Increased osteopontin expression in endometrial carcinoma is associated with better survival outcome
, 12, 13
Abstract
Objectives: Osteopontin (OPN) is a key extracellular matrix protein that is involved in cancer progression. The aim of the
current study is to investigate the relation of OPN immunostaining in endometrial carcinoma with clinicopathological
parameters.
Material and methods: Archival 71 endometrial carcinomas and 30 non-neoplastic endometrial tissues were obtained from
the Department of Pathology at King Abdulaziz University Jeddah, Saudi Arabia. Tissue microarrays were constructed. Tissue
sections were stained using anti-human OPN monoclonal antibody. Immunostaining results were recorded and analysed.
Results: In non-neoplastic endometrial tissues, high (increased) OPN immunostaining was observed in 100%. In endometrial
carcinoma, high (increased) OPN immunostaining was seen in 64.8% of cases. High (increased) OPN immunostaining
was more frequent in non-neoplastic tissues than in endometrial carcinoma (p < 0.001). OPN immunostaining showed no
association with histological type, FIGO tumour grade, tumour size, myometrial invasion, lymphovascular invasion, surgical
resection margin or lymph node metastasis. On the other hand, high (increased) OPN immunostaining was associated with
better overall survival [Log Rank (Mantel-Cox) = 4.385, p = 0.003].
Conclusions: In endometrial carcinoma, immunohistochemical staining of OPN could be a helpful tool in the prediction
survival pattern. OPN immunostaining showed no association with most clinicopathological features. Further investigations
both clinical and molecular are needed to explore the downstream of OPN in endometrial carcinoma.
Keywords: endometrial carcinomatissue microarrayimmunohistochemistryosteopontin
References
- Du Xl, Jiang T, Sheng Xg, et al. Inhibition of osteopontin suppresses in vitro and in vivo angiogenesis in endometrial cancer. Gynecol Oncol. 2009; 115(3): 371–376.
- Smyth NA, Murawski CD, Adams SB, et al. International Consensus Group on Cartilage Repair of the Ankle. Osteochondral Allograft: Proceedings of the International Consensus Meeting on Cartilage Repair of the Ankle. Foot Ankle Int. 2018; 39(1_suppl): 35S–40S.
- Brown LF, Berse B, Van de Water L, et al. Expression and distribution of osteopontin in human tissues: widespread association with luminal epithelial surfaces. Mol Biol Cell. 1992; 3(10): 1169–1180.
- Ritter NM, Farach-Carson MC, Butler WT. Evidence for the formation of a complex between osteopontin and osteocalcin. J Bone Miner Res. 1992; 7(8): 877–885.
- Rodrigues LR, Teixeira JA, Schmitt FL, et al. The role of osteopontin in tumor progression and metastasis in breast cancer. Cancer Epidemiol Biomarkers Prev. 2007; 16(6): 1087–1097.
- Philip S, Bulbule A, Kundu GC. Osteopontin stimulates tumor growth and activation of promatrix metalloproteinase-2 through nuclear factor-kappa B-mediated induction of membrane type 1 matrix metalloproteinase in murine melanoma cells. J Biol Chem. 2001; 276(48): 44926–44935.
- Li Y, Xie Y, Cui D, et al. Osteopontin Promotes Invasion, Migration and Epithelial-Mesenchymal Transition of Human Endometrial Carcinoma Cell HEC-1A Through AKT and ERK1/2 Signaling. Cell Physiol Biochem. 2015; 37(4): 1503–1512.
- Hahne JC, Meyer SR, Kranke P, et al. Studies on the role of osteopontin-1 in endometrial cancer cell lines. Strahlenther Onkol. 2013; 189(12): 1040–1048.
- Senger D, Wirth D, Hynes R. Transformed mammalian cells secrete specific proteins and phosphoproteins. Cell. 1979; 16(4): 885–893.
- Assidi M, Gomaa W, Jafri M, et al. Prognostic value of Osteopontin (SPP1) in colorectal carcinoma requires a personalized molecular approach. Tumour Biol. 2019; 41(9): 1010428319863627.
