department of Pharmacology and Pharmacoeconomics, Pomeranian Medical University in Szczecin, Poland

open access

Vol 92, No 9 (2021)
ORIGINAL PAPERS Gynecology
Published online: 2021-04-14

Abstract

Objectives: Osteoporosis is a multifactorial disease that causes a loss of bone density. However, genetic factors play an increasingly important role in its development. To thoroughly understand the molecular mechanisms, polymorphic variants of genes candidate for osteoporosis are still being sought. The aim of our study was to investigate the influence of NFκB1 gene rs4648068 (A>G) and RUNX2 gene rs7771980 (-1025T>C) polymorphisms on the risk of osteoporosis.
Material and methods: A group of 675 postmenopausal Caucasian women (109 women with osteopenia, 333 with osteoporosis and 233 with normal T-score) were examined. The bone mineral density (BMD) at the lumbar spine (L1-L4) was measured by dual energy x-ray absorptiometry (DXA). The analysis of NFκB1 and RUNX2 polymorphisms was performed using real-time PCR method.
Results: Analysis of NFκB1 gene rs4648068 polymorphism showed that the GG genotype was slightly more frequent in the study groups compared to the control group. In the osteoporosis group, patients with the G allele in the genotype have lower bone mineral density values. For the RUNX2 rs7771980 polymorphism, in women with osteopenia we observed an increased incidence of TC heterozygotes compared to the control group (29.40% vs 24.90%, p > 0.05), and in women with osteoporosis, the TT genotype was more common (78.70% vs 73.80%, p > 0.05). No correlation was observed between the genotypes and the clinical parameters.
Conclusions: The analysis showed no significant relationship between the genotypic distribution and the individual clinical parameters. However, it is suggested an association between the rs4648068 polymorphism of the NFκB1 gene and an increased risk of developing osteoporosis.

Abstract

Objectives: Osteoporosis is a multifactorial disease that causes a loss of bone density. However, genetic factors play an increasingly important role in its development. To thoroughly understand the molecular mechanisms, polymorphic variants of genes candidate for osteoporosis are still being sought. The aim of our study was to investigate the influence of NFκB1 gene rs4648068 (A>G) and RUNX2 gene rs7771980 (-1025T>C) polymorphisms on the risk of osteoporosis.
Material and methods: A group of 675 postmenopausal Caucasian women (109 women with osteopenia, 333 with osteoporosis and 233 with normal T-score) were examined. The bone mineral density (BMD) at the lumbar spine (L1-L4) was measured by dual energy x-ray absorptiometry (DXA). The analysis of NFκB1 and RUNX2 polymorphisms was performed using real-time PCR method.
Results: Analysis of NFκB1 gene rs4648068 polymorphism showed that the GG genotype was slightly more frequent in the study groups compared to the control group. In the osteoporosis group, patients with the G allele in the genotype have lower bone mineral density values. For the RUNX2 rs7771980 polymorphism, in women with osteopenia we observed an increased incidence of TC heterozygotes compared to the control group (29.40% vs 24.90%, p > 0.05), and in women with osteoporosis, the TT genotype was more common (78.70% vs 73.80%, p > 0.05). No correlation was observed between the genotypes and the clinical parameters.
Conclusions: The analysis showed no significant relationship between the genotypic distribution and the individual clinical parameters. However, it is suggested an association between the rs4648068 polymorphism of the NFκB1 gene and an increased risk of developing osteoporosis.

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Keywords

osteoporosis; polymorphism; NFκB1; RUNX2; postmenopausal women

About this article
Title

The importance of NFκB1 rs4648068 and RUNX2 rs7771980 polymorphisms in bone metabolism of postmenopausal Polish women

Journal

Ginekologia Polska

Issue

Vol 92, No 9 (2021)

Article type

Research paper

Pages

617-623

Published online

2021-04-14

DOI

10.5603/GP.a2021.0044

Pubmed

33914303

Bibliographic record

Ginekol Pol 2021;92(9):617-623.

Keywords

osteoporosis
polymorphism
NFκB1
RUNX2
postmenopausal women

Authors

Anna Bogacz
Aleksandra Gorska
Adam Kaminski
Marlena Wolek
Hubert Wolski
Agnieszka Seremak-Mrozikiewicz
Jaroslaw Goracy
Boguslaw Czerny

References (16)
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  16. Vaughan T, Reid DM, Morrison NA, et al. RUNX2 alleles associated with BMD in Scottish women; interaction of RUNX2 alleles with menopausal status and body mass index. Bone. 2004; 34(6): 1029–1036.

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