- Furger KA, Menon RK, Tuck AB, et al. The functional and clinical roles of osteopontin in cancer and metastasis. Curr Mol Med. 2001; 1(5): 621–632.
- Ue T, Yokozaki H, Kitadai Y, et al. Co-expression of osteopontin and CD44v9 in gastric cancer. Int J Cancer. 1998; 79(2): 127–132.
- Chambers AF, Wilson SM, Kerkvliet N, et al. Osteopontin expression in lung cancer. Lung Cancer. 1996; 15(3): 311–323.
- Tuck AB, O'Malley FP, Singhal H, et al. Osteopontin expression in a group of lymph node negative breast cancer patients. Int J Cancer. 1998; 79(5): 502–508.
- Rittling SR, Chambers AF. Role of osteopontin in tumour progression. Br J Cancer. 2004; 90(10): 1877–1881.
- Gomaa W, Al-Ahwal M, Hamour O, et al. Osteopontin cytoplasmic immunoexpression is a predictor of poor disease-free survival in thyroid cancer. Journal of Microscopy and Ultrastructure. 2013; 1(1): 8.
- Al-Maghrabi J, Emam E, Gomaa W, et al. c-MET immunostaining in colorectal carcinoma is associated with local disease recurrence. BMC Cancer. 2015; 15: 676.
- Gomaa W, Ke Y, Fujii H, et al. Tissue microarray of head and neck squamous carcinoma: validation of the methodology for the study of cutaneous fatty acid-binding protein, vascular endothelial growth factor, involucrin and Ki-67. Virchows Arch. 2005; 447(4): 701–709.
- Gomaa W, Al-Ahwal M, Hamour O, et al. Osteopontin cytoplasmic immunoexpression is a predictor of poor disease-free survival in thyroid cancer. Journal of Microscopy and Ultrastructure. 2013; 1(1): 8.
- Wei R, Wong JP, Kwok HF. Osteopontin -- a promising biomarker for cancer therapy. J Cancer. 2017; 8(12): 2173–2183.
- Castello LM, Raineri D, Salmi L, et al. Osteopontin at the Crossroads of Inflammation and Tumor Progression. Mediators Inflamm. 2017; 2017: 4049098.
- Briese J, Schulte HM, Bamberger CM, et al. Expression pattern of osteopontin in endometrial carcinoma: correlation with expression of the adhesion molecule CEACAM1. Int J Gynecol Pathol. 2006; 25(2): 161–169.
- von Wolff M, Strowitzki T, Becker V, et al. Endometrial osteopontin, a ligand of beta3-integrin, is maximally expressed around the time of the "implantation window". Fertil Steril. 2001; 76(4): 775–781.
- Casals G, Ordi J, Creus M, et al. Osteopontin and alphavbeta3 integrin as markers of endometrial receptivity: the effect of different hormone therapies. Reprod Biomed Online. 2010; 21(3): 349–359.
- Casals G, Ordi J, Creus M, et al. Osteopontin and alphavbeta3 integrin expression in the endometrium of infertile and fertile women. Reprod Biomed Online. 2008; 16(6): 808–816.
- Cho H, Kang ES, Kim YT, et al. Diagnostic and prognostic impact of osteopontin expression in endometrial cancer. Cancer Invest. 2009; 27(3): 313–323.
- Lax SF. Molecular genetic pathways in various types of endometrial carcinoma: from a phenotypical to a molecular-based classification. Virchows Arch. 2004; 444(3): 213–223.
- Hashiguchi Y, Tsuda H, Bandera CA, et al. Comparison of osteopontin expression in endometrioid endometrial cancer and ovarian endometrioid cancer. Med Oncol. 2006; 23(2): 205–212.
- Ramachandran S, Kwon KY, Shin SJ, et al. Regulatory role of osteopontin in malignant transformation of endometrial cancer. Mol Biol Rep. 2013; 40(5): 3623–3629.